Generation and Analysis of Expressed Sequence Tags from Olea europaea L.

Olive (Olea europaea L.) is an important source of edible oil which was originated in Near-East region. In this study, two cDNA libraries were constructed from young olive leaves and immature olive fruits for generation of ESTs to discover the novel genes and search the function of unknown genes of olive. The randomly selected 3840 colonies were sequenced for EST collection from both libraries. Readable 2228 sequences for olive leaf and 1506 sequences for olive fruit were assembled into 205 and 69 contigs, respectively, whereas 2478 were singletons. Putative functions of all 2752 differentially expressed unique sequences were designated by gene homology based on BLAST and annotated using BLAST2GO. While 1339 ESTs show no homology to the database, 2024 ESTs have homology (under 80%) with hypothetical proteins, putative proteins, expressed proteins, and unknown proteins in NCBI-GenBank. 635 EST's unique genes sequence have been identified by over 80% homology to known function in other species which were not previously described in Olea family. Only 3.1% of total EST's was shown similarity with olive database existing in NCBI. This generated EST's data and consensus sequences were submitted to NCBI as valuable source for functional genome studies of olive

Awareness of medical and aromatic plants in the western black sea region

In our research, the level of awareness about medicinal and aromatic plants in different professions groups (farmers, civilservants, workers, retired, students and unemployed) and education levels were examined through direct interviews inWestern Black Sea Region (Düzce, Sakarya, Zonguldak, Bartın). By using the Neyman method, 77 people wereinvestigated, based on the Likert scale to measure awareness levels, and the results were evaluated by SPSS method.As a result of our research, 24.7% of them prefer medicinal and aromatic plants when they are sick, while 81.8% thinkthat medicinal and aromatic plants are organic. 66.2 %agree that organic certification is required for medicinal andaromatic plants. 63.6% of inadequate education is remarkable when the problems are expressed. At the rate of 5.2% is TVand 15.6% is for newspapers are used for information acquisition. According to the study, it was concluded that the levelof awareness of the medicinal and aromatic plants in the Western Black Sea region is not sufficient, and starting fromschool programs, it is necessary to raise awareness of the public by means of non-formal education, magazines andcourses besides TVand newspaper

Berry Fruits Grown in Duzce and Its Neighborhood: Their Medical Applications

WOS: 000418549100003Berry fruits possess many positive effects on human health because of their phytochemical contents like phenolic compounds, antioxidants and anthocyanins, and they have also an important place among the functional foods. From the varieties of Isabella grape and Bursa-2 blackberry cultivated in Duzce and its neighborhood, aging-retardant cream, vinegar (cyme) which aids in the weight loss and alleviates the stomach discomfort, and grape and blackberry juice are produced. Besides, other berry fruits are also used but only in the form of folk medicine. Thus, further studies about the berry fruits grown in the region may increase the potential medical usages of those plants

Subjective Effects of Lemon Seed, Rose and Lavender Essential Oils onHumans: A Case Study from Two Different Age Groups

Plant volatile oils have been used for different purposes for years, especially in the scientific and commercial areas. Among the major areas of their uses were the cosmetic, pharmaceutical and food industries, and the aroma/phyto therapeutic applications. The lemon seed, rose and lavender oils were the most commonly used oils and their effects on humans also differed individually. In this study, effects of these essential (volatile) oils on two age groups, 17-24 and 25-40 were thus investigated. The study was conducted on 80 people for one hour and the effects were evaluated using questionnaires. We used SPSS method for statics. In 17-25 age group, lavender oil caused drowsiness and weakness in 60% and 50% of respondents respectively, and also some mild giddiness, nausea, headache and dizziness. In 25-40 age group it caused giddiness and weakness in 40% respondents, and also gave mild vigor and tranquility. Lemon seed oil caused drowsiness in 85%, weakness and headache in 50%, and clear sensation in 55% of the respondents from 17-24 age group, while in 25-40 age group it caused clear sensation in 80% of respondents, and gave the mood of tranquility in 70% and happiness in 60%. However, rose oil demonstrated more intense effects on people, causing drowsiness and nausea in 55%, weakness and headache in 50%, and also giddiness, face flush and palpitation in 17-24 age-group-respondents. In 25-40 age group it caused drowsiness and nausea in 40% while it gave happiness and tranquility in 60% of respondents. Study results indicated that effects of different types of volatile oils on humans vary based on the age groups. So, the preference of essential oils in our daily lives is implied to have great importance. Therefore, further studies on the subject should be conducted to better emphasize the choice and use of these oils based on the age groups

