Особенности течения и эффективности превентивной терапии гестоза при многократных родах

Objective: to study the course of gestosis in multiple labors and the impact of preventive therapy (a prospective study).Subjects. A hundred and fifty-eight females in the third trimester, including 108 multiparas with gestosis were examined (a study group). The study group was also divided in 2 subgroups: 1) 58 multipara pregnant females having a complete prenatal preparation in accordance with the current reasons for the treatment of gestosis and for the prevention of its complications; 2) 50 multipara pregnant females having a partial antenatal preparation due to late admission or to the onset of labor activity, which required accelerated delivery. A control group comprised 50 multiparas without gestosis.Methods. Clinical, statistical, and calorimetric studies using sets of commercial reagents (Boenringer Mannheim (Germany)); plasma hematocrit (determination of OTsK) and calculation (determination of OPTs and OTsE) methods.Results. Complex preventive therapy used in multiparas with gestosis promoted the occurrence of clinical remission in 82.7% of subgroup 1 pregnant females. After therapy, the critical forms of gestosis, diagnosed in 16% of subgroup 2 pregnant females, were observed in only 5.2% of subgroup 1 females. However, in patients with baseline severe nephropathy, the effect was temporary, which testified to a severe impairment of protective and adaptive mechanisms and to significant catabolism in multiparas with gestosis. The values of OTsK, OPTs, and OTsE in multiparas tended to decrease particularly in subgroup 2 of the study group. In multipara, progressive gestosis was accompanied by a pronounced decrease in the blood levels of proteins with a relatively compensatory rise in their concentration and, to a lesser extent, in that of globulins, suggesting the tension of metabolic processes in the body. Gestosis in multipara was attended by impairments in all hemostatic links, as compared to the control group. There was a significant decrease in the venous blood count of platelets, an increase in the level of hematocrit, and a reduction in the times of clotting and recalcification; there were also rises in the prothrombin index and fibrinogen levels.Conclusion. The findings lead to the conclusion that hemodynamic and hemostatic disorders are reversible when the treatment for mild and moderate gestosis is correct and is initiated in due time. At the same time, no benefit from the performed therapy testifies to the stability and refractoriness of metabolic shifts in severe gestosis during multiple labors. Цель исследования — изучить течение гестоза при многократных родах и влияние предлагаемой превентивной терапии (проспективное исследование).Материалы исследования. Обследовано 158 женщин в III триместре беременности, из них 108 многорожавших женщин с гестозом — основная группа. Основная группа также была разделена на 2 подгруппы: 1-я подгруппа — 58 многорожавших беременных, получивших полную пренатальную подготовку соответственно современным подходам к лечению гестоза и профилактики его осложнений; 2-я подгруппа — 50 многорожавших беременных, получивших частичную дородовую подготовку в связи с поздней госпитализацией или началом родовой деятельности, что требовало ускорения родоразрешения. В группу сравнения вошли 50 многорожавших женщин без гестоза. Методы исследования. Клинико-статистический, калориметрический с помощью наборов коммерческих реактивов фирмы Boenringer Mannheim (ФРГ); плазменно-гематокритный (определение ОЦК), расчётный (определение ОЦП и ОЦЭ). Результаты исследования. Применение комплексной превентивной терапии у многорожавших женщин с гестозом способствовало наступлению клинической ремиссии у 82,7% беременных 1-й подгруппы. После проведённого лечения критические формы гестоза, диагностированные у 16% беременных 2-й подгруппы, отмечались лишь у 5,2% женщин из 1-й подгруппы. Однако, у пациенток с исходно тяжёлой нефропатией эффект имел временный характер, что свидетельствовало о глубоком нарушении защитно-приспособительных механизмов и выраженном катаболизме в организме многорожавших женщин с гестозом. Показатели ОЦК, ОЦП и ОЦЭ у многорожавших имеют тенденцию к снижению, особенно во 2-й подгруппе основной группы. Прогрессирование течения гестоза у многорожавших женщин сопровождается выраженным снижением уровня белков крови с относительным компенсаторным повышением концентраций — и, в меньшей степени, — глобулинов, свидетельствующие о напряжении метаболических процессов в организме. Гестоз у многорожавших женщин сопровождается нарушениями всех звеньев гемостаза по сравнению с группой многорожавших без гестоза. Наблюдается достоверное снижение числа тромбоцитов в венозной крови, повышение уровня гематокрита, укорочение времени свертывания и рекальцификации, отмечается возрастание ПТИ и уровня фибриногена. Заключение. На основании вышеизложенного можно сделать вывод об обратимости гемодинамических и гемостазиологических нарушений при правильном и своевременно начатом лечении гестоза лёгкой и средней степени тяжести. В то же время отсутствие эффекта от проводимой терапии указывает на стойкость и рефрактерность метаболических сдвигов при тяжёлых формах гестоза при многократных родах.

