325 MHz VLA Observations of Ultracool Dwarfs TVLM 513-46546 and 2MASS J0036+1821104

We present 325 MHz (90 cm wavelength) radio observations of ultracool dwarfs TVLM 513-46546 and 2MASS J0036+1821104 using the Very Large Array (VLA) in June 2007. Ultracool dwarfs are expected to be undetectable at radio frequencies, yet observations at 8.5 GHz (3.5 cm) and 4.9 GHz (6 cm) of have revealed sources with > 100 {\mu}Jy quiescent radio flux and > 1 mJy pulses coincident with stellar rotation. The anomalous emission is likely a combination of gyrosynchrotron and cyclotron maser processes in a long-duration, large-scale magnetic field. Since the characteristic frequency for each process scales directly with the magnetic field magnitude, emission at lower frequencies may be detectable from regions with weaker field strength. We detect no significant radio emission at 325 MHz from TVLM 513-46546 or 2MASS J0036+1821104 over multiple stellar rotations, establishing 2.5{\sigma} total flux limits of 795 {\mu}Jy and 942 {\mu}Jy respectively. Analysis of an archival VLA 1.4 GHz observation of 2MASS J0036+1821104 from January 2005 also yields a non-detection at the level of < 130 {\mu}Jy . The combined radio observation history (0.3 GHz to 8.5 GHz) for these sources suggests a continuum emission spectrum for ultracool dwarfs which is either flat or inverted below 2-3 GHz. Further, if the cyclotron maser instability is responsible for the pulsed radio emission observed on some ultracool dwarfs, our low-frequency non-detections suggest that the active region responsible for the high-frequency bursts is confined within 2 stellar radii and driven by electron beams with energies less than 5 keV.Comment: 11 pages, 5 figures, submitted to A

Discovery of Radio Emission from the Tight M8 Binary: LP 349-25

We present radio observations of 8 ultracool dwarfs with a narrow spectral type range (M8-M9.5) using the Very Large Array at 8.5 GHz. Only the tight M8 binary LP 349-25 was detected. LP 349-25 is the tenth ultracool dwarf system detected in radio and its trigonometric parallax pi = 67.6 mas, recently measured by Gatewood et al., makes it the furthest ultracool system detected by the Very Large Array to date, and the most radio-luminous outside of obvious flaring activity or variability. With a separation of only 1.8 AU, masses of the components of LP 349-25 can be measured precisely without any theoretical assumptions (Forveille et al.), allowing us to clarify their fully-convective status and hence the kind of magnetic dynamo in these components which may play an important role to explain our detection of radio emission from these objects. This also makes LP 349-25 an excellent target for further studies with better constraints on the correlations between X-ray, radio emission and stellar parameters such as mass, age, temperature, and luminosity in ultracool dwarfs.Comment: accepted by ApJ, referee's comments included, typo in equation 1 correcte

Protein synthesis-dependent formation of protein kinase Mζ in long-term potentiation

The maintenance of long-term potentiation (LTP) in the CA1 region of the hippocampus has been reported to require both a persistent increase in phosphorylation and the synthesis of new proteins. The increased activity of protein kinase C (PKC) during the maintenance phase of LTP may result from the formation of PKMζ, the constitutively active fragment of a specific PKC isozyme. To define the relationship among PKMζ, longterm EPSP responses, and the requirement for new protein synthesis, we examined the regulation of PKMζ after subthreshold stimulation that produced short-term potentiation (STP) and after suprathreshold stimulation by single and multiple tetanic trains that produced LTP. We found that, although no persistent increase in PKMζ followed STP, the degree of long-term EPSP potentiation was linearly correlated with the increase of PKMζ. The increase was first observed 10 min after a tetanus that induced LTP and lasted for at least 2 hr, in parallel with the persistence of EPSP enhancement. Both the maintenance of LTP and the long-term increase in PKMζ were blocked by the protein synthesis inhibitors anisomycin and cycloheximide. These results suggest that PKMζ is a component of a protein synthesis-dependent mechanism for persistent phosphorylation in LTP

