Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats

Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. OBJECTIVES: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. MATERIAL AND METHODS: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. RESULTS: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). CONCLUSIONS: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics

Characterization and in vitro cytocompatibility of an acid-etched titanium surface

The aims of this study were to characterize the microstructure of a commercially pure titanium (cpTi) surface etched with HCl/H2SO4 (AE-cpTi) and to investigate its in vitro cytocompatibility compared to turned cpTi (T-cpTi). T-cpTi showed a grooved surface and AE-cpTi revealed a surface characterized by the presence of micropits. Surface parameters indicated that the AE-cpTi surface is more isotropic and present a greater area compared to T-cpTi. The oxide film thickness was similar between both surfaces; however, AE-cpTi presented more Ti and O and less C. Osteoblastic cell proliferation, alkaline phosphatase activity, and bone-like nodule formation were greater on T-cpTi than on AE-cpTi. These results show that acid etching treatment produced a surface with different topographical and chemical features compared to the turned one, and such surface modification affected negatively the in vitro cytocompatibility of cpTi as demonstrated by decreasing culture growth and expression of osteoblastic phenotype.O objetivo deste estudo foi caracterizar a microestrutura de uma superfície de titânio comercialmente puro (cpTi) condicionada com HCl/H2SO4 (acid etched) (AE-cpTi) e investigar sua citocompatibilidade in vitro, comparada à do cpTi usinado (turned) (T-cpTi). O T-cpTi apresentou uma superfície com sulcos e o AE-cpTi exibiu uma superfície caracterizada pela presença de micro-vales. Os parâmetros de superfície indicaram que a superfície AE-cpTi é mais isotrópica e apresenta uma área maior quando comparada à superfície T-cpTi. A espessura da camada de óxido foi similar para as duas superfícies; no entanto, a AE-cpTi apresentou maiores quantidades de Ti e O e menor, de C. A proliferação de células osteoblásticas, a atividade de fosfatase alcalina e a formação de matriz mineralizada foram maiores na superfície T-cpTi que na AE-cpTi. Esses resultados mostram que o condicionamento ácido produziu uma superfície com características topográficas e químicas diferentes quando comparadas às da superfície usinada. Além disso, observou-se que essas modificações de superfície afetaram de forma negativa a citocompatibilidade in vitro do cpTi como demonstrado pela inibição da proliferação celular e da expressão do fenótipo osteoblástico

RANCANG BANGUN ANEMOMETER BERBASIS AKUISISI DATA

Data acquisition system is aimed to display value and graphic in real-time. It is designed on Anemometer to present value or graphic of wind speed on Personal Computer (PC) that furthermore, data is analyzed and stored automatically in Microsoft Excel. Equipment used is Wind Speed Air Velocity Anemometer in the form of fan to measure wind speed. It is designed by using Optocoupler as transducer connected to Arduino Uno ATmega328. This Optocoupler has integrated phototransistor and LED (Light Emitting Diode) components. Both components functioned as receiver and data transfer of wind speed from fan by 0 and 1 logic value. Obtained data by PC will be shown on table and graphic including complete time. The result of test shows accuracy specification of Anemometer in 96,149 % and 1,619 % precision with average deviation standard of 0,0475

RECONSTRUÇÃO CIRÚRGICA COM ENXERTO DE CRISTA ILÍACA DE UM AMELOBLASTOMA MULTICÍSTICO - RELATO DE CASO

O Ameloblastoma multicístico é um tumor Odontogênico benigno que acomete os maxilares apresentando-se de forma agressiva, necessitando de tratamento imediato que na grande maioria das vezes deixa sequelas que impossibilitam a reabilitação protética do paciente

FECHAMENTO DE COMUNICAÇÃO BUCOSSINUSAL INFECTADA: RELATO DE CASO

A comunicação bucossinusal após exodontia é um tipo de complicação que deve ser revertida o mais prontamente possível, a fim de minimizar os riscos de infecções do seio maxilar

Histologic study of use of microfibrillar collagen hemostat in rat dental sockets

The aim of this paper was to evaluate if the placement of microfibrillar collagen hemostat (MCH) into a dental socket interfered with healing. General anesthesia was administered to 30 adult male Albinus Wistar rats and the maxillary right central incisor was extracted. In the control group after each tooth was extracted, the socket was sutured. In the MCH group after each tooth was extracted, MCH was placed into the socket before suturing. Postoperatively, 5 animals were sacrificed from each group at 7, 21 and 28 days. The right maxilla was removed from each animal and histologic slides were stained with Masson's trichromic and hematoxylin and eosin. Quantitative and qualitative analyses were done. The percentage of bone area in the dental socket was quantified using the Image Lab 98 image analysis system. The bone area formation for the control and MCH groups was: 8.1% and 3.3% at 7 days, 34.4% and 33% at 21 days and 41% and 41.3% at 28 days, respectively. We concluded that MCH interferes with the beginning of dental socket healing but does not interfere with the final healing of the dental socket

Histologic study of use of microfibrillar collagen hemostat in rat dental sockets

O objetivo deste trabalho é avaliar se a colocação do MCH no interior do alvéolo dental interfere ou não no reparo alveolar. Foi feita anestesia geral nos trinta ratos machos adultos Albinus Wistar e o incisivo central superior direito foi extraído. No grupo controle, após a exodontia o alvéolo foi suturado. No grupo MCH, após a exodontia introduziu-se MCH dentro do alvéolo que foi suturado. No pós-operatório, cinco animais de cada grupo foram sacrificados aos 7, 21 e 28 dias. Retirou-se a maxila direita de cada animal e cortes histológicos foram confeccionados. Foi feita análise qualitativa e quantitativa. A porcentagem de área óssea no interior dos alvéolos dentais foi quantificada usando o sistema de análise de imagens ImageLab 98. A área óssea neoformada no grupo controle e no grupo MCH foi: 8.1% e 3.3% aos 7 dias, 34.4% e 33% aos 21 dias e 41% e 41.3% aos 28 dias, respectivamente. Dentro das condições experimentais deste trabalho foi possível concluir que o MCH interfere durante o início do reparo alveolar e não interfere no reparo final do alvéolo dental.The aim of this paper was to evaluate if the placement of microfibrillar collagen hemostat (MCH) into a dental socket interfered with healing. General anesthesia was administered to 30 adult male Albinus Wistar rats and the maxillary right central incisor was extracted. In the control group after each tooth was extracted, the socket was sutured. In the MCH group after each tooth was extracted, MCH was placed into the socket before suturing. Postoperatively, 5 animals were sacrificed from each group at 7, 21 and 28 days. The right maxilla was removed from each animal and histologic slides were stained with Masson's trichromic and hematoxylin and eosin. Quantitative and qualitative analyses were done. The percentage of bone area in the dental socket was quantified using the Image Lab 98 image analysis system. The bone area formation for the control and MCH groups was: 8.1% and 3.3% at 7 days, 34.4% and 33% at 21 days and 41% and 41.3% at 28 days, respectively. We concluded that MCH interferes with the beginning of dental socket healing but does not interfere with the final healing of the dental socket.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq