Low appendicular muscle mass is correlated with femoral neck bone mineral density loss in postmenopausal women

<p>Abstract</p> <p>Background</p> <p>After menopause, rapid bone mass loss occurs in response to hypoestrogenism. Several studies suggest that muscle mass and bone mineral density (BMD) are positively associated in postmenopausal women. Therefore, it may be assumed that postmenopausal low appendicular muscle mass (aMM) can increase BMD loss in a short period of time.</p> <p>Objective</p> <p>The purpose of this study was to assess relationship of aMM with femoral neck BMD in postmenopausal women.</p> <p>Methods</p> <p>Prospective, controlled clinical Trial including 64 women aged 45-70 years, who had not had their last menstruation for at least one year. Subjects were divided into two groups: low aMM (n = 32), and normal aMM (n-32). Femoral neck BMD and muscle mass were measured by DXA at baseline and after twelve months. Pairwise and independent t tests were used for data analysis.</p> <p>Results</p> <p>Baseline weight, BMI and muscle mass (total and appendicular) significantly differ between groups (p < 0.05). After twelve months, femoral neck BMD was significantly lower in the group with low aMM, whereas no significant difference was observed in the group with normal aMM (p < 0.05).</p> <p>Conclusion</p> <p>In postmenopausal women, low appendicular muscle mass is associated negatively with femoral neck BMD in a short period of time.</p

IDENTIFICAZIONE DELLE LESIONI POLMONARI IN RM CON UNA SEQUENZA VIBE MODIFICATA E CON UNA SEQUENZA VIBE STANDARD: UN CONFRONTO CON LA TC.

Scopo valutare l\u2019accuratezza della RM a 3 Tesla nell\u2019individuazione di lesioni polmonari con una sequenza VIBE modificata e con una sequenza VIBE standard, usando la TC come riferimento. Materiale e metodi abbiamo rivalutato retrospettivamente 37 pazienti oncologici (11 pediatrici e 26 adulti; 19 F) sottoposti a TC e PET/RM da 3T il cui protocollo prevedeva una sequenza VIBE acquisita a respiro trattenuto con echo time (TE) di 0.89 ms e flip angle (FA) di 3\ub0. Le VIBE sono state valutate da tre osservatori per l'identificazione di noduli >5mm o =5mm. Un quarto osservatore ha valutato delle sequenze VIBE standard (TE di 1.2 ms e FA di 10\ub0), anch\u2019esse incluse nel protocollo, ed infine un quinto lettore ha analizzato le immagini TC (considerate come standard di riferimento).Sia per paziente che per singola lesione sono state calcolate sensibilit\ue0 e specificit\ue0 per le due categorie di noduli ed il coefficiente di correlazione intraclasse (ICC) per i lettori delle VIBE modificate. Risultati analisi per paziente (positivit\ue0=1 lesione): sensibilit\ue0 84.6% e specificit\ue0 di 100% per noduli>5mm (VIBE standard 69.2% e 100%) e 44.4% e 100% per noduli=5mm (VIBE standard 33.3% e 100%). Analisi per lesione (presenza/assenza): sensibilit\ue0 di 83.9% per noduli>5mm (VIBE standard 67.74%) e 37.5% per noduli=5mm (VIBE standard 18.7%). L\u2019ICC nell\u2019analisi per paziente era 0.911 per noduli>5mm e 0.902 per noduli=5mm; nell\u2019analisi per lesione 0.866 per noduli>5mm e 0.699 per noduli=5mm. Conclusioni la sequenza VIBE modificata \ue8 riproducibile ed accurata per l\u2019individuazione di noduli >5mm, mentre l\u2019accuratezza risulta meno soddisfacente per i noduli=5mm. L\u2019utilizzo della sequenza modificata nei protocolli RM sembra ragionevole per migliorare la visualizzazione del polmone

Broad-band Optical Polarimetric Studies toward the Galactic young star cluster Be 59

