Distribution of some elements in Veronica scutellata L. from Bolu,Turkey: soil-plant interactions

Veronica scutellata L. occurs in moist and wet habitats, such as ponds, marshes and other wetlands. This study was conducted on this species to examine its mineral element uptake status in terms of interactions between soil and plant. Experimental materials were taken from the Southern coast of Black Sea at coordinates 40º36’N and 31º16’E at an altitude of 1400 m above sea level from Bolu – Turkey; using standard methods and plant (root, stem and leaf parts) and soil mineral element measurements (Al, B, Ca, Cu, Fe, K, Mg, Mn, Na, Ni and Zn) were done. During the study, ICP-OES was employed for the measurement of mineral elements. It was observed that considerable amounts of B, Ca, K, Mg, Mn, Na and Zn are accumulated by the plant

Callus induction and plant regeneration from mature embryos of sunflower

Callus development and efficient shoot and root organogenesis were obtained from five different sunflower (Helianthus annuus L.) genotypes: Trakya 80, Trakya 129, Trakya 259, Trakya 2098, and Viniimk 893 1, which are commercially important for Turkey. Plant tissue culture systems were established on Murashige and Skoog (MS) media supplemented with various plant growth regulators using mature embryos of sunflower. For callus induction MS + 1 mg/l 2,4-D, for shoot regeneration MS + 1 mg/l benzyladenine and 0.5 mg/l alpha-naphthaleneacetic acid were used. Callus induction ratios were around 80-92% in all tested genotypes. The Trakya 259 genotype gave the best shoot regeneration response (44%). All regenerated shoots were rooted on MS medium supplemented with 1 mgA indolyl-3-butyric acid and on MS medium without any hormones. Mature embryos could be an alternative source for indirect plant regeneration and gene transfer systems for different sunflower genotypes

Comparative analysis of mature and preproprotein form of thionins in some plant species; bioinformatics approaches

Thionins are one of the most important antimicrobial peptides in broad-range plant defense. A number of studies are present regarding the structural and biological role of mature thionins but preproprotein forms of these molecules have not been extensively studied. Thus, this study aimed to comparatively analyze a total of 56 thionin preproprotein sequences from 14 different plant species. Analyses of primary, secondary and tertiary structures of these forms revealed that preproproteins with "gamma-thionin domain" were relatively shorter and more basic than proteins with "thionin domain" structure. In addition, members of "thionin domain" were more similar to each other than that of "gamma-thionin domain" forms. Sub-cellular localizations of these forms were predicted as extracellular. Structural superposition of precursor and mature thionins showed that a large portion of precursor sequences are cleaved to form a functional protein. Although precursor forms demonstrated the significant structural divergence in modelled species, functional mature forms showed a structural pattern in ?-helices; two ?-helix proteins included the "thionin domain" family while one a-helix proteins contained the "gamma-thionin domain" family. Results of this study will become valuable theoretical knowledge and provide insight in terms of further understanding the formation of mature functional thionins thereby their biological roles. © by PSP2-s2.0-8509387393