Mechanistic understanding of iron toxicity tolerance in contrasting rice varieties from Africa: 1. Morpho-physiological and biochemical responses

Iron (Fe) is a fundamental element involved in various plant metabolic processes. However, when Fe uptake is excessive, it becomes toxic to the plant and disrupts cellular homeostasis. The aim of this study was to determine the physiological and biochemical mechanisms underlying tolerance to Fe toxicity in contrasting rice varieties adapted to African environments. Four varieties (CK801 and Suakoko 8 (tolerant), Supa and IR64 (sensitive)) selected from our previous work were analysed in more detail, and the first part of this study reports morphological, physiological and biochemical responses induced by Fe toxicity in these four varieties. Morphological (shoot length, root length, number of lateral roots), physiological (photosynthesis rate, stomatal conductance, transpiration rate, fluorescence, relative water content and cell membrane stability) and biochemical (tissue Fe, chlorophyll pigments, soluble sugars, protein and starch) traits were measured, as appropriate, on both shoot and root tissues and at different time points during the stress period. Fe toxicity significantly (P ≤ 0.05) reduced growth and metabolism of all the four varieties. Tolerant varieties showed more lateral roots than the sensitive ones, under Fe toxic conditions as well as higher photosynthesis rate, chlorophyll content and cell membrane stability. Strong dilution of Fe concentration in cells was identified, as one of the additional tolerance mechanisms used by CK801, whereas Suakoko 8 mainly used strong mobilisation of carbohydrates at the early stage of the stress period to anticipate metabolite shortage. Traits associated with Fe toxicity tolerance in this study could be specifically targeted in trait-based breeding programs of superior lowland rice varieties tolerant of Fe toxicity

Global Financial Education: A Strategy to Advance Microfinance

The research was financed by ESADE Business School (Sponsoring the researcher in his PhD studies)'AbstractThere is growing evidence that microfinance institutions (MFIs) have substantial benefits for consumers, especially women in financial inclusion. Several attempts have been made to explain why MFIs have not been able to bridge to financial inclusion. Prior research has addressed the reasons why globally; two-thirds of adults do not have an account. However, little is known about the role of financial education that leads to financial literacy especially regarding the method, mode, form and subject. This study proposes Global Financial Education (GFE)that would lead to financial literacy among MFI clients in order to empower them to select appropriate MFIs, MFI products and services, right channels for greater financial inclusion. We propose a practical mode of implementing GFE. This is likely to propel financial inclusion as opposed to merely access to credit through informal financial institutions where clients are caught in a vicious cycle of poverty, unable to transition to middle class status.Keywords: Financial Inclusion; Financial Literacy; Global Financial Education; Micro-Financial Institutions;DOI: 10.7176/JPID/55-05Publication date:August 31st 202

Functionalised natural zeolite and its potential for treating drinking water containing excess amount of nitrate

An excess level of nitrate in drinking water is responsible for methemoglobinemia, or ‘blue baby’ disease. Consequently, management of nitrate in drinking water is universally of public health interest. In this study, clinoptilolite functionalised with cationic surfactant hexadecyltrimethyl ammonium (HDTMA) was used for the removal of nitrate from drinking water. The effects of surfactant loading, adsorbent dosage, pH, coexisting ions, temperature and environmental water quality on the adsorption of nitrate were investigated. It was found that adsorption was optimum when the initial concentration of the functionalising surfactant was 3 000 mg∙ℓ-1. An increase in adsorbent dosage raised the percentage removal of nitrate. The valency of the coexisting anion had a major effect on nitrate removal, with the presence of phosphate (a trivalent anion) leading to the lowest removal ability. Temperature and pH had negligible effects on adsorbent performance. Environmental water samples (natural groundwater samples from Limpopo Province, South Africa) were tested and it was found that the World Health Organisation (WHO) regulatory compliance can be achieved even when the initial concentration of nitrate exceeds 300 mg∙ℓ-1. Equilibrium data was modelled using the Freundlich and Langmuir isotherms and the data conformed well to the Freundlich isotherm, indicating the heterogeneous nature of the active sites. Kinetically, nitrate adsorption was best described by the pseudo-second rate equation.Keywords: adsorption, zeolite, nitrate, kinetics, equilibriu

Antibiotic Sensitivity Patterns of Aerobic Bacterial Agents in Post-Surgical Orofacial Infections

Background: There is no data on the bacteriology of postsurgical orofacial infections at our institutions. Uncontrolled use of antibiotics may lead to development of resistance with the resultant increase in morbidity and cost of treatment. We aimed to determine the aerobic bacterial agents and antibiotic sensitivity of post-surgical infections in the orofacial region. Methodology: Patients were evaluated for post-surgical wound infection from the 5th to 30th post-operative day. In cases where a surgical implantwas placed the period of review was extended for up twelve months. The specimens were collected using sterile swabs and transported to the  microbiology laboratory within two hours of collection. The specimens were then analyzed for bacteriology according to the standard bacteriological  techniques. A wide range of antibiotics including those commonly used to treat orofacial infections were tested for sensitivity against the isolates obtained using the disk diffusion test (Kirby-Bauer procedure, using CLSI protocols). Results: Staphylococcus aureus accounted for 40% of the isolates followedby Klebsiella species (23%) and the Pseudomonas species (19%).  Amoxycillin/clavulinic acid, the 2nd and 3rd generation cephalosporins were effective against most of the bacterial infections from the orofacial region. Conclusion: Staphylococcus aureus, Klebsiella, and Pseudomonas species arethe commonest isolates from the oral facial region. Antibiotics which showed adequate efficacy against them were the augmented Penicillins and newer generations Cephalosporins.Key Words: Oro-facial infection, Post-surgical, Antibiotic

