Contra a ontologia incorpórea heideggeriana : ensaiando uma ontologia estético-existencial com Oswald de Andrade, Vilém Flusser e Marcia Tiburi

Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Humanas, Programa de Pós-Graduação em Filosofia, 2013.Este texto acompanha a ontologia-existencial heideggeriana de perto, principalmente a virada poética desta ontologia, no que esta permite criticar e radicalizar ontologias tradicionais, incluindo estéticas tradicionais, com seus pressupostos metafísicos, ora transcendentes ora transcendentais. Não obstante, passo a questionar a ontologia incorpórea heideggeriana através da corporalidade devoradora (Oswald de Andrade), da corporalidade apátrida (Vilém Flusser) e da corporalidade cinza (Marcia Tiburi), ensaiando uma ontologia estético-existencial. ______________________________________________________________________________ ABSTRACTThe present text follows the Heideggerian existential ontology very closely, in particular in its poetic turning point opening the possibility of making criticisms and to radicalize traditional ontologies and aesthetics with their metaphysical assumptions, transcendent or transcendental. But I contest nevertheless the incorporeal Heideggerian ontology by means of a devoring (Oswald de Andrade), stateless (Vilém Flusser) and grey (Marcia Tiburi) corporeality aiming at the formulation of some Existential- aesthetic ontology

Le corps signifiant : Hendrix, le 31 août 1970, 02h00

À la fin de son interprétation de « Foxy Lady » à l’Île de Wight, Jimi Hendrix fait jaillir des glissandos de sa guitare, grimace et laisse tomber sa tête en arrière, comme s’il cherchait à imiter une poupée de chiffon, avant de conclure en effectuant le salut caractéristique d’un véritable showman. Quel sens donner à ce jeu de scène extravagant ? Telle est la question à laquelle Nicholas Cook a tenté de répondre à l’occasion d’une conférence organisée en Sorbonne dans le cadre des Rencontres..

Phylogenetic And Molecular Variability Studies Reveal A New Genetic Clade Of Citrus Leprosis Virus C

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Citrus leprosis virus C (CiLV-C) causes a severe disease affecting citrus orchards in the Western hemisphere. This study reveals the molecular variability of the virus by analyzing four genomic regions (p29, p15, MP and RNA2-intergenic region) distributed over its two RNAs. Nucleotide diversity (pi) values were relatively low but statistically different over the analyzed genes and subpopulations, indicating their distinct evolutionary history. Values of pi(p29) and pi(MP) were higher than those of pi(p15) and pi(RNA2-IR), whereas pi(MP) was increased due to novel discovered isolates phylogenetically clustered in a divergent clade that we called SJP. Isolate BR_SP_SJP_01 RNA1 and RNA2 sequences, clade SJP, showed an identity of 85.6% and 88.4%, respectively, with those corresponding to CiLV-C, the type member of the genus Cilevirus, and its RNA2 5'-proximal region was revealed as a minor donor in a putative inter-clade recombination event. In addition to citrus, BR_SP_SJP_01 naturally infects the weed Commelina benghalensis and is efficiently transmitted by Brevipalpus yothersi mites. Our data demonstrated that negative selection was the major force operating in the evaluated viral coding regions and defined amino acids putatively relevant for the biological function of cilevirus proteins. This work provides molecular tools and sets up a framework for further epidemiological studies.86Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [375843/2012-4, 481771/2013-1, 401564/2012-6]Fundacao de Apoio a Pesquisa no Estado de Sao Paulo [2012/18771-0, 2012/20667-7, 2014/08458-9]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Molecular cloning of stress-induced genes of maize (Zea mays L.) using the pcr-select cdna subtraction technique

Environmental abiotic stresses, such as drought, high-salinity and low temperature, severely impair plant growth and development and limit crop productivity. In order to survive and adapt to these stresses, plants must induce various physiological, bichemical and molecular changes, including the adaptation of the photosynthetic apparatus, changing in the membrane lipid, the activation of calcium influxes and Ca2+-dependent protein kinase cascades, the accumulation of proline, glycine betaine, soluble sugars and increasing the levels of antioxidants. All these changes are accompanied by notable increases or decreases in the transcript level of specific genes. Hence, transcriptional control of stress-regulated genes is a crucial part of plant responses to abiotic stresses; a further characterization of such gene transcripts in plants may help us to understand the molecular basis of the plant response to abiotic stresses and to identify new targets for manipulating biochemical, physiological and developmental processes in plants. To clarify the process of the response of maize to cold stress and to discover maize genes associated with the response pathway(s), genes induced by cold treatment were isolated according to the PCR-select cDNA subtraction method. 18 cold-induced genes (ZmCOI) were detected at 6°C. They were divided into 6 groups, based on their functions. The cold induction of these genes was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) analyses. The sequences of these 18 cold-induced genes have been deposited in GenBank under accesion numbers from DQ078760 to DQ078778

Article 3: Characterization of the stress-induced gene ZmCOI6.1 in maize: Expression and promoter sequences

Using cDNA subtraction technique, 18 cold stress responsive-genes were identified, among them a novel gene, ZmCOI6.1, whose function is still unknown. Analysis of the ZmCOI6.1 promoter sequence revealed several conserved stress-responsive cis-acting elements. Further expression characterization shows that ZmCOI6.1 is induced, in addition by cold, by other  abiotic stresses such as drought and NaCl as well as by signalling molecules such as ABA and SA. The results indicate that ZmCOI6.1 is a general stress responsive gene. A possible regulation mechanism is presented where ZmCOI6.1 is alternatively spliced yielding two transcripts whose levels are changed upon different stress treatments. Furthermore the predicted ZmCOI6.1 amino acid sequence and its homologue show high similarity with proteins in rice and Arabidopsis suggesting that it belongs to a conserved protein in plant