Acceleration effects of microbial inoculum on palm oil mill organic waste composting.

ABSTRACT The acceleration effects of inoculum in composting of empty fruit bunches were investigated. Composting of empty fruit bunches fibres in two sizes, 4 cm and 2 cm length, were treated with microbial inoculum consisting of Agromonas, Aspergillus, Azotobacter, Bacillus, Celhdomonas, Chaetomium, Clostridium, Coprinus, Microbispora, Penicillium, Pseudomonas, Thermoactinomyces, Trichoderma and Trichurus in separate laboratory scale in-vessel of 30 liters volume. A control without inoculum with 4 cm length empty fruit bunches was also conducted in parallel. The compost piles were shift-turned weekly. Parameters such as moisture content, temperature, pH, and electrical conductivity were used to monitor the composting processes. The carbon-nitrogen ratio, UV-vis spectrophotometer test, and germination test were used to assess the maturity of compost. The results showed that the inoculum was effective in reducing the C/N ratio by 54% compared to control 46% and rapidly increasing the UV-vis absorption ratio in first three weeks. By using functional microbes, the composting of empty fruit bunches was reduced to 5 weeks compared to 9 weeks for those without inoculation. The acceleration effect was more prominent for the 2 cm length samples

Tailored watermarking schemes for authentication of electronic clinical atlases

10.1109/TITB.2005.855556IEEE Transactions on Information Technology in Biomedicine94554-563ITIB

Privacy and Ownership Preserving of Outsourced Medical Data

The demand for the secondary use of medical data is increasing steadily to allow for the provision of better quality health care. Two important issues pertaining to this sharing of data have to be addressed: one is the privacy protection for individuals referred to in the data; the other is copyright protection over the data. In this paper, we present a unified framework that seamlessly combines techniques of binning and digital watermarking to attain the dual goals of privacy and copyright protection. Our binning method is built upon an earlier approach of generalization and suppression by allowing a broader concept of generalization. To ensure data usefulness, we propose constraining Binning by usage metrics that define maximal allowable information loss, and the metrics can be enforced off-line. Our watermarking algorithm watermarks the binned data in a hierarchical manner by leveraging on the very nature of the data. The method is resilient to the generalization attack that is specific to the binned data, as well as other attacks intended to destroy the inserted mark. We prove that watermarking could not adversely interfere with binning, and implemented the framework. Experiments were conducted, and the results show the robustness of the proposed framework

DSIM: A Distance-Based Indexing Method for Genomic Sequences

10.1109/BIBE.2005.24Proceedings - BIBE 2005: 5th IEEE Symposium on Bioinformatics and Bioengineering200597-10

Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles

We describe a novel protocol for three-dimensional culturing of olfactory ensheathing cells (OECs), which can be used to understand how OECs interact with other cells in three dimensions. Transplantation of OECs is being trialled for repair of the paralysed spinal cord, with promising but variable results and thus the therapy needs improving. To date, studies of OEC behaviour in a multicellular environment have been hampered by the lack of suitable three-dimensional cell culture models. Here, we exploit the floating liquid marble, a liquid droplet coated with hydrophobic powder and placed on a liquid bath. The presence of the liquid bath increases the humidity and minimises the effect of evaporation. Floating liquid marbles allow the OECs to freely associate and interact to produce OEC spheroids with uniform shapes and sizes. In contrast, a sessile liquid marble on a solid surface suffers from evaporation and the cells aggregate with irregular shapes. We used floating liquid marbles to co-culture OECs with Schwann cells and astrocytes which formed natural structures without the confines of gels or bounding layers. This protocol can be used to determine how OECs and other cell types associate and interact while forming complex cell structuresJSJ was funded by a grant from the Perry Cross Spinal Research Foundation; NTN was funded from Griffith University through a start-up grant and a grant from the Griffith University Research Infrastructure Program; JAK was funded by an Australian Research Council Discovery Grant DP150104495; JT was funded by an Eskitis Institute scholarship; CO was funded by a Griffith Sciences scholarship; RV was funded by a Griffith University International Postgraduate Research Scholarshi