Repertoire and clonality of t-cell receptor beta variable genes expressed in endometrium and blood t cells of patients with recurrent spontaneous abortion

Problem: Recurrent spontaneous abortion (RSA) is a relatively common disorder, the underlying causes of which are thought to be immunological in most cases. Method of Study: Expression profile and clonality pattern of T-cell receptor beta variable (TCRBV) genes in endometrium and blood of patients with RSA were investigated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) using BV gene-specific primers. Relative expression of each BV family was determined and clonal expansion of the over-expressed genes was assessed by analysis of CDR3 length polymorphism. Results: Compared to blood, relative expression of four TCRBV genes was significantly higher in the endometrium of RSA group. Over-expressed genes, except for TCRBV3, all had restricted and oligoclonal patterns of expression in the endometrium. Conclusion: Endometrial T cells have a skewed TCRBV repertoire with restricted transcript heterogeneity, which is shared by both groups and minor variations observed in this pattern in RSA patients may reflect more recent and/or repeated exposure to nominal antigens or superantigens. © 2008 The Authors Journal compilation © 2008 Blackwell Munksgaard

Immunogenetics of Disease-Causing Inflammation in Sarcoidosis

none4sinoneGrunewald, Johan; Spagnolo, Paolo; Wahlström, Jan; Eklund, AndersGrunewald, Johan; Spagnolo, Paolo; Wahlström, Jan; Eklund, Ander