Phytochemical and Antimicrobial Activities of the Leaf Oil Extract of Mentha Spicata and its Efficacy in Repelling Mosquito

Synthetic drugs and repellents have been discovered to have adverse toxicity effects apart from the fact that they are no longer efficient due to adaptation of microbes and mosquitoes to them. This study is based on using the extract from local leaves (Mentha spicata plant) as treatment for microbial diseases as well as mosquito repellent. Extract of leaf were studied and screened for the presence of phytochemicals (secondary metabolites) and antimicrobial properties against some fungi and bacteria viz., Pseudomonas aeruginosa, Bacillus Subtilis, Staphylococcus aureus, Aspergillus niger, Escherichia coli and Saccharomyces cerevisiae. The result of the phytochemical screening revealed that the leave extract contained tannin, steroids, flavonoids, terpenoids, phenols and cardiac glycosides while anthraquinones and saponins were not determined. From the microbial analysis the zone of inhibitions indicated that the extract of Mentha spicata plant had strong activity against bacteria and fungi used in this analysis. Mentha spicata oil extract with the highest concentration when introduced into the produced cream had the highest repellency time lasting up to four [4] hours. The chemical constituents of the leaf oil extract were analyzed using Gas chromatography- mass spectroscopy (GC-MS) and the major chemical constituent identified was carvon

Phytochemical and Antimicrobial Activities of the Leaf Oil Extract of Mentha Spicata and its Efficacy in Repelling Mosquito

Synthetic drugs and repellents have been discovered to have adverse toxicity effects apart from the fact that they are no longer efficient due to adaptation of microbes and mosquitoes to them. This study is based on using the extract from local leaves (Mentha spicata plant) as treatment for microbial diseases as well as mosquito repellent. Extract of leaf were studied and screened for the presence of phytochemicals (secondary metabolites) and antimicrobial properties against some fungi and bacteria viz., Pseudomonas aeruginosa, Bacillus Subtilis, Staphylococcus aureus, Aspergillus niger, Escherichia coli and Saccharomyces cerevisiae. The result of the phytochemical screening revealed that the leave extract contained tannin, steroids, flavonoids, terpenoids, phenols and cardiac glycosides while anthraquinones and saponins were not determined. From the microbial analysis the zone of inhibitions indicated that the extract of Mentha spicata plant had strong activity against bacteria and fungi used in this analysis. Mentha spicata oil extract with the highest concentration when introduced into the produced cream had the highest repellency time lasting up to four [4] hours. The chemical constituents of the leaf oil extract were analyzed using Gas chromatography- mass spectroscopy (GC-MS) and the major chemical constituent identified was carvon

Effect of Crystallisation Time on the Synthesis of Zeolite Y from Elefun Kaolinite Clay

In this study, the effect of crystallization time on the synthesis of zeolite Y from Elefun kaolinite clay was investigated. During the synthesis of zeolite Y, the raw Elefun kaolinite clay was beneficiated thoroughly with water for 7 days before it was calcined at a 850oC for 6 hours to thermally convert it to metakaolin. Dealumination of the metakaolin was then carried out using the novel method to achieve a target SiO2/Al2O3 ratio of 6.5. A hydrogel solution was prepared by mixing the dealuminated metakaolin with sodium hydroxide and water in calculated proportions. The mixture was heated to a temperature of 95oc between a time range of 12 to 60 hours. The crystallized product was then washed thoroughly with deionized water and then dried. Analysis was carried out on the dried product sample using two different characterization techniques; X-ray diffraction (XRD) and X-ray fluoresense (XRF). The results from the analysis indicated the formation of zeolite Y from Elefun Kaolinite clay and the maximum crystallization time obtained for the synthesis of zeolite Y from Elefun Kaolinite clay at 95oC was 48 hour

Effect of Crystallisation Time on the Synthesis of Zeolite Y from Elefun Kaolinite Clay

