The identification of wheat genetic resources with high dietary fiber content

The quality properties of different variety mixtures and composite cross populations were studied with the aim of identifying genotypes with high dietary fiber content and to cultivate and examine the effect of these components on the end-use quality. Based on the results of a Europe-wide trial, we could detect two populations and variety mixtures which had significantly higher total (TOTAX) and water extractable arabinoxylan (WEAX) content, than most of the studied genotypes, with positive effect on the human health. These populations/mixtures are promising dietary fiber resources and suitable not only for organic but also for conventional farming, especially in Central Europe. The seeds of the best population (Mv Elit CCP) was multiplied to supply it for interested farmers in Hungary in the frame of the European trial on organic heterogeneous materials

The Chemical Genetic Interactions of Statin Drugs with Their Target Genes in Saccharomyces cerevisiae

Statins, competitive inhibitors of the rate limiting cholesterol/ergosterol enzymes HMG-CoA reductase (HMG1 and HMG2), are the most widely prescribed human therapeutic drugs. They are effective in lowering cholesterol levels in atherosclerosis and related syndromes. However, statins exhibit a range of pleiotropic side effects whose mechanisms are poorly understood. This study investigates statin pleiotropy by analysis of genetic interaction networks in yeast, Saccharomyces cerevisiae, which shows high homology to mammalian pathways affected by statins. Synthetic genetic array (SGA) analysis allows elucidation of functional genetic networks of genes of interest ("query genes") by measurement of genetic epistasis in double mutants of the query gene with the genome - wide deletion mutant array of ~4800 non-essential strains. Chemicalgenetic profiling is similar where a SMP may effectively replace the query gene in genome wide epistatic analysis. The genetic interaction networks resulting from use of HMG1 and HMG2 as query genes for SGA analysis were compared to the chemical-genetic profiles of atorvastatin, cerivastatin and lovastatin. The genes ARV1, BTS1, OPI3 displaying phenotypic enhancements (i.e. their deletion caused major growth inhibition) with statins became essential in the presence of all the statins. Two mitochondrial genes, COX17 and MMM1, showed phenotypic suppressions (i.e. their deletion allowed better growth) in common to all three statin drugs. An attractive hypothesis is that major pleiotropic effects of statins could be due to variation in function or expression of these enhancing or suppressing genes. Other processes compensating statin use were also elucidated. For example, when HMG1 and its epistatically interacting genes are shut down by deletion coupled with inhibition of HMG2 with statin, there is strong evidence that the cell attempts to maintain membrane/lipid homeostasis via anterograde and retrograde transport mechanisms, including the mobilisation of lipid storage droplets. To aid refinement of genetic analysis in this and future studies, a more direct phenotypic assay was developed for quantifying ergosterol. Such an assay may be used as a phenotype to map the effect of up - and downstream - genes, or network genes affecting ergosterol levels. This assay was used to quantify ergosterol in a drug - resistant mutant developed by others aiding confirmation of the drug target

Expanding Access to Consumer Health Information: A Multi-Institutional Collaboration

A partnership between an academic library, a public library system, and an area health education center meet a critical information need in a rural region of southeast Georgia

An ArcGIS Tool for Modeling the Climate Envelope with Feed-Forward ANN

This paper is about the development and the application of an ESRI ArcGIS tool which implements multi-layer, feed-forward artificial neural network (ANN) to study the climate envelope of species. The supervised learning is achieved by backpropagation algorithm. Based on the distribution and the grids of the climate (and edaphic data) of the reference and future periods the tool predicts the future potential distribution of the studied species. The trained network can be saved and loaded. A modeling result based on the distribution of European larch (Larix decidua Mill.) is presented as a case study

Sustainable Selection, Breeding and Hatchery Operation for Darag Native Chicken Production in Western Visayas, Philippines