What Extension Personnel Should Know About Midwestern Goat Producers

The growth of the goat industry has created opportunities for producers looking for a profitable alternative enterprise. Little is known about goat production or educational needs of producers in Missouri and Arkansas. A survey of goat producers in Missouri and Arkansas addressed farm characteristics, product marketing characteristics, preferred information sources, and educational topics of interest for goat producers. A better understanding of goat production and producer needs can support Extension\u27s development of education programs to further develop the emerging goat industry

Determining efficient extraction procedure of phytochemicals from the fruit paste of Ziziphus abyssinica and Tamarindus indica.

Objective: To determine the most effective method of extracting metabolites from the two herbs Ziziphus abyssinica and Tamaridus. Indicus.Methodology and results: The methods used included cold and soxhlet extraction using methanol as the solvent and hot extraction using distilled water. To determine the efficiency in which compounds are extracted TLC was performed on silica gel aluminium plates using ethyl acetate: formic acid: glacial acetic acid: water (100:11:11:27). To determine the quantity of phenolic compounds in the extract, the Folin and Ciocalteau’s method (1927) was used, using Gallic acid in various concentrations. For the total quantity of flavonoid compounds, the method of Miliauskas et al. (2004) was used. To determine the Proanthocyanidin content in the extract a method previously reported by Ayoola et al, 2006 was used. To measure the antioxidant capacity of the extracts the hydrogen donating or free radical scavenging activity, was measured using the stable radical DPPH. The compounds extracted by all the methods were about seven but the difference was noted when the individual compounds were analysed. The cold extraction on the herb extract of Z. abyssinica had significantly high amount of total phenols 1.99g/100g of sample than both soxhlet and water extraction with 1.51g and 0.61g/100g of sample respectively. The results of T. indicus indicate that the extracts from the soxhlet and cold extraction methods contained a significantly low amount of all the three compounds compared to the water extractsConclusion and application of results: The method best suited for obtaining extracts from the two herbs T. indicus and Z. abyssinica is, cold method of extraction with methanol as the solvent for Z. abyssinica and hot extraction using distilled water for T. indicus. The results obtained give guidance to the fact that using both herbs would result in a better preservative than using one herb since the identified compounds would complement each other.Key words: Antimicrobial, antioxidant, phytochemicals and Radical scavenging activity

Molecular Markers for Genetic Diversity Studies in African Leafy Vegetables

African leafy vegetables are becoming important crops in tackling nutrition and food security in many parts of sub-Saharan Africa, since they provide important micronutrients and vitamins, and help resource-poor farm families bridge lean periods of food shortage. Genetic diversity studies are essential for crop improvement programmes as well as germplasm conservation efforts, and research on genetic diversity of these vegetables using molecular markers has been increasing over time. Diversity studies have evolved from the use of morphological and biochemical markers to molecular markers. Molecular markers provide valuable data, since they detect mostly selectively neutral variations at the DNA level. They are well established and their strengths and limitations have been described. New marker types are being developed from a combination of the strengths of the basic techniques to improve sensitivity, reproducibility, polymorphic information content, speed and cost. This review discusses the principles of some of the established molecular markers and their application to genetic diversity studies of African leafy vegetables with a main focus on the most common Solanum, Amaranthus, Cleome and Vigna species.BMBF/HORTINLEABM

Community opinion leaders in Ghana speak out on adolescent sexuality: What are the issues?

This policy brief reports on a study that is a component of a larger study that assessed the sexual and reproductive health (SRH) needs of adolescents in four slums in two regions of Ghana. The study’s focus on this perspective stems from the fact that there is a lack of evidence surrounding the outlook of community opinion leaders on adolescent reproductive health in this setting. Understanding what adults think about major adolescent SRH issues in their communities (including the SRH rights of adolescents, adult–adolescent communication about SRH issues, and service-seeking and sexual behavior outcomes) provides insight into how adults perceive and influence the lives of young people, and therefore, how programs could be designed to better serve the sexual health needs of adolescents. The brief summarizes key findings that identify the issues facing these community opinion leaders

Predictability of the Arctic sea ice edge

Skillful sea ice forecasts from days to years ahead are becoming increasingly important for the operation and planning of human activities in the Arctic. Here we analyze the potential predictability of the Arctic sea ice edge in six climate models. We introduce the integrated ice-edge error (IIEE), a user-relevant verification metric defined as the area where the forecast and the “truth” disagree on the ice concentration being above or below 15%. The IIEE lends itself to decomposition into an absolute extent error, corresponding to the common sea ice extent error, and a misplacement error. We find that the often-neglected misplacement error makes up more than half of the climatological IIEE. In idealized forecast ensembles initialized on 1 July, the IIEE grows faster than the absolute extent error. This means that the Arctic sea ice edge is less predictable than sea ice extent, particularly in September, with implications for the potential skill of end-user relevant forecasts