In this study, the effect of crystallization time on the synthesis of zeolite Y from Elefun kaolinite clay was investigated. During the synthesis of zeolite Y, the raw Elefun kaolinite clay was beneficiated thoroughly with water for 7 days before it was calcined at a 850oC for 6 hours to thermally convert it to metakaolin. Dealumination of the metakaolin was then carried out using the novel method to achieve a target SiO2/Al2O3 ratio of 6.5. A hydrogel solution was prepared by mixing the dealuminated metakaolin with sodium hydroxide and water in calculated proportions. The mixture was heated to a temperature of 95oc between a time range of 12 to 60 hours. The crystallized product was then washed thoroughly with deionized water and then dried. Analysis was carried out on the dried product sample using two different characterization techniques; X-ray diffraction (XRD) and X-ray fluoresense (XRF). The results from the analysis indicated the formation of zeolite Y from Elefun Kaolinite clay and the maximum crystallization time obtained for the synthesis of zeolite Y from Elefun Kaolinite clay at 95oC was 48 hours

ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF NEEM LEAVES AND LEMON GRASS ESSENTIAL OIL EXTRACTS

In this study, the antimicrobial activity and phytochemical constituents of neem leaves and lemon grass oil extracts were evaluated. Oil extracts of neem leaves and lemon grass were obtained by solvent extraction method using hexane and ethanol. Antimicrobial activity screening of plants’ oil extracts were conducted using agar well diffusion method and the oil extracts were tested against three gram negative bacteria (Pseudomonas aeruginosa, Klebsiella specie, Escherichia coli), one gram positive bacteria (Staphylococcus aureus) and two fungi (Candida albicans, Rhizopus specie). Phytochemical components of the ethanolic oil extracts were anthocyanin and betacyanin; quinones; terpenoids and acid for lemon grass. In addition to other phytochemicals present in lemon grass ethanolic oil extract, neem ethanolic oil extracts tested positive to flavonoids. Lemon grass oil extract shows high activity against Escherichia coli, Staphylococcus aureus and Candida albicans which are representative of the three categories of microorganisms considered. Neem leaves oil extracts have relatively low activity against most of the selected microorganisms

Combating A36 mild steel corrosion in 1 M H2SO4 medium using watermelon seed oil inhibitor

Corrosion inhibitive performance of the extracted watermelon seed oil on A36 mild steel in 1 M H2SO4 medium, at 305 and 319 K corrosion reaction temperatures, was investigated. Weight loss and inhibition efficiency were determined using gravimetric method while corrosion rate and inhibitoion efficiency were evaluated using potentiodynamic tests. Gravimetric tests showed that watermelon seed oil inhibitor attained a better corrosion inhibition efficiency of 50% at the operating temperature of 305 K compared to an efficiency of 48% obtained at the operating temperature of 319 K. Electrochemical potentiodynamic polarization tests showed that 3 vol/vol% inhibitor concentration gave the most promising corrosion inhibiting results at the operating temperature of 305 K, while 4 vol/vol% inhibitor concentration gave the most reliable corrosion resisting one at the operating temperature of 319 K. Langmuir adsorption isotherm correctly predicted the adsorption behaviour of the watermelon seed oil on A36 mild steel surface in 1 M H2SO4 medium. the negative values of ΔGads revealed the spontaneous adsorption nature of the inhibitor on the mild steel surface and the electrochemical potentiodynamic polarization results showed that the watermelon seed oil acted as a mixed-type corrosion inhibitor. The optical image analysis revealed both the potency level of watermelon seed oil as an inhibitor as well as the optimum inhibitor concentrations of 3 vol/vol% (at 305 K) and 4 vol/vol% (at 319 K)

Bio-Conversion of Sweet Potato Peel Waste to BioEthanol Using Saccharomyces Cerevisiae

The bio-production of ethanol from sweet potato peel using microorganism was examined. Proximate analysis was carried out on the peel and the results from the analysis demonstrated that sweet potato peel contained sufficient amounts of starch and total carbohydrates to guarantee use for bio-ethanol production. To obtain maximum conversion of starch into fermentable sugars, sweet potato peel was hydrolysed by hydrochloric acid at different temperature and concentration. The effect of inoculum size, pH, temperature and time to obtain maximum ethanol from sweet potato peel waste were studied in batch fermentation. The maximum ethanol yield obtained was 6.39g/L at pH 5.0, temperature of 32.5⁰C and inoculums size of 6% (v/v) after 48 hours of fermentation. The results obtained showed that sweet potato peel which is a waste, can be a feedstock for bioethanol production

Dataset on the effect of different pretreatment on the proximate analysis, microbial and sensory evaluation of dried banana during its storage

