Comparative biology of common and grey seals along the Dutch coast : stranding, disease, rehabilitation and conservation

In Dutch waters, two seal species occur namely common seals (Phoca vitulina vitulina) and grey seals (Halichoerus grypus). In the 1970s, the common seal population reached a historical low point after centuries of hunting, followed by exposure to pollution. Grey seals had already disappeared from Dutch waters in the preceding centuries, probably due to hunting. In this thesis, seals were studied over a period of four decades (1971-2013) in which the common seals have shown a recovery and the grey seals successfully recolonised Dutch waters. The research is conducted in the period 2006-2013 at the Seal Rehabilitation and Research Centre (Pieterburen) in collaboration with several universities. A multidisciplinary approach has been applied to learn more about the biology and pathology of seals and to obtain a comprehensive view on their status in Dutch waters. The patterns and trends in stranding were studied for both seal species, followed by a study of their current genetic status. Based on the analysis of stranding data, parasitic pneumonia (common seals only) and orphanage (both species) were found to be the main causes of stranding. Therefore, the occurrence of parasitic infections and the breeding biology of seals were studied in the following chapters.Animal science

Clinical implications of the oncometabolite succinate in SDHx-mutation carriers

Succinate dehydrogenase (SDH) mutations lead to the accumulation of succinate, which acts as an oncometabolite. Germline SDHx mutations predispose to paraganglioma (PGL) and pheochromocytoma (PCC), as well as to renal cell carcinoma and gastro-intestinal stromal tumors. The SDHx genes were the first tumor suppressor genes discovered which encode for a mitochondrial enzyme, thereby supporting Otto Warburg's hypothesis in 1926 that a direct link existed between mitochondrial dysfunction and cancer. Accumulation of succinate is the hallmark of tumorigenesis in PGL and PCC. Succinate accumulation inhibits several α-ketoglutarate dioxygenases, thereby inducing the pseudohypoxia pathway and causing epigenetic changes. Moreover, SDH loss as a consequence of SDHx mutations can lead to reprogramming of cell metabolism. Metabolomics can be used as a diagnostic tool, as succinate and other metabolites can be measured in tumor tissue, plasma and urine with different techniques. Furthermore, these pathophysiological characteristics provide insight into therapeutic targets for metastatic disease. This review provides an overview of the pathophysiology and clinical implications of oncometabolite succinate in SDHx mutations

Eave tubes for malaria control in Africa: prototyping and evaluation against Anopheles gambiae s.s. and Anopheles arabiensis under semi-field conditions in western Kenya.

BACKGROUND: Whilst significant progress has been made in the fight against malaria, vector control continues to rely on just two insecticidal methods, i.e., indoor residual spraying and insecticidal bed nets. House improvement shows great potential to complement these methods and may further reduce indoor mosquito biting and disease transmission. Open eaves serve as important mosquito house entry points and provide a suitable location for intercepting host-seeking anophelines. This study describes semi-field experiments in western Kenya with eave tubes, a household protection product that leverages the natural behaviour of host-seeking malaria mosquitoes. METHODS: Semi-field experiments were conducted in two screen-houses. In both of these a typical western Kenyan house, with mud walls and corrugated iron sheet roofing, was built. Eave tubes with bendiocarb- or deltamethrin-treated eave tube inserts were installed in the houses, and the impact on house entry of local strains of Anopheles gambiae and Anopheles arabiensis was determined. Experiments with open eave tubes (no netting) were conducted as a control and to determine house entry through eave tubes. Insecticidal activity of the inserts treated with insecticide was examined using standard 3-min exposure bioassays. RESULTS: Experiments with open eave tubes showed that a high percentage of released mosquitoes entered the house through tubes during experimental nights. When tubes were fitted with bendiocarb- or deltamethrin-treated inserts, on average 21% [95% CI 18-25%] and 39% [CI 26-51%] of An. gambiae s.s. were recaptured the following morning, respectively. This contrasts with 71% [CI 60-81%] in the treatment with open eaves and 54% [CI 47-61%] in the treatment where inserts were treated with fluorescent dye powder. For An. arabiensis recapture was 21% [CI 14-27%] and 22% [CI 18-25%], respectively, compared to 46% [CI 40-52%] and 25% [CI 15-35%] in the treatments with open tubes and fluorescent dye. CONCLUSIONS: Insecticide-treated eave tubes resulted in significant reductions in recapture rates for both malaria vector species, representing the first and promising results with this novel control tool against Kenyan malaria vectors. Further field evaluation of eave tubes under more realistic field conditions, as well as their comparison with existing approaches in terms of cost-effectiveness and community acceptance, is called for

Machine learning for regional crop yield forecasting in Europe

Crop yield forecasting at national level relies on predictors aggregated from smaller spatial units to larger ones according to harvested crop areas. Such crop areas come from land cover maps or reported statistics, both of which can have errors and uncertainties. Sub-national or regional crop yield forecasting minimizes the propagation of these errors to some extent. In addition, regional forecasts provide added value and insights to stakeholders on regional differences within a country, which would otherwise compensate each other at national level. We propose a crop yield forecasting approach for multiple spatial levels based on regional crop yield forecasts from machine learning. Machine learning, with its data-driven approach, can leverage larger data sizes and capture nonlinear relationships between predictors and yield at regional level. We designed a generic machine learning workflow to demonstrate the benefits of regional crop yield forecasting in Europe. To evaluate the quality and usefulness of regional forecasts, we predicted crop yields for 35 case studies, including nine countries that are major producers of six crops (soft wheat, spring barley, sunflower, grain maize, sugar beets and potatoes). Machine learning models at regional level had lower normalized root mean squared errors (NRMSE) and uncertainty than a linear trend model, with Wilcoxon p-values of 3e-7 and 2e-7 for 60 days before harvest and end of season respectively. Similarly, regional machine learning forecasts aggregated to national level had lower NRMSEs than forecasts from an operational system in 18 out of 35 cases 60 days before harvest, with a Wilcoxon p-value of 0.95 indicating similar performance. Our models have room for improvement, especially during extreme years. Nevertheless, regional crop yield forecasts from machine learning and aggregated national forecasts provide a consistent forecasting method across spatial levels and insights from regional differences to support important policy decisions

De overgang van primair naar voortgezet onderwijs in internationaal perspectief:Een systematische overzichtsstudie van onderwijstransities in relatie tot kenmerken van verschillende Europese onderwijsstelsels.