Association of COMT gene polymorphisms with Parkinson's disease

Parkinson’s disease (PD) is one of the most serious and widespread neurodegenerative disorders. Genetic susceptibility plays a significant role in the development of PD.The aim of our study was to investigate associations between single nucleotide polymorphisms (SNPs) of the COMT gene coding dopamine catabolism enzyme and Parkinson’s disease.Materials and methods. In this study seven SNPs (rs4680, rs6269, rs4633, rs4818, rs769224, rs165774, rs174696) of COMT were genotyped. 232 patients with PD and 127 healthy individuals in the Siberian region of Russia were examined. Venous blood samples were drawn as a marker of PD. Statistical differences in the prevalence of alleles and genotypes between groups of patients were assessed using IBM SPSS Statistics 23.1 software.Results. A significant association between the rs165774 polymorphism and PD was observed. Our study demonstrates that polymorphisms in the COMT gene may play an important role in the pathophysiology of Parkinson’s disease

Talaromyces atroroseus, a new species efficiently producing industrially relevant red pigments

Some species of Talaromyces secrete large amounts of red pigments. Literature has linked this character to species such as Talaromyces purpurogenus, T. albobiverticillius, T. marneffei, and T. minioluteus often under earlier Penicillium names. Isolates identified as T. purpurogenus have been reported to be interesting industrially and they can produce extracellular enzymes and red pigments, but they can also produce mycotoxins such as rubratoxin A and B and luteoskyrin. Production of mycotoxins limits the use of isolates of a particular species in biotechnology. Talaromyces atroroseus sp. nov., described in this study, produces the azaphilone biosynthetic families mitorubrins and Monascus pigments without any production of mycotoxins. Within the red pigment producing clade, T. atroroseus resolved in a distinct clade separate from all the other species in multigene phylogenies (ITS, β-tubulin and RPB1), which confirm its unique nature. Talaromyces atroroseus resembles T. purpurogenus and T. albobiverticillius in producing red diffusible pigments, but differs from the latter two species by the production of glauconic acid, purpuride and ZG-1494α and by the dull to dark green, thick walled ellipsoidal conidia produced. The type strain of Talaromyces atroroseus is CBS 133442

The Specific Features of the Course and Efficiency of Preventive Therapy for Gestosis in Multiple Labors