Pre-treatment on banana slices is usually carried out to stop discolouration of the fruit by oxidative reactions, in raw pre- sentation of the fruit especially in fruit salads. Drying is an old long preservation method for fruits and vegetables. In drying banana fruit, discolorations do occur and an attempt to stop the discoloration while maintaining quality (shelf life) is studied in this work. Dataset presented here, is on the effect of different pretreatment on the proximate analysis, microbial and sensory evaluation of dried banana during its storage. The pre-treatment methods considered in this work, were lemon juice and carbonated lemon drink while the con- trol had no pre-treatment on the slices before drying. Prox- imate analysis and water activity of raw and dried samples were carried out while the microbial and sensory evalua- tion changes were observed in the samples over one month period

Prospective observational cohort study on grading the severity of postoperative complications in global surgery research

Background The Clavien–Dindo classification is perhaps the most widely used approach for reporting postoperative complications in clinical trials. This system classifies complication severity by the treatment provided. However, it is unclear whether the Clavien–Dindo system can be used internationally in studies across differing healthcare systems in high- (HICs) and low- and middle-income countries (LMICs). Methods This was a secondary analysis of the International Surgical Outcomes Study (ISOS), a prospective observational cohort study of elective surgery in adults. Data collection occurred over a 7-day period. Severity of complications was graded using Clavien–Dindo and the simpler ISOS grading (mild, moderate or severe, based on guided investigator judgement). Severity grading was compared using the intraclass correlation coefficient (ICC). Data are presented as frequencies and ICC values (with 95 per cent c.i.). The analysis was stratified by income status of the country, comparing HICs with LMICs. Results A total of 44 814 patients were recruited from 474 hospitals in 27 countries (19 HICs and 8 LMICs). Some 7508 patients (16·8 per cent) experienced at least one postoperative complication, equivalent to 11 664 complications in total. Using the ISOS classification, 5504 of 11 664 complications (47·2 per cent) were graded as mild, 4244 (36·4 per cent) as moderate and 1916 (16·4 per cent) as severe. Using Clavien–Dindo, 6781 of 11 664 complications (58·1 per cent) were graded as I or II, 1740 (14·9 per cent) as III, 2408 (20·6 per cent) as IV and 735 (6·3 per cent) as V. Agreement between classification systems was poor overall (ICC 0·41, 95 per cent c.i. 0·20 to 0·55), and in LMICs (ICC 0·23, 0·05 to 0·38) and HICs (ICC 0·46, 0·25 to 0·59). Conclusion Caution is recommended when using a treatment approach to grade complications in global surgery studies, as this may introduce bias unintentionally

The surgical safety checklist and patient outcomes after surgery: a prospective observational cohort study, systematic review and meta-analysis

© 2017 British Journal of Anaesthesia Background: The surgical safety checklist is widely used to improve the quality of perioperative care. However, clinicians continue to debate the clinical effectiveness of this tool. Methods: Prospective analysis of data from the International Surgical Outcomes Study (ISOS), an international observational study of elective in-patient surgery, accompanied by a systematic review and meta-analysis of published literature. The exposure was surgical safety checklist use. The primary outcome was in-hospital mortality and the secondary outcome was postoperative complications. In the ISOS cohort, a multivariable multi-level generalized linear model was used to test associations. To further contextualise these findings, we included the results from the ISOS cohort in a meta-analysis. Results are reported as odds ratios (OR) with 95% confidence intervals. Results: We included 44 814 patients from 497 hospitals in 27 countries in the ISOS analysis. There were 40 245 (89.8%) patients exposed to the checklist, whilst 7508 (16.8%) sustained ≥1 postoperative complications and 207 (0.5%) died before hospital discharge. Checklist exposure was associated with reduced mortality [odds ratio (OR) 0.49 (0.32–0.77); P\u3c0.01], but no difference in complication rates [OR 1.02 (0.88–1.19); P=0.75]. In a systematic review, we screened 3732 records and identified 11 eligible studies of 453 292 patients including the ISOS cohort. Checklist exposure was associated with both reduced postoperative mortality [OR 0.75 (0.62–0.92); P\u3c0.01; I2=87%] and reduced complication rates [OR 0.73 (0.61–0.88); P\u3c0.01; I2=89%). Conclusions: Patients exposed to a surgical safety checklist experience better postoperative outcomes, but this could simply reflect wider quality of care in hospitals where checklist use is routine