Rapid MPN-Qpcr Screening for Pathogens in Air, Soil, Water, and Agricultural Produce

A sensitive, high-throughput, and cost-effective method for screening bacterial pathogens in the environment was developed. A variety of environmental samples, including aerosols, soil of various types (sand, sand/clay mix, and clay), wastewater, and vegetable surface (modeled by tomato), were concomitantly spiked with Salmonella enterica and/or Pseudomonas aeruginosa to determine recovery rates and limits of detection. The various matrices were first enriched with a general pre-enrichment broth in a dilution series and then enumerated by most probable number (MPN) estimation using quantitative PCR for rapid screening of amplicon presence. Soil and aerosols were then tested in non-spiked environmental samples, as these matrices are prone to large experimental variation. Limit of detection in the various soil types was 1–3 colony-forming units (CFU) g[superscript −1]; on vegetable surface, 5 CFU per tomato; in treated wastewater, 5 CFU L[superscript −1]; and in aerosols, >300 CFU mL[superscript −1]. Our method accurately identified S. enterica in non-spiked environmental soil samples within a day, while traditional methods took 4 to 5 days and required sorting through biochemically and morphologically similar species. Likewise, our method successfully identified P. aeruginosa in non-spiked aerosols generated by a domestic wastewater treatment system. The obtained results suggest that the developed method presents a broad approach for the rapid, efficient, and reliable detection of relatively low densities of pathogenic organisms in challenging environmental samples.United States-Israel Binational Agricultural Research and Development Fund (Grant No. CP-9033-09)MIT International Science and Technology InitiativesKraft Foods Compan

A cross-country psychiatric screening of ICD-11 disorders specifically associated with stress in Kenya, Nigeria and Ghana

Background The Global Forum for Health Research, with the support of the World Health Organization, highlighted the need to prioritize mental health research in Africa. The introduction of revised descriptions of Posttraumatic Stress Disorder (PTSD) and Adjustment Disorder, along with new diagnoses of Complex PTSD and Prolonged Grief Disorder, in the ICD-11 creates a need for additional national level epidemiological studies on the prevalence of stress-related disorders.Methods The prevalence rates of these four ICD-11 stress disorders were assessed in three African countries including Nigeria (N = 1006), Kenya (N = 1018), and Ghana (N = 500). Participants completed disorder-specific measures for each disorder.Findings Across the entire sample, the current prevalence rate of probable Adjustment Disorder was 8.4% (95% C.I. = 7.4%, 9.6%), probable PTSD was 18.6% (95% C.I. = 17.2, 20.2%), probable Complex PTSD was 15.9% (95% C.I. = 14.5%, 17.4%) and probable Prolonged Grief Disorder was 3.7% (95% C.I. = 3.1%, 4.5%).Interpretation The results are applicable primarily to well-educated urban and suburban adults in these African countries. Results indicated that Adjustment Disorder, PTSD, and CPTSD are highly prevalent in these three African countries. There is now a pressing need to develop culturally sensitive interventions to enable recovery from these conditions

Modulated expression of WFDC1 during carcinogenesis and cellular senescence

Fibroblasts located adjacent to the tumor [cancer-associated fibroblasts (CAFs)] that constitute a large proportion of the cancer-associated stroma facilitate the transformation process. In this study, we compared the biological behavior of CAFs that were isolated from a prostate tumor to their normal-associated fibroblast (NAF) counterparts. CAFs formed more colonies when seeded at low cell density, exhibited a higher proliferation rate and were less prone to contact inhibition. In contrast to the general notion that high levels of α-smooth muscle actin serve as a marker for CAFs, we found that prostate CAFs express it at a lower level compared with prostate NAFs. Microarray analysis revealed a set of 161 genes that were altered in CAFs compared with NAFs. We focused on whey acidic protein four-disulfide core domain 1 (WFDC1), a known secreted protease inhibitor, and found it to be downregulated in the CAFs. WFDC1 expression was also dramatically downregulated in highly prolific mesenchymal cells and in various cancers including fibrosarcomas and in tumors of the lung, bladder and brain. Overexpression of WFDC1 inhibited the growth rate of the fibrosarcoma HT1080 cell line. Furthermore, WFDC1 level was upregulated in senescent fibroblasts. Taken together, our data suggest an important role for WFDC1 in inhibiting proliferation of both tumors and senescent cells. Finally, we suggest that the downregulation of WFDC1 might serve as a biomarker for cellular transformation