Farmers in Panay Island raise Philippine native chickens known as Darag. However, raisers encounter problems on low fertility and hatchability, including high chick mortality when reared in high population densities. To address these concerns, this study aimed to establish a free range breeder flock, collect data on breeding and reproductive performance of Darag breeders, and develop protocols on culling and selection of breeders, hatchery, and range management. A 100-hen Darag nucleus farm and three 200-hen commercial Darag breeder flocks were established. Heritability estimates, reproductive, feed supplementation, and weights were computed. Results showed the following: heritability estimates for Darag plumage (h2=0.835), brown egg color (h2=0.534), yellow skin color (h2=0.452), gray shank color (h2=0.493), and pale-red earlobes (h2=0.524). Other results are as follows: Age at point of lay, 19–24 weeks; egg produced per hen per year, 96–110; age when productive, 24–36 months; mean daily feed consumption, 50–75 g per bird; mean weight of eggs, 43.04 g; egg breakage, 0.5%–3.5%; viable eggs, 90%–99%; and egg fertility rate, 80.0%–96%. The mortality rates of the chickens at different stages: brooding stage, 4.17%; hardening stage, 5.68%; and growing stage, 7.5%. The cost to production is as follows: egg, PhP 4.17–6.25; day-old chicks, PhP 39.25–47.01; and ready-to-lay pullet/ready-to-breed cockerel, PhP 137.24–143.65. Our calculations reveal a production cost of PhP 796,530.00 for a 100-hen nucleus farm in 42 months and total sales of PhP 998,840.00, with a profit of PhP 202,310.00 and return on investment of 25.40%. A ranging area of 10 m2 per breeder and 1:5 male-female ratio family units are stocking recommendation. Protocols on culling and selection of breeders, hatchery, and range management were developed.

Social integration and financial inclusion of forcibly displaced persons in Sub-Saharan African countries

Most government and international financial institutions worldwide have adopted financial inclusion as a veritable platform for achieving the Social Development Goals of hunger and poverty eradication, inequality reduction, and employment creation. Their efforts will not yield much dividend if a sizeable part of the populace are constrained from social and formal financial inclusion due to social disorder. This study examined the relationship between social seclusion of forcibly displaced persons from formal financial inclusion in twenty-seven Sub-Saharan African countries. Granger Error Correction Method (ECM) with Generalized Methods of Moments (GMM) was used to analyze the short panel data obtained from the World Bank database. The study found a negative long-run relationship between social seclusion and financial inclusion. That is, an increase in social menace overtime will result in more people being financially excluded from formal financial transactions. It, therefore, recommends, amongst others, that government should encourage forcibly displaced persons to become gainfully employed and productive. Specifically, persons in refugee and internally displaced persons camps should be trained to acquire skills that will enable them to become self-employed, create wealth for themselves, and contribute actively to the sustainable economic growth of their host country rather than just provide food and other welfare packages as a temporal palliative for surviva

Author Correction: A ferroptosis–based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis

Correction to: Scientific Reports https://doi.org/10.1038/s41598-019-39739-5, published online 27 February 201

Stability analysis of wheat lines with increased level of arabinoxylan

Plant breeders have long sought to develop lines that combine outstanding performance with high and stable quality in different environments. The high-arabinoxylan (AX) Chinese variety Yumai-34 was crossed with three Central European wheat varieties (Lupus, Mv-Mambo, Ukrainka) and 31 selected high-AX lines were compared for physical (hectolitre weight, thousand grain weight, flour yield), compositional (protein content, gluten content, pentosan) and processing quality traits (gluten index, Zeleny sedimentation, Farinograph parameters) in a three-year experiment (2013–2015) in the F7-F9 generations. The stability and heritability of different traits, including the relative effects of the genotype (G) and environment (E), were determined focusing on grain composition. The contents of total and water-soluble pentosans were significantly affected by G, E and G×E interactions, but the heritability of total (TOT)pentosan was significantly lower (0.341) than that of water-extractable (WE)-pentosan (0.825). The main component of the pentosans, the amount and composition (arabinose: xylose ratio) of the arabinoxylan (AX), was primarily determined by the environment and, accordingly, the broader heritability of these parameters were 0.516 and 0.772. However, genotype significantly affected the amount of water-soluble arabinoxylan and its composition and thus the heritability of these traits was also significant (0.840 and 0.721). The genotypes exhibiting higher stability of content of TOT-pentosan also showed more stable contents of WE-pentosan. There was a positive correlation between the stability of contents of WE-pentosan and WE-AX, while the stability of the WE-AX content and AX composition were also strongly correlated. Water absorption was strongly genetically determined with a heritability of 0.829 with the genotype determining 38.67% of the total variance. Many lines were grouped in the GGE biplot, indicating that they did not significantly differ stability