Visual Evoked Potential Changes In Chronic Obstructive Pulmonary Disease

Aim: The normal visual evoked potential (VEP) reflects the functional integrity of the visual pathways from retinal to occipital striate area. Visual reseptors are sensitive to hypoxemia. Chronic obstructive pulmonary disease (COPD) is a progressive disease, in which hypoxemia occurs due to ventilation-perfusion imbalance. We aimed to evaluate the effects of COPD on VEPs. Methods: Thirty eight COPD patients and 17 healthy control subjects were accepted to the study. Pulmonary function tests were performed to all the participants. VEP of all participants were recorded. Results: P1 (P100) values of both right and left eyes (p=0.008 and p=0.010, respectively) and N2 value of right eye (p=0.030) were significantly higher in COPD patients than the control group. As there was just 1 female COPD patient, the measurements were re-evaluated for just male subjects. P1 values of both right and left eyes were significantly higher in male COPD patients than male control subjects (p=0.031 and p=0.023, respectively). Conclusion: VEPs, particularly P1 value, alters in COPD patients. This change in VEPs was thought to be due to hypoxemia caused by ventilation-perfusion imbalance in COPD

COVID-19 Pandemi sürecinde Tüberküloz Tanısı Alan Hastaların Deneyimleri: Nitel Araştırma

Amaç: Bu araştırma, COVID-19 pandemi sürecinde tüberküloz tanısı alan hastaların yaşadıkları deneyimlerin belirlenmesi amacıyla yürütülmüştür. Yöntem: Nitel bir araştırma olarak yapılan araştırmada, Samsun İlkadım Verem Savaş Dispanseri’ne kayıtlı 14 tüberküloz hastası ile 19 Kasım-15 Aralık 2021 tarihleri arasında yüz yüze görüşmeler yapılmıştır. Görüşmeler esnasında, yarı yapılandırılmış görüşme formu kullanılmıştır. Elde edilen veriler tümevarımsal tematik analiz prosedürüne göre analiz edilmiştir. Bulgular: Bu araştırmada iki ana tema belirlenmiştir. Bunlar; bireysel tepkiler ve baş etme temalarıdır. Bu temalar, alt temalara ayrılmıştır. Bireysel tepkiler temasının alt temaları emosyonel, fizyolojik ve davranışsal tepkiler; baş etme temasının alt temaları dini baş etme ve kabullenmedir. Sonuç: COVID-19 pandemi sürecinde tüberküloz hastalarının tanı aldıklarında emosyonel, fizyolojik ve davranışsal olarak bireysel tepkiler gösterdikleri, dini baş etme ve kabullenme yöntemleri ile bu tepkilerle baş ettikleri belirlenmiştir. Tüberküloz hastalarında fizyolojik, emosyonel ve davranışsal tepkilerin azaltılması ve bu tepkilerle etkili baş etme tekniklerinin öğretilmesi için hastalara tüberkülozun semptomları, semptom yönetimi, tedavi süreci, ilaçlar ve stresle baş etme teknikleri konularında eğitim verilmesi önerilmektedir

Assessment of genetic variations of silver lime (Tilia tomentosa Moench.) by RAPD markers in urban and forest ecosystems

Filiz, Ertugrul/0000-0001-9636-6389; Ozyigit, Ibrahim Ilker/0000-0002-0825-5951WOS: 000356960300004In the present study, the genetic diversity analysis of Tilia tomentosa plants was performed by using random amplified polymorphic DNA (RAPD) primers. Twenty eight plant samples, collected from urban (25 members) and forest (3 members) ecosystems, were used in this study. A total of 53 bands were obtained from eight RAPD primers, of which 48 (90.6%) were polymorphic. The percentage of polymorphic loci (P) was found to be 94.29%, the observed number of alleles (Na) was 1.94, the effective number of alleles (Ne) was 1.60, Nei's gene diversity (h) was 0.34 and Shannon's information index (I) was 0.50. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis revealed two major groups. Members of the urban and forest ecosystems showed high genetic similarity (28%-92%) and they did not separate from each other in UPGMA tree. Furthermore, urban and forest genotypes clustered together in principal component analysis.Scientific Research Projects of Duzce UniversityDuzce University [2012.23.01.116]; Marmara UniversityMarmara University [FEN-D-140115-0014]Scientific Research Projects of Duzce University [grant number 2012.23.01.116]; Marmara University [grant number FEN-D-140115-0014]