We present multiwavelength optical linear polarimetric observations of 69 stars toward the young open cluster Be 59. The observations reveal the presence of three dust layers located at the distances of \sim300, \sim500 and \sim700 pc. The dust layers produce a total polarization Pv \sim 5.5 per cent. The mean values of polarization and polarization angles due to the dust layers are found to increase systematically with distance. We show that polarimetry in combination with the (U - B) - (B - V) colour-colour diagram yields a better identification of cluster members. The polarization measurements suggest that the polarization due the intra-cluster medium is \sim 2.2 per cent. An anomalous reddening law exists for the cluster region, indicating a relatively larger grain size than that in the diffuse ISM. The spatial variation of the polarization and E(B - V) is found to increase with radial distance from the cluster center, whereas the {\theta}v and {\lambda}max are found to decrease with increasing radial distance from the cluster center. About 40 per cent of cluster members show the signatures of either intrinsic polarization or rotation in their polarization angles. There is an indication that the star light of the cluster members might have been depolarized because of non-uniform alignment of dust grains in the foreground dust layers and in the intra-cluster medium.Comment: 22 pages, 14 figures and 7 tables, Accepted in MNRA

Hepatitis C virus NS5A is a direct substrate of casein kinase I-&#945;, a cellular kinase identified by inhibitor affinity chromatography using specific NS5A hyperphosphorylation inhibitors

The hepatitis C virus encodes a single polyprotein that is processed by host and viral proteases to yield at least 10 mature viral proteins. The nonstructural (NS) protein 5A is a phosphoprotein, and experimental data indicate that the phosphorylation state of NS5A is important for the outcome of viral RNA replication. We were able to identify kinase inhibitors that specifically inhibit the formation of the hyperphosphorylated form of NS5A (p58) in cells. These kinase inhibitors were used for inhibitor affinity chromatography in order to identify the cellular targets of these compounds. The kinases casein kinase I (CKI), p38 MAPK, CIT (Citron Rho-interacting kinase), GAK, JNK2, PKA, RSK1/2, and RIPK2 were identified in the high affinity binding fractions of two NS5A hyperphosphorylation inhibitors (NS5A-p58-i). Even though these kinases are targets of the NS5A-p58-i, the only kinase showing an effect on NS5A hyperphosphorylation was confirmed to be CKI-\u3b1. Although this finding does not exclude the possibility that other kinase(s) might be involved in basal or regulatory phosphorylation of NS5A, we show here that NS5A is a direct substrate of CKI-\u3b1. Moreover, in vitro phosphorylation of NS5A by CKI-\u3b1 resulted for the first time in the production of basal and hyperphosphorylated forms resembling those produced in cells. In vitro kinase reactions performed with NS5A peptides show that Ser-2204 is a preferred substrate residue for CKI-\u3b1 after pre-phosphorylation of Ser-2201

Multi-wavelength polarimetric study towards the open cluster NGC 1893

We present multi-wavelength linear polarimetric observations for 44 stars of the NGC 1893 young open cluster region along with V-band polarimetric observations of stars of other four open clusters located between l ~160 to ~175 degree. We found evidence for the presence of two dust layers located at a distance of ~170 pc and ~360 pc. The dust layers produce a polarization Pv ~2.2%. It is evident from the clusters studied in the present work that, in the Galactic longitude range l ~160 to 175 degree and within the Galactic plane (|b| < 2 degree), the polarization angles remain almost constant, with a mean ~163 degree and a dispersion of 6 degree. The small dispersion in polarization angle could be due to the presence of uniform dust layer beyond 1 kpc. Present observations reveal that in case of NGC 1893, the foreground two dust layers, in addition to the intracluster medium, seems to be responsible for the polarization effects. It is also found that towards the direction of NGC 1893, the dust layer that exists between 2-3 kpc has a negligible contribution towards the total observed polarization. The weighted mean for percentage of polarization (Pmax) and the wavelength at maximum polarization ({\lambda}max) are found to be 2.59 \pm 0.02% and 0.55 \pm 0.01 \mum respectively. The estimated mean value of {\lambda}max indicates that the average size of the dust grains within the cluster is similar to that in the general interstellar medium. The spatial variation of the polarization is found to decrease towards the outer region of the cluster. In the present work, we support the notion, as already has been shown in previous studies, that polarimetry, in combination with (U-B)/(B-V) colour-colour diagram, is a useful tool for identifying non-members in a cluster.Comment: 36 pages, 12 figures, 10 tables, accepted for the publication in MNRA

The Web will kill them all: new media, digital utopia, and political struggle in the Italian 5-Star Movement