Mutant p53 facilitates somatic cell reprogramming and augments the malignant potential of reprogrammed cells

p53 deficiency enhances the efficiency of somatic cell reprogramming to a pluripotent state. As p53 is usually mutated in human tumors and many mutated forms of p53 gain novel activities, we studied the influence of mutant p53 (mut-p53) on somatic cell reprogramming. Our data indicate a novel gain of function (GOF) property for mut-p53, which markedly enhanced the efficiency of the reprogramming process compared with p53 deficiency. Importantly, this novel activity of mut-p53 induced alterations in the characteristics of the reprogrammed cells. Although p53 knockout (KO) cells reprogrammed with only Oct4 and Sox2 maintained their pluripotent capacity in vivo, reprogrammed cells expressing mutant p53 lost this capability and gave rise to malignant tumors. This novel GOF of mut-p53 is not attributed to its effect on proliferation, as both p53 KO and mut-p53 cells displayed similar proliferation rates. In addition, we demonstrate an oncogenic activity of Klf4, as its overexpression in either p53 KO or mut-p53 cells induced aggressive tumors. Overall, our data show that reprogrammed cells with the capacity to differentiate into the three germ layers in vitro can form malignant tumors, suggesting that in genetically unstable cells, such as those in which p53 is mutated, reprogramming may result in the generation of cells with malignant tumor-forming potential

Screening of adjustment disorder: Scale based on the ICD-11 and the Adjustment Disorder New Module

In line with ICD-11 new conceptualization of Adjustment disorder (AjD), a self-report Adjustment Disorder-New Module (ADNM) was developed and validated. Nevertheless, the ADNM-20 is a long research tool and potentially problematic in the use in epidemiological and clinical studies. The present study introduces the brief ADNM-8 and the ultra-brief ADNM-4, examines their validity and establishes cut-off scores for their clinical use. The study used a representative national sample of 1003 Israelis who reported on the ICD-11 stress spectrum ranging from AjD, PTSD, complex PTSD and complicated grief. Construct validity was assessed via confirmatory factor analysis and cut-off scores were established through ROC analysis. The original and brief instruments were highly correlated (r > 0.918 or better). Cronbach's Alpha for the Brief ADNM-8 and the Ultra-Brief ADNM-4 were above 0.800. Correlations with stress related conditions indicated a good convergent and construct validity for both instruments as well. The ultra-brief ADNM-4 was found to have a very good fit with the data. These findings indicate that the brief ADNM-8 and the ultra-brief ADNM-4 can serve as a brief screening tools for assessing AjD symptoms according to the ICD-11 definition

Super Typhoon

Data set after the Super Typhoon. Can be used to verify our results. Any request to use the data for publication should be addressed to Prof. Ben-Ezra as this data is being used for publication. <br><br>Prof. Menachem Ben-Ezra<br>[email protected]<br

From Hiroshima to Fukushima : PTSD symptoms and radiation stigma across regions in Japan

Stigma is known to be associated with poorer mental health (Raguram et al., 1996). Perceived radiation stigma is the belief that people who were exposed to radiation are contaminated and are discriminated against (Tone and Stone, 2014). Japan is the only country that experienced two major nuclear disasters: the first the A-bomb attacks on Hiroshima and Nagasaki during WWII, the second the Fukushima Daiichi nuclear disaster in 2011 (Ben-Ezra et al., 2012). In Japanese radiation exposure is “Hibaku”; this term, along with “Hibakusha” (an exposed individual), has been used to stigmatize A-bomb survivors and now it is used with respect to the Fukushima Daiichi nuclear disaster in 2011

Typhoon survivors' subjective wellbeing—A different view of responses to natural disaster

<div><p>Objective</p><p>Subjective well-being was evaluated three weeks after Super Typhoon Haiyan struck the Philippines. Based on the Conservation of Resources theory, which focuses on the role of resources in understanding adjustment following trauma, data was collected on lost resources. In line with the Conservation of Resources theory, four categories of resources were defined: objects—residential property; condition—gender health state and witness to injury; personal—coping strategies; energy–relationships.</p><p>Design and settings</p><p>Eight hundred thirty-four people from the Philippines filled out self-report measures using an online interview system regarding: socio demographics data, subjective well-being, using the Delighted Terrible Faces Scale (DTS), disaster related experiences, coping strategies, personal relationships, obtained through support sources (close family, relatives and friends, community) and assessing problems with those relationships after Haiyan.</p><p>Results</p><p>Subjective well-being was predicted by the following classes of resources: objects (home damage) condition (self-rated health and witness to injury), personal (positive reframing and self-blame coping strategies) and energy resources (relations and problems in relations).</p><p>Conclusions</p><p>The results imply the important role individual’s resources (i.e. objects, personal characteristics, conditions, and energies) might play in promoting subjective well-being, following natural disaster.</p></div