154 research outputs found
Implicit Regularization and Renormalization of QCD
We apply the Implicit Regularization Technique (IR) in a non-abelian gauge
theory. We show that IR preserves gauge symmetry as encoded in relations
between the renormalizations constants required by the Slavnov-Taylor
identities at the one loop level of QCD. Moreover, we show that the technique
handles divergencies in massive and massless QFT on equal footing.Comment: (11 pages, 2 figures
Prospecção de novos biossurfactantes a partir da biblioteca metagenômica de manguezal utilizando High Throughput Screening (HTS).
Partitioning and purification of polygalacturonases produced by Aspergillus niger URM 5162 using PEG-phosphate in an aqueous two-phase system
Pectinases, or pectinolytic enzymes, are naturally produced by plants, filamentous fungi, bacteria and yeasts. The pectinases are
of great importance to clarify and reduce viscosity in fruit juices, improving and increasing tbe filtration efficiency. When used
in the crushing of grapes or wine must improve juice extraction, reduce the time to clarify and enhance tbe content ofterpenes in
wine. The filamentous fungi most frequently used fur industrial purposes because as much as 90% ofthe enzyme can be excreted
into the culture medium. The partitioning and purification of polygalacturonases (PG) produced by Aspergillus niger URM
5162 were investigated in aqueous two-phase systems (ATPS), furmed by polyetbylene glycol and phosphate salts
(PE(ijlhosphate). To evaluate the effect oftbe 4 independent variables- molar mass ofpolyetbylene glycol (PEG) (400-8000
g1nol MPEG), PEG concentration (12.5-17.5%, w/w- CPEG), phosphate concentration (15-25%, ...W, CPHOS) and pH (6.0, 8.0)
- on the 3 response variables: partition coefficient (K), activity yield (Y) and purification fàctor (PF), a fuctorial design (24) was
used. The endo-polygalacturonases (endo-PG) were prefurentially partitioned in tbe top phase. For endo-PG, the highest values
for the response variables K, Y and PF of 1.23, 74.04% and 8.18, respectively, were obtained for a CPEG of 12.5% (...W), MPEG
of8000 g1nol, and CPHOS of25% (w/w) at pH 6.0. Also, exo-polygalacturonases (exo-PG) were preferentially partitioned in the
top phase. ln tbis case, the highest values ofK (2.40), Y (33.33%), and PF (1.98) were obtained with a MPEG of 8000 g1nol,
CPEG of 12.5% (...W), and CPHOS of25% (...W) at pH 6.0. ln both cases, MPEG had a positive influence on K, Y and PF. The
conditions ofMPEG 8000 g1nol, CPEG of 12.5% (...W), and CPHOS of25% (...W) at pH 6.0 were considered the most suitable
for tbe purification of PG produced by A. niger URM 5162. Furtbermore, MPEG and CPHOS were the most important
independent variables. The PEG/phosphate system is a useful cost-effective altemative for PG purification
Screening of bacterial isolates from Brazilian oil-impacted mangrove for lipase and esterase activities.
Mitochondrial Haplogroup H1 in North Africa: An Early Holocene Arrival from Iberia
The Tuareg of the Fezzan region (Libya) are characterized by an extremely high frequency (61%) of haplogroup H1, a mitochondrial DNA (mtDNA) haplogroup that is common in all Western European populations. To define how and when H1 spread from Europe to North Africa up to the Central Sahara, in Fezzan, we investigated the complete mitochondrial genomes of eleven Libyan Tuareg belonging to H1. Coalescence time estimates suggest an arrival of the European H1 mtDNAs at about 8,000–9,000 years ago, while phylogenetic analyses reveal three novel H1 branches, termed H1v, H1w and H1x, which appear to be specific for North African populations, but whose frequencies can be extremely different even in relatively close Tuareg villages. Overall, these findings support the scenario of an arrival of haplogroup H1 in North Africa from Iberia at the beginning of the Holocene, as a consequence of the improvement in climate conditions after the Younger Dryas cold snap, followed by in situ formation of local H1 sub-haplogroups. This process of autochthonous differentiation continues in the Libyan Tuareg who, probably due to isolation and recent founder events, are characterized by village-specific maternal mtDNA lineages
A checklist of native freshwater fish from Brantas River, East Java, Indonesia
Hasan V, Mamat NB, South J, Ottoni FP, Widodo MS, Arisandi P, Isroni W, Jerikho R, Samitra D, Faqih AR, Simanjuntak CPH, Mukti AT. 2022. A checklist of native freshwater fish from Brantas River, East Java, Indonesia. Biodiversitas 23: 6031-6039. This study aims to provide information on the freshwater fish species composition of the Brantas River, the second-largest river in Java, Indonesia. All samples used in this study were the caught activities during Fish Resource Survey (form May 5, 2021 to August 20, 2022). Sampling was carried out in four stations of the Brantas River, representing upstream, the Karangkates Reservoir, midstream, and downstream. This work recorded 42 fish species divided into 35 genera and 21 families. Cyprinidae was the dominant family, comprising 12 genera and 15 species. According to the IUCN Red List, most fish species inhabiting the Brantas River have a conservation status of Least Concern (LC) or Data Deficient (DD), except for Rasbora lateristriata which is categorized as Vulnerable (VU). Genera Tor and Neolissochilus are rarely found in Javanese waters. We suggest that the conservation status of these species should be reviewed. Information on habitat use and ecological interactions of fish occurring in the Brantas River are needed urgently to make appropriate conservation decisions and plans. Therefore, we suggest that more collections need to be carried out in the studied river, as well as studies based on eDNA analysis and research incorporating both morphological and molecular data
Uniparental markers of contemporary Italian population reveals details on its pre-Roman heritage.
BACKGROUND: According to archaeological records and historical documentation, Italy has been a melting point for populations of different geographical and ethnic matrices. Although Italy has been a favorite subject for numerous population genetic studies, genetic patterns have never been analyzed comprehensively, including uniparental and autosomal markers throughout the country.
METHODS/PRINCIPAL FINDINGS: A total of 583 individuals were sampled from across the Italian Peninsula, from ten distant (if homogeneous by language) ethnic communities--and from two linguistic isolates (Ladins, Grecani Salentini). All samples were first typed for the mitochondrial DNA (mtDNA) control region and selected coding region SNPs (mtSNPs). This data was pooled for analysis with 3,778 mtDNA control-region profiles collected from the literature. Secondly, a set of Y-chromosome SNPs and STRs were also analyzed in 479 individuals together with a panel of autosomal ancestry informative markers (AIMs) from 441 samples. The resulting genetic record reveals clines of genetic frequencies laid according to the latitude slant along continental Italy--probably generated by demographical events dating back to the Neolithic. The Ladins showed distinctive, if more recent structure. The Neolithic contribution was estimated for the Y-chromosome as 14.5% and for mtDNA as 10.5%. Y-chromosome data showed larger differentiation between North, Center and South than mtDNA. AIMs detected a minor sub-Saharan component; this is however higher than for other European non-Mediterranean populations. The same signal of sub-Saharan heritage was also evident in uniparental markers.
CONCLUSIONS/SIGNIFICANCE: Italy shows patterns of molecular variation mirroring other European countries, although some heterogeneity exists based on different analysis and molecular markers. From North to South, Italy shows clinal patterns that were most likely modulated during Neolithic times
Randomized study of adjuvant chemotherapy for completely resected stage I, II, or IIIA non-small-cell lung cancer
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