NRO-OPRO project 405-15-72

Unilateral and Bilateral Adrenalectomy for Pheochromocytoma Requires Adjustment of Urinary and Plasma Metanephrine Reference Ranges

Context: Follow-up after adrenalectomy for pheochromocytoma is recommended because of a recurrence risk. During follow-up, plasma and/or urinary metanephrine (MN) and normetanephrine (NMN) are interpreted using reference ranges obtained in healthy subjects. Objective: Because adrenalectomy may decrease epinephrine production, we compared MN and NMN concentrations in patients after adrenalectomy to concentrations in a healthy reference population. Design: A single-center cohort study was performed in pheochromocytoma patients after adrenalectomy between 1980 and 2011. Subjects: Seventy patients after unilateral and 24 after bilateral adrenalectomy were included. Main Outcome Measures: Plasma-free and urinary-deconjugated MN and NMN determined at 3 to 6 months and annually until 5 years after adrenalectomy were compared with concentrations in a reference population. Data are presented in median (interquartile range). Results: Urinary and plasma MN concentrations 3 to 6 months after unilateral adrenalectomy were lower compared with the reference population (39 [31-53] mu mol/mol creatinine and 0.14 [0.09-0.18] nmol/L vs 61 [49-74] mu mol/mol creatinine and 0.18 [0.13-0.23] nmol/L, respectively, both P <.05). Urinary MN after bilateral adrenalectomy was reduced even further (7 [1-22] mu mol/mol creatinine; P <.05). Urinary and plasma NMN were higher after unilateral adrenalectomy (151 [117-189] mu mol/mol creatinine and 0.78 [0.59-1.00] nmol/L vs 114 [98-176] mu mol/mol creatinine and 0.53 [0.41-0.70] nmol/L; both P <.05). Urinary NMN after bilateral adrenalectomy was higher (177 [106-238] mu mol/mol creatinine; P <.05). Changes in urinary and plasma MNs persisted during follow-up. Conclusion: Concentrations of MN are decreased, whereas NMN concentrations are increased after unilateral and bilateral adrenalectomy. Adjusted reference values for MN and NMN are needed in the postsurgical follow-up of pheochromocytoma patients. (J Clin Endocrinol Metab 98:1076-1083, 2013

A MeerKAT-meets-LOFAR study of Abell 1413: a moderately disturbed non-cool-core cluster hosting a ∼500\sim 500 kpc 'mini'-halo

Many relaxed cool-core clusters host diffuse radio emission on scales of hundreds of kiloparsecs: mini-haloes. However, the mechanism responsible for generating them, as well as their connection with central active galactic nuclei, is elusive and many questions related to their physical properties and origins remain unanswered. This paper presents new radio observations of the galaxy cluster Abell 1413 performed with MeerKAT (L-band; 872 to 1712 MHz) and LOFAR HBA (120 to 168 MHz) as part of a statistical and homogeneous census of mini-haloes. Abell 1413 is unique among mini-halo clusters as it is a moderately-disturbed non-cool-core cluster. Our study reveals an asymmetric mini-halo up to 584 kpc in size at 1283 MHz, twice as large as first reported at similar frequencies. The spectral index is flatter than previously reported, with an integrated value of $\alpha = -1.01 \pm 0.06$, shows significant spatial variation, and a tentative radial steepening. We studied the point-to-point X-ray/radio surface brightness correlation to investigate the thermal/non-thermal connection: our results show a strong connection between these components, with a super-linear slope of $b = 1.63 \pm 0.10$ at 1283 MHz and $b = 1.20 \pm 0.12$ at 145 MHz. We also explore the X-ray surface brightness/radio spectral index correlation, finding a slope of $b = 0.59 \pm 0.11$. Both investigations support the evidence of spectral steepening. Finally, in the context of understanding the particle acceleration mechanism, we present a simple theoretical model which demonstrates that hybrid scenarios - secondary electrons (re-)accelerated by turbulence - reproduce a super-linear correlation slope.Comment: 19 pages, 11 figures, 4 tables. Accepted for publication in MNRA

Novel B19-like parvovirus in the brain of a harbor seal

Using random PCR in combination with next-generation sequencing, a novel parvovirus was detected in the brain of a young harbor seal (Phoca vitulina) with chronic non-suppurative meningo-encephalitis that was rehabilitated at the Seal Rehabilitation and Research Centre (SRRC) in the Netherlands. In addition, two novel viruses belonging to the family Anelloviridae were detected in the lungs of this animal. Phylogenetic analysis of the coding sequence of the novel parvovirus, tentatively called Seal parvovirus, indicated that this virus belonged to the genus Erythrovirus , to which human parvovirus B19 also belongs. Although no other seals with similar signs were rehabilitated in SRRC in recent years, a prevalence study of tissues of seals from the same area collected in the period 2008-2012 indicated that the Seal parvovirus has circulated in the