Variabilite De La Coloration Du Tegument Des Graines De Voandzou Cultivees Au Burkina Faso Et Les Noms Locaux Associes

Vigna subterranea (L.) Verdcourt specie is a traditional African legume, grown in all parts of Burkina Faso. It plays a huge role in the resilience of poor people to food and nutritional insecurity. In order to contribute to the safeguarding of the genetic resources of this specie and to better valorize it in the selection and varietal improvement programs, a study on the phenotypic variability based on the coloration of the seed coat and the nomenclature of the local varieties within certain ethnic groups has been realized. Prospecting and collection of bambara groundnut accessions has made in several localities across the three agro-climatic zones of Burkina Faso. Accessions collected were identified on the basis of seed coat colouring and local names within some ethnic groups. Frequency calculations of the accessions collected according to the staining of tegument were performed. From a total of 138 seed samples collected 322 accessions were obtained with a breakdown into 22 groups of accessions according to the coloration of their tegument. This important phenotypic variability can be exploited in the breeding and varietal breeding programs of bambara groundnut. Knowing the local names of the accessions can also be useful in the nomenclature of the selected varieties and can help in facilitating their adoption by the producers

Molecular characterization of the main fungi associated to Bambara groundnut foliar diseases in Burkina Faso

Objective: This study aims to update the database of fungi associated to Bambara groundnut foliar diseases in Burkina Faso using both molecular and morphological identification approaches.Methodology and Results: In this study, molecular approach based on the sequencing of ITS (Internal Transcripted Spacer) region of fungi and  morphological approach were used to identify the main fungi associated to Bambara groundnut foliar diseases. The study was performed with universal polymerase chain reaction (PCR) primer ITS1/ITS4. BlastN comparisons between 19 fungal isolates contigs of the 16 major fungi were produced by their DNA sequences assembly and GenBank sequences yielded identity scores of 99 to 100 % with all of them. The degrees of similarity between these contigs and the loci sequences of classified fungi in GenBank indicate that our fungal isolates are the same species with those in Genbank, particularly the first of the list show after the blastN. It is the first report of molecular characterization of the main fungi infecting Bambara groundnut in Burkina Faso.Conclusion and Application of results: Nineteen fungi associated to  Bambara groundnut foliar diseases were identified and can be taken as targets in varietal improvement of Bambara groundnut for resistance to fungal diseases in Burkina Faso.Key words: Bambara groundnut, fungi, molecular characterization, PCR primer ITS1/ITS4, Burkina Faso