Building on an oasis in Garamantian times: geoarchaeological investigation on mud architectural elements from the excavation of Fewet (Central Sahara, SW Libya)

The paper describes the micromorphological and mineralogical properties of earthen architectural elements from the excavation of the Garamantian compound of Fewet (Central Sahara, SW Libya), settled between the 2nd century BC and the 1st century AD, and compares this evidence with a set of samples from historical to modern context of Saharan and Sub-Saharan Africa. At Fewet, the production of mud bricks, plasters, and mortars employed in the building of the compound required raw materials available near the settlement. The earthen elements lack almost completely clay and organic temper, and their main components are quartz grains (sandy to silty) and a calcareous and slightly organic mud, available beside former springs. Only plaster and mortars show the addition (in limited quantity) of finely subdivided vegetal remains to the mixture. The technology for earthen elements used in Garamantian times resembles those today applied at many localities in Sub-Saharan Africa. Finally, our analyses showed that in the last millennia archaeological sediments underwent limited postdepositional weathering, mostly related to solute redistribution after occasional rainfalls. Today, the same process affects traditional mud brick buildings

Monocyte Distribution Width (MDW) as novel inflammatory marker with prognostic significance in COVID-19 patients

Monocyte Distribution Width (MDW), a new cytometric parameter correlating with cytomorphologic changes occurring upon massive monocyte activation, has recently emerged as promising early biomarker of sepsis. Similar to sepsis, monocyte/macrophage subsets are considered key mediators of the life-threatening hyper-inflammatory disorder characterizing severe COVID-19. In this study, we longitudinally analyzed MDW values in a cohort of 87 COVID-19 patients consecutively admitted to our hospital, showing significant correlations between MDW and common inflammatory markers, namely CRP (p < 0.001), fibrinogen (p < 0.001) and ferritin (p < 0.01). Moreover, high MDW values resulted to be prognostically associated with fatal outcome in COVID-19 patients (AUC = 0.76, 95% CI: 0.66\u20130.87, sensitivity 0.75, specificity 0.70, MDW threshold 26.4; RR = 4.91, 95% CI: 1.73\u201313.96; OR = 7.14, 95% CI: 2.06\u201324.71). This pilot study shows that MDW can be useful in the monitoring of COVID-19 patients, as this innovative hematologic biomarker is: (1) easy to obtain, (2) directly related to the activation state of a fundamental inflammatory cell subset (i.e. monocytes, pivotal in both cytokine storm and sepsis immunopathogenesis), (3) well correlated with clinical severity of COVID-19-associated inflammatory disorder, and, in turn, (4) endowed with relevant prognostic significance. Additional studies are needed to define further the clinical impact of MDW testing in the management of COVID-19 patients

Multiparametric flow cytometry for MRD monitoring in hematologic malignancies: Clinical applications and new challenges

In hematologic cancers, Minimal Residual Disease (MRD) monitoring, using either molecular (PCR) or immunophenotypic (MFC) diagnostics, allows the identification of rare cancer cells, readily detectable either in the bone marrow or in the peripheral blood at very low levels, far below the limit of classic microscopy. In this paper, we outlined the state-of-the-art of MFC-based MRD detection in different hematologic settings, highlighting main recommendations and new challenges for using such a method in patients with acute leukemias or chronic hematologic neoplasms. The combination of new molecular technologies with advanced flow cytometry is progressively allowing clinicians to design a personalized therapeutic path, proportionate to the biological aggressiveness of the disease, in particular by using novel immunotherapies, in view of a modern decision-making process, based on precision medicine. Along with the evolution of immunophenotypic and molecular diagnostics, the assessment of Minimal Residual Disease (MRD) has progressively become a keystone in the clinical management of hematologic malignancies, enabling valuable post-therapy risk stratifications and guiding risk-adapted therapeutic approaches. However, specific prognostic values of MRD in different hematological settings, as well as its appropriate clinical uses (basically, when to measure it and how to deal with different MRD levels), still need further investigations, aiming to improve standardization and harmonization of MRD monitoring protocols and MRD-driven therapeutic strategies. Currently, MRD measurement in hematological neoplasms with bone marrow involvement is based on advanced highly sensitive methods, able to detect either specific genetic abnormalities (by PCRbased techniques and next-generation sequencing) or tumor-associated immunophenotypic profiles (by multiparametric flow cytometry, MFC). In this review, we focus on the growing clinical role for MFC-MRD diagnostics in hematological malignancies-from acute myeloid and lymphoblastic leukemias (AML, B-ALL and T-ALL), to chronic lymphocytic leukemia (CLL) and multiple myeloma (MM)-providing a comparative overview on technical aspects, clinical implications, advantages and pitfalls of MFC-MRD monitoring in different clinical settings

Challenges for a Sustainable Food Supply Chain: A Review on Food Losses and Waste

To address global food security, new strategies are required in view of the challenges represented by Climate Change, depletion of natural resources and the need to not further compromise the ecosystems’ quality and biodiversity. Food losses and waste (FLW) affect food security and nutrition, as well as the sustainability of food systems. Quantification of the adverse effects of FLW is a complex and multidimensional challenge requiring a wide-ranging approach, regarding the quantification of FLW as well as the related economic, environmental and social aspects. The evaluation of suitable corrective actions for managing FLW along the food supply chain requires a system of sound and shared benchmarks that seem still undefined. This review aims to provide an overview of the environmental, economic and social issues of FLW, which may support policy measures for prevention, reduction and valorization of food wastes within the food supply chain. In fact, detection of the hotspots and critical points allows to develop tailored policy measures that may improve the efficiency of the food supply chain and its sustainability, with an integrated approach involving all the main actors and considering the several production contexts

