A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors

A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations

Massive Star Formation in the Tarantula Nebula

In this work, we present 299 candidate young stellar objects (YSOs) in 30 Doradus discovered using Spitzer and Herschel point-source catalogs, 276 of which are new. We study the parental giant molecular clouds in which these YSO candidates form using recently published Atacama Large Millimeter/submillimeter Array (ALMA) Cycle 7 observations of ^12 CO and ^13 CO. The threshold for star formation in 30 Doradus inferred by the LTE-based mass surface density is 178 M _⊙ pc ^−2 , 40% higher than the threshold for star formation in the Milky Way. This increase in star formation threshold in comparison to the Milky Way and increase in line width seen in clumps 11 pc away in comparison to clumps 45 pc away from the R136 super star cluster could be due to injected turbulent energy, increase in interstellar medium pressure, and/or local magnetic field strength. Of the 299 YSO candidates in this work, 62% are not associated with ^12 CO molecular gas. This large fraction can be explained by the fact that 75%–97% of the H _2 gas is not traced by CO. We fit a Kroupa initial mass function to the YSO candidates and find that the total integrated stellar mass is 18,000 M _⊙ and that the region has a star formation rate (SFR) of 0.18 M _⊙ yr ^−1 . The initial mass function determined here applies to the four 150″ × 150″ (37.5 pc × 37.5 pc) subfields and one 150″ × 75″ (37.5 pc × 18.8 pc) subfield observed with ALMA. The SFR in 30 Doradus has increased in the past few million years

The structure and dynamics of the 30 Doradus molecular cloud as revealed by ALMA

International audienceWe present results of a wide-field (approximately 60 × 90 pc) ALMA mosaic of CO(2-1) and 13CO(2-1) emission from the molecular cloud associated with the 30 Doradus star-forming region. Three main emission complexes, including two forming a bowtie-shaped structure extending northeast and southwest from the central R136 cluster, are resolved into complex filamentary networks. Consistent with previous studies, we find that the central region of the cloud has higher line widths at fixed size relative to the rest of the molecular cloud and to other LMC clouds, indicating an enhanced level of turbulent motions. However, there is no clear trend in gravitational boundedness (as measured by the virial parameter) with distance from R136. Structures observed in 13CO are spatially coincident with filaments and are close to a state of virial equilibrium. In contrast, 12CO structures vary greatly in virialization, with low CO surface brightness structures outside of the main filamentary network being predominantly unbound. The low surface brightness structures constitute ~10% of the measured CO luminosity; they may be shredded remnants of previously star-forming gas clumps, or alternatively the CO-emitting parts of more massive, CO-dark structures

The structure and dynamics of the 30 Doradus molecular cloud as revealed by ALMA

International audienceWe present results of a wide-field (approximately 60 × 90 pc) ALMA mosaic of CO(2-1) and 13CO(2-1) emission from the molecular cloud associated with the 30 Doradus star-forming region. Three main emission complexes, including two forming a bowtie-shaped structure extending northeast and southwest from the central R136 cluster, are resolved into complex filamentary networks. Consistent with previous studies, we find that the central region of the cloud has higher line widths at fixed size relative to the rest of the molecular cloud and to other LMC clouds, indicating an enhanced level of turbulent motions. However, there is no clear trend in gravitational boundedness (as measured by the virial parameter) with distance from R136. Structures observed in 13CO are spatially coincident with filaments and are close to a state of virial equilibrium. In contrast, 12CO structures vary greatly in virialization, with low CO surface brightness structures outside of the main filamentary network being predominantly unbound. The low surface brightness structures constitute ~10% of the measured CO luminosity; they may be shredded remnants of previously star-forming gas clumps, or alternatively the CO-emitting parts of more massive, CO-dark structures

The 30 Doradus Molecular Cloud at 0.4 pc Resolution with the Atacama Large Millimeter/submillimeter Array: Physical Properties and the Boundedness of CO-emitting Structures

International audienceWe present results of a wide-field (approximately 60 × 90 pc) Atacama Large Millimeter/submillimeter Array mosaic of CO(2-1) and 13CO(2-1) emission from the molecular cloud associated with the 30 Doradus star-forming region in the Large Magellanic Cloud (LMC). Three main emission complexes, including two forming a bow-tie-shaped structure extending northeast and southwest from the central R136 cluster, are resolved into complex filamentary networks. Consistent with previous studies, we find that the central region of the cloud has higher line widths at a fixed size relative to the rest of the molecular cloud and to other LMC clouds, indicating an enhanced level of turbulent motions. However, there is no clear trend in gravitational boundedness (as measured by the virial parameter) with distance from R136. Structures observed in 13CO are spatially coincident with filaments and are close to a state of virial equilibrium. In contrast, 12CO structures vary greatly in virialization, with low CO surface brightness structures outside of the main filamentary network being predominantly unbound. The low surface brightness structures constitute ~10% of the measured CO luminosity; they may be shredded remnants of previously star-forming gas clumps, or alternatively the CO-emitting parts of more massive, CO-dark structures