Thermodynamics of C incorporation on Si(100) from ab initio calculations

We study the thermodynamics of C incorporation on Si(100), a system where strain and chemical effects are both important. Our analysis is based on first-principles atomistic calculations to obtain the important lowest energy structures, and a classical effective Hamiltonian which is employed to represent the long-range strain effects and incorporate the thermodynamic aspects. We determine the equilibrium phase diagram in temperature and C chemical potential, which allows us to predict the mesoscopic structure of the system that should be observed under experimentally relevant conditions.Comment: 5 pages, 3 figure

Mapping brain circuitry with a light microscope

The beginning of the 21st century has seen a renaissance in light microscopy and anatomical tract tracing that together are rapidly advancing our understanding of the form and function of neuronal circuits. The introduction of instruments for automated imaging of whole mouse brains, new cell type–specific and trans-synaptic tracers, and computational methods for handling the whole-brain data sets has opened the door to neuroanatomical studies at an unprecedented scale. We present an overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function

Radio Astronomy in LSST Era

A community meeting on the topic of "Radio Astronomy in the LSST Era" was hosted by the National Radio Astronomy Observatory in Charlottesville, VA (2013 May 6--8). The focus of the workshop was on time domain radio astronomy and sky surveys. For the time domain, the extent to which radio and visible wavelength observations are required to understand several classes of transients was stressed, but there are also classes of radio transients for which no visible wavelength counterpart is yet known, providing an opportunity for discovery. From the LSST perspective, the LSST is expected to generate as many as 1 million alerts nightly, which will require even more selective specification and identification of the classes and characteristics of transients that can warrant follow up, at radio or any wavelength. The LSST will also conduct a deep survey of the sky, producing a catalog expected to contain over 38 billion objects in it. Deep radio wavelength sky surveys will also be conducted on a comparable time scale, and radio and visible wavelength observations are part of the multi-wavelength approach needed to classify and understand these objects. Radio wavelengths are valuable because they are unaffected by dust obscuration and, for galaxies, contain contributions both from star formation and from active galactic nuclei. The workshop touched on several other topics, on which there was consensus including the placement of other LSST "Deep Drilling Fields," inter-operability of software tools, and the challenge of filtering and exploiting the LSST data stream. There were also topics for which there was insufficient time for full discussion or for which no consensus was reached, which included the procedures for following up on LSST observations and the nature for future support of researchers desiring to use LSST data products.Comment: Conference summary, 29 pages, 1 figure; to be published in the Publ. Astron. Soc. Pacific; full science program and presentations available at http://science.nrao.edu/science/event/RALSST201

Actin/alpha-actinin-dependent transport of AMPA receptors in dendritic spines: role of the PDZ-LIM protein RIL

The efficacy of excitatory transmission in the brain depends to a large extent on synaptic AMPA receptors, hence the importance of understanding the delivery and recycling of the receptors at the synaptic sites. Here we report a novel regulation of the AMPA receptor transport by a PDZ (postsynaptic density-95/Drosophila disc large tumor suppressor zona occludens 1) and LIM (Lin11/rat Isl-1/Mec3) domain-containing protein, RIL (reversion-induced LIM protein). We show that RIL binds to the AMPA glutamate receptor subunit GluR-A C-terminal peptide via its LIM domain and to alpha-actinin via its PDZ domain. RIL is enriched in the postsynaptic density fraction isolated from rat forebrain, strongly localizes to dendritic spines in cultured neurons, and coprecipitates, together with alpha-actinin, in a protein complex isolated by immunoprecipitation of AMPA receptors from forebrain synaptosomes. Functionally, in heterologous cells, RIL links AMPA receptors to the alpha-actinin/actin cytoskeleton, an effect that appears to apply selectively to the endosomal surface-internalized population of the receptors. In cultured neurons, an overexpression of recombinant RIL increases the accumulation of AMPA receptors in dendritic spines, both at the total level, as assessed by immunodetection of endogenous GluR-A-containing receptors, and at the synaptic surface, as assessed by recording of miniature EPSCs. Our results thus indicate that RIL directs the transport of GluR-A-containing AMPA receptors to and/or within dendritic spines, in an alpha-actinin/actin-dependent manner, and that such trafficking function promotes the synaptic accumulation of the receptors