L'area sepolcrale della terramara S.Rosa di Poviglio (RE. Contesto, materiali,riti

During the 2000 field season, several fragments of calcined human bone were recovered in the excavation of a modern ditch around 300 m to the east of the edge of the terramara of Santa Rosa. These brought to light the location of a funerary area related to the site. During the following years (2001 and 2002) a 7000 sq m area was investigated in detail through trenches and excavation pits. The archaeological structures recovered were related to a soil that was also in use during the Iron Age and Roman period and was later buried below Medieval alluvial deposits. The archaeological evidence consists of four cinerary urns, two secondary deposits of parts of a single skull included in two different pits, and several holes of different shapes, many of which containing organic material. Furthermore, the buried soil is cut by irrigation ditches and includes, dispersed in it, several calcined human bones fragments, very few animal bones and some pottery sherds. The cinerary urns - two of which are related to an anomalous burial ritual - contain the remains of two adults and two children. Three phases are distinguishable in the use of the area, ranging from the Middle to the Recent Bronze Age: during the earliest phase, the ditches were excavated to irrigate the area, which was devoted to an agrarian use; after that, the four cinerary urns were deposited; later, probably after the dispersal of pyre remains on the surface of the soil, the holes were opened in part to host wooden posts, and two of them included skull fragments, deposited as votive offer

In-vitro activity of cefpodoxime proxetil (RU 51807): a comparative disk diffusion study on isolates from geriatric patients

997 strains isolated from clinical specimens arrived at the "Pio Albergo Trivulzio" microbiology laboratory were tested using disks of cefpodoxime, amoxicillin + clavulanic acid, cefaclor, cefuroxime, ofloxacin, cotrimoxazole, ceftriaxone and cefalexin. Gram-positive strains were tested also with erythromycin, while gram-negative bacteria were tested against aztreonam. Cefpodoxime overall activity was well above the effectiveness of the other oral cephalosporins and on the same order as ceftriaxone and ofloxacin. Cefpodoxime proved to be also more active than the combination amoxicillin-clavulanic acid

Abnormal scattergrams and cell population data generated by fully automated hematological analyzers: New tools for screening malaria infection?

INTRODUCTION: Malaria is a life-threatening infectious disease, which has been for long confined to specific endemic areas. Nevertheless, the recent increase in immigration flows from endemic regions and imported cases has reemphasized many diagnostic challenges in Western countries, thus paving the way to introduce rapid and accurate strategies for screening subjects with suspected Malaria infection. Therefore, the aim of this article was to describe our recent experience with Sysmex XN-module for rapid screening of subjects with suspected Malaria. METHODS: Fourteen patients admitted to the Emergency Department (Papa Giovanni XXIII Hospital Bergamo, Italy) with a clinical suspicion of Malaria infection were evaluated, along with 1047 control samples. The analysis of peripheral blood was performed with XN-module, and results were then compared to optical microscopy. RESULTS: Nine patients were positive to Plasmodim falciparum, 3 to Plasmodim vivax, one to Plasmodim ovale, and one to Plasmodim malarie. Characteristic abnormalities could be observed in both white blood cell differential (WDF) and white cell nucleated (WNR) scattergrams (sensitivity 0.64 and specificity 1.0) in 9 samples with parasites at gametocyte or schizos stage irrespective of Plasmodium species and parasitic index, while characteristic scattergram abnormalities could not be seen in the 5 samples containing only parasites at the trophozoites stage. In these cases, specific variations of some cell population data (CPD) could be recorded (sensitivity 1.00 and specificity 0.91). CONCLUSION: The peculiar abnormalities observed in CPDs, WDF, and WNR-scattergrams may raise a definite suspicion of Malaria infection. Further studies should then be planned for validating these preliminary findings and assessing whether these specific abnormalities may be incorporated in rapid and inexpensive Malaria diagnostic algorithms

Cultural ecosystem services: A review of methods and tools for economic evaluation

Cultural ecosystem services (CES) are non-material intangible benefits that humans derive from ecosystems, which are indispensable for the well-being of communities and directly influence the quality of life. CES are deeply interconnected to each other and to providing and regulating services, thus influencing everyday life. CES are among the most important values that people associate with nature, but understanding them may be challenging. The definition of CES is both self-evident and elusive, specifically because they consist of the interaction between two dynamic systems: human societies and natural ecosystems. This paper updates the state of the art about CES evaluation methods, underlining the gap between their economic values and their incorporation into planning and decision-making on different scales and in different sectors, and emphasizes their importance in conservation policies and sustainable development programs. This study reviewed 68 articles published between August 2019 and May 2023 from the SCOPUS database, and classified CES assessment into 15 evaluation methods. This review reveals that the choice of CES assessment methodologies has often depended on evaluation purposes. In addition, specific CES classifications are required, since different definitions and unstandardized economic concepts for assigning market values to the CES can lead to conflicting results. The combination of different methods, monetary and non-monetary, can aid better evaluation of CES by focusing on the interaction between different components, and can facilitate the mapping and quantification of social values of ecosystem services. This can help decision-makers to develop sustainable territorial planning and policies