This article examines the role of discourses about new media technology and the Web in the rise of the 5-Star Movement (Movimento 5 Stelle, or M5S) in Italy. Founded by comedian and activist Beppe Grillo and Web entrepreneur Gianrobe rto Casaleggio in 2009, this movement succeeded in becoming the second largest party at t he 2013 national elections in Italy. This article aims to discuss how elements of digital uto pia and Web-centric discourses have been inserted into the movement’s political message, and how the construction of the Web as a myth has shaped the movement’s discourse and political p ractice. The 5-Star Movement is compared and contrasted with other social and political move ments in Western countries which have displayed a similar emphasis on new media, such as the Occupy movement, the Indignados movement, and the Pirate Parties in Sweden and Germ any. By adopting and mutating cyber- utopian discourses from the so-called Californian i deology, the movement symbolically identifies itself with the Web. The traditional political esta blishment is associated with “old” media (television, radio, and the printed press), and rep resented as a “walking dead,” doomed to be superseded and buried by a Web-based direct democra cy

Associations between healthy eating patterns and indicators of metabolic risk in postmenopausal women

<p>Abstract</p> <p>Background</p> <p>Since human diets contain many components that may work synergistically to prevent or promote disease, assessing diet quality may be informative. The purpose of this study was to investigate the association between quality diet, by using Healthy Eating Index (HEI), and metabolic risk indicators in postmenopausal women.</p> <p>Methods</p> <p>This cross-sectional study included a total of 173 Brazilian women, aged 45-75 years, seeking healthcare at a public outpatient center. Food consumption assessed by 24 h-recall food inquiry was used to calculate HEI scores: >80 implied diet good, 80-51 diet "needed improvement", and <51 diet poor. Anthropometric data included: body mass index (BMI = weight/height²), waist-circumference (WC), body fat (%BF) and lean mass (%LM). Data on total cholesterol (TC), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), and triglycerides (TG) were also collected. Fisher's Exact test, and logistic regression method (to determine odds ratio, OR) were used in the statistical analysis.</p> <p>Results</p> <p>Overweight and obesity were observed in 75.7% of the participants. Excessive %BF (> 35%) was observed in 56.1%, while %LM was reduced (< 70%) in 78.1%. WC was elevated (≥88 cm) in 72.3%. Based on HEI values, diet quality was good in 3% (5/173), needed improvement in 48.5% (84/173), and was poor in 48.5% (84/173) of the cases. In this group, 75% of women had high intakes of lipids (> 35%), predominantly saturated and monounsaturated fat. On average, plasma TC, LDLC, and TG levels were higher than recommended in 57.2%, 79.2% and 45.1% of the women, respectively, while HDLC was low in 50.8%. There was association between HEI scores and the %BF that it was higher among women with HEI score < 80 (p = 0.021). There were not observed significant risk associations between HEI and lipid profile.</p> <p>Conclusion</p> <p>Among the Brazilian postmenopausal women attending a public outpatient clinic, diet was considered to need improvement or to be of poor quality, attributed to high saturated fat ingestion, which probably caused a negative impact on metabolic risk indicators, namely body composition.</p

Dietary factors associated with metabolic syndrome in Brazilian adults

<p>Abstract</p> <p>Background</p> <p>Metabolic Syndrome (MS) is defined as the association of numerous factors that increase cardiovascular risk and diet is one of the main factors related to increase the MS in the population. This study aimed to evaluate the association of diet on the presence of MS in an adult population sample.</p> <p>Methodology</p> <p>305 adults were clinically screened to participate in a lifestyle modification program. Anthropometric assessments included waist circumference (WC), body fat and calculated BMI (kg/m²) and muscle-mass index (MMI kg/m²). Dietary intake was estimated by 24 h dietary recall. Fasting blood was used for biochemical analysis. MS was diagnosed using NCEP-ATPIII (2001) criteria with adaptation for glucose (≥ 100 mg/dL). Logistic regression (Odds ratio) was performed in order to determine the odds ratio for developing MS according to dietary intake.</p> <p>Results</p> <p>An adequate intake of fruits, OR = 0.52 (CI:0.28-0.98), and an intake of more than 8 different items in the diet (variety), OR = 0.31 (CI:0.12-0.79) showed to be a protective factor against a diagnosis of MS. Saturated fat intake greater than 10% of total caloric value represented a risk for MS diagnosis, OR = 2.0 (1.04-3.84).</p> <p>Conclusion</p> <p>Regarding the dietary aspect, a risk factor for MS was higher intake of saturated fat, and protective factors were high diet variety and adequate fruit intake.</p