387 research outputs found
The Near-Horizon Limit of the Extreme Rotating d=5 Black Hole as a Homogenous Spacetime
We show that the spacetime of the near-horizon limit of the extreme rotating
d=5 black hole, which is maximally supersymmetric in N=2,d=5 supergravity for
any value of the rotation parameter j in [-1,1], is locally isomorphic to a
homogeneous non-symmetric spacetime corresponding to an element of the
1-parameter family of coset spaces SO(2,1)x SO(3)/SO(2)_j in which the subgroup
SO(2)_j is a combination of the two SO(2) subgroups of SO(2,1) and SO(3).Comment: Some points clarified and misprints corrected. Version to be
published in Classical and Quantum Gravit
On d=4,5,6 Vacua with 8 Supercharges
We show how all known N=2, d=4,5,6 maximally supersymmetric vacua (Hpp-waves
and aDSxS solutions) are related through dimensional reduction/oxidation
preserving all the unbroken supersymmetries. In particular we show how the N=2,
d=5 family of vacua (which are the near-horizon geometry of supersymmetric
rotating black holes) interpolates between aDS_2xS^3 and aDS_3xS^2 in parameter
space and how it can be dimensionally reduced to an N=2, d=4 dyonic
Robinson-Bertotti solution with geometry aDS_2xS^2 and oxidized to an N=2, d=6
solution with aDS_3xS^3 geometry (which is the near-horizon of the self-dual
string).Comment: Latex2e, 19 pages, 1 figure. v2: typos corrected, refs. added. v3:
very minor corrections, more refs. added, version to be published in
Classical and Quantum Gravit
Anisotropy of magnetic susceptibility analysis of the Cantera Ignimbrite, San Luis Potosi, México: flow source recognition
Geometric Construction of Killing Spinors and Supersymmetry Algebras in Homogeneous Spacetimes
We show how the Killing spinors of some maximally supersymmetric supergravity
solutions whose metrics describe symmetric spacetimes (including and H-waves) can be easily constructed using purely geometrical and
group-theoretical methods. The calculation of the supersymmetry algebras is
extremely simple in this formalism.Comment: misprints corrected and references added. Version to appear in
Classical and Quantum Gravit
Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application
Background
The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have developed a complete and original process for the production and recovery of the properly folded intracellular domain of IA-2 fused to thioredoxin (TrxIA-2ic) in Escherichia coli GI698 and GI724 strains. We have also carried out the biochemical and immunochemical characterization of TrxIA-2icand design variants of non-radiometric immunoassays for the efficient detection of IA-2 autoantibodies (IA-2A).
Results
The main findings can be summarized in the following statements: i) TrxIA-2ic expression after 3 h of induction on GI724 strain yielded ≈ 10 mg of highly pure TrxIA-2ic/L of culture medium by a single step purification by affinity chromatography, ii) the molecular weight of TrxIA-2ic (55,358 Da) could be estimated by SDS-PAGE, size exclusion chromatography and mass spectrometry, iii) TrxIA-2ic was properly identified by western blot and mass spectrometric analysis of proteolytic digestions (63.25 % total coverage), iv) excellent immunochemical behavior of properly folded full TrxIA-2ic was legitimized by inhibition or displacement of [35S]IA-2 binding from IA-2A present in Argentinian Type 1 Diabetic patients, v) great stability over time was found under proper storage conditions and vi) low cost and environmentally harmless ELISA methods for IA-2A assessment were developed, with colorimetric or chemiluminescent detection.
Conclusions
E. coli GI724 strain emerged as a handy source of recombinant IA-2ic, achieving high levels of expression as a thioredoxin fusion protein, adequately validated and applicable to the development of innovative and cost-effective immunoassays for IA-2A detection in most laboratories.Fil: Guerra, Luciano Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Faccinetti, Natalia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Trabucchi, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Rovitto, Bruno David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Sabljic, Adriana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Poskus, Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Iacono, Ruben Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Valdez, Silvina Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentin
Does Accessibility to Higher Education Matter? Choice Behavior of High School Graduates in the Netherlands
Moving Targets, Long-Lived Infrastructure, and Increasing Needs for Integration and Adaptation in Water Management: An Illustration from Switzerland
About Attitudes and Perceptions: Finding the Proper Way to Consider Latent Variables in Discrete Choice Models
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