Objective: to study the course of gestosis in multiple labors and the impact of preventive therapy (a prospective study).Subjects. A hundred and fifty-eight females in the third trimester, including 108 multiparas with gestosis were examined (a study group). The study group was also divided in 2 subgroups: 1) 58 multipara pregnant females having a complete prenatal preparation in accordance with the current reasons for the treatment of gestosis and for the prevention of its complications; 2) 50 multipara pregnant females having a partial antenatal preparation due to late admission or to the onset of labor activity, which required accelerated delivery. A control group comprised 50 multiparas without gestosis.Methods. Clinical, statistical, and calorimetric studies using sets of commercial reagents (Boenringer Mannheim (Germany)); plasma hematocrit (determination of OTsK) and calculation (determination of OPTs and OTsE) methods.Results. Complex preventive therapy used in multiparas with gestosis promoted the occurrence of clinical remission in 82.7% of subgroup 1 pregnant females. After therapy, the critical forms of gestosis, diagnosed in 16% of subgroup 2 pregnant females, were observed in only 5.2% of subgroup 1 females. However, in patients with baseline severe nephropathy, the effect was temporary, which testified to a severe impairment of protective and adaptive mechanisms and to significant catabolism in multiparas with gestosis. The values of OTsK, OPTs, and OTsE in multiparas tended to decrease particularly in subgroup 2 of the study group. In multipara, progressive gestosis was accompanied by a pronounced decrease in the blood levels of proteins with a relatively compensatory rise in their concentration and, to a lesser extent, in that of globulins, suggesting the tension of metabolic processes in the body. Gestosis in multipara was attended by impairments in all hemostatic links, as compared to the control group. There was a significant decrease in the venous blood count of platelets, an increase in the level of hematocrit, and a reduction in the times of clotting and recalcification; there were also rises in the prothrombin index and fibrinogen levels.Conclusion. The findings lead to the conclusion that hemodynamic and hemostatic disorders are reversible when the treatment for mild and moderate gestosis is correct and is initiated in due time. At the same time, no benefit from the performed therapy testifies to the stability and refractoriness of metabolic shifts in severe gestosis during multiple labors

Phase composition and catalytic properties of MnNaW/SiO2 oxide system in oxidative conversion of methane

MnNaW/SiO2 oxide system based on a mesoporous silica matrix synthesized using tetraethoxysilane and cetyltrimethylammonium bromide as precursors were prepared and characterized by SEM/EDS, XRD, EPR, N-2 adsorption-desorption measurements and studied in the oxidative conversion of methane (OCM). It is shown that MnNaW/SiO2 catalyst consists of MnOx, Na2WO4, MnWO4, and SiO2 phases. At the reaction temperature of 750-850 degrees C the molten Na2WO4 phase covers the surface of crystalline SiO2, and the interaction of MnOx, Na2WO4 and SiO2 matrix forms "liquid glass". It is assumed that Na1-yMnOx particles formed as a result of the interaction of the system components during catalyst formation and characterized by the presence of ion-radical lattice oxygen are catalytically active sites in the OCM process

EPIDEMIOLOGICAL INDICATOR VALUE IN THE IODINE AVAILABILITY ASSESSMENT — EVIDENCE FROM THE REGIONS OF THE RUSSIAN FEDERATION

Background: In the Russian Federation, newborn screening comprises thyroid stimulating hormone determination to exclude primary congenital hypothyroidism. Screening is carried out throughout Russia. Neonatal TSH can be used to assess iodine deficiency and monitor iodine prevention programs.Objective: To assess and compare official statistical data on congenital hypothyroidism, the prevalence of hypothyroidism and iodine deficiency syndrome in children, as well as urinary iodine in the Russian regions.Materials and methods: The level of neonatal TSH was determined in 97.69% of children born in the Russian Federation in 2017. This article represents the results on the prevalence of hypothyroidism in the regions with various iodine availability. The correlation analysis was used to assess the relationship of CH incidence in newborns and iodine availability.Results: The calculated correlation coefficient, which was 0.2, reflects a weak relationship between the degree of iodine deficiency in the region and the number of newborns diagnosed with congenital hypothyroidism.Conclusions: In the Russian Federation, a law on universal salt iodization does not exist, and many regions are still in conditions of moderate or severe iodine deficiency. To assess the iodine status in these particular regions, we could use the results of newborn TSH screenin

CYP1A2 and CYP2D6 gene polymorphisms in schizophrenic patients with neuroleptic drug-induced side effects

Polymorphic variants of CYP1A2 and CYP2D6 genes of the cytochrome P450 system were studied in patients with schizophrenia with drug-induced motor disorders and hyperprolactinemia against the background of long-term neuroleptic therapy. We revealed an association of polymorphic variant C-163A CYP1A2*1F of CYP1A2 gene with tardive dyskinesia and association of polymorphic variant 1846G>A CY2D6*4 and genotype A/A of CYP2D6 gene (responsible for debrisoquin-4-hydroxylase synthesis) with limbotruncal tardive dyskinesia in patients with schizophrenia receiving neuroleptics for a long time