Moving Arts Leadership Forward: A Changing Landscape

Since 2009, the William and Flora Hewlett Foundation's Performing Arts Program has been making grants to help emerging arts leaders develop satisfying and successful careers through the Next Generation Arts Leadership Initiative. The first phase of that work, which ended in 2015, was funded in partnership with the James Irvine Foundation. It focused on training and retaining emerging arts leaders -- defined as eighteen to thirty-five-year-olds with ten years or less of arts experience -- in anticipation of a widely predicted wave of retirements. The Initiative made grants totaling $1.9 million to five leadership networks across California, and to statewide regranting programs, managed by the Center for Cultural Innovation to support professional development for individuals and innovative organizational practices. While an assessment conducted in 2011 showed that the Initiative was successful in achieving its early goals of building infrastructure and opportunities for younger arts leaders, the Performing Arts Program and our partners continued to grapple with a few persistent questions: what were we preparing up-and-coming leaders to do? To what degree did we aim to sustain the field as it exists or spur its transformation? Were we adequately preparing leaders for the challenges to come? To help answer these questions, in late 2014 we commissioned Michael Courville of Open Mind Consulting to reassess the arts leadership landscape in California and explore opportunities for future investments in arts leadership.The research was conducted in collaboration with a cross-section of local, regional, and national arts leaders, and with the Initiative's partners. It reveals that the arts landscape is in a state of flux and that there is a timely opportunity to reimagine how the nonprofit arts field defines and practices leadership

La construction de l’identité dans une société de la diversité. Critique du multiculturalisme de Kymlicka et de Taylor

Les sociétés modernes sont de plus en plus souvent confrontées aux enjeux de la diversité. Le multiculturalisme tente d’y apporter une réponse à travers un modèle de société basé sur une politique de la reconnaissance. Selon moi, l’argumentation des multiculturalistes repose sur leur conception de l’identité. C’est pourquoi, pour apporter une critique nouvelle de ce courant de pensée, j’analyse les écrits de ses principaux défenseurs, Will Kymlicka et Charles Taylor, en concentrant mon analyse sur ce qu’ils entendent par identité. Je soutiens que leur conception ne laisse pas assez de place au potentiel d’évolution et ne considère pas suffisamment l’importance et le pouvoir de la volonté individuelle dans la construction de l’identité. En m’appuyant sur l’étude d’autres auteurs, je souligne la nécessité de considérer les frontières entre le semblable et l’autre de manière plus souple et de reconnaître que la violence peut s’exercer par la création de différences entre les personnes. J’ai choisi la figure radicale de « l’homme sans qualités » de Robert Musil pour montrer comment un individu peut devenir autonome à travers un acte de destruction créatrice. Enfin, je conclus que l’État devrait favoriser ce processus, et permettre au sujet, qui est selon moi dénué d’une nature authentique, de penser son existence comme une expérience du possible.Modern societies are increasingly confronted with the challenge of diversity. Defenders of multiculturalism responded with a model of society based on a politics of recognition. This model can be connected to a particular conception of identity. In order to make a new contribution to the critique of this approach, this work presents an analysis of the writings of two of the main proponents: Will Kymlicka and Charles Taylor. It does so by focusing on their conception of identity and argues that it does not leave enough room for change and fails to fully recognise the importance and the impact of personal will. The works of other authors are analysed in order to emphasize the need to view the boundaries between sameness and otherness in a more flexible fashion and to acknowledge that violence can attend the creation of differences between people. The figure of the “man without qualifies” portrayed by the novelist Robert Musil is offered as emblematic of how an individual can become autonomous through an act of creative destruction. Finally, it is concluded that the state should facilitate the creation of difference and this requires allowing citizens to conceive of their lives as an experience of the possible

Pseudomonas Aeruginosa Sabotages the Generation of Host Proresolving Lipid Mediators

Recurrent Pseudomonas aeruginosa infections coupled with robust, damaging neutrophilic inflammation characterize the chronic lung disease cystic fibrosis (CF). The proresolving lipid mediator, 15-epi lipoxin A4 (15-epi LXA4), plays a critical role in limiting neutrophil activation and tissue inflammation, thus promoting the return to tissue homeostasis. Here, we show that a secreted P. aeruginosa epoxide hydrolase, cystic fibrosis transmembrane conductance regulator inhibitory factor (Cif), can disrupt 15-epi LXA4 transcellular biosynthesis and function. In the airway, 15-epi LXA4 production is stimulated by the epithelial-derived eicosanoid 14,15-epoxyeicosatrienoic acid (14,15-EET). Cif sabotages the production of 15-epi LXA4 by rapidly hydrolyzing 14,15-EET into its cognate diol, eliminating a proresolving signal that potently suppresses IL-8-driven neutrophil transepithelial migration in vitro. Retrospective analyses of samples from patients with CF supported the translational relevance of these preclinical findings. Elevated levels of Cif in bronchoalveolar lavage fluid were correlated with lower levels of 15-epi LXA4, increased IL-8 concentrations, and impaired lung function. Together, these findings provide structural, biochemical, and immunological evidence that the bacterial epoxide hydrolase Cif disrupts resolution pathways during bacterial lung infections. The data also suggest that Cif contributes to sustained pulmonary inflammation and associated loss of lung function in patients with CF

N,N-Dimethylaminoxy Carbonyl, a Polar Protecting Group for Efficient Peptide Synthesis

Peptide coupling with minimal protection is one of the desired methods for the synthesis of peptides and proteins. To achieve regioselective amide bond formation, side chain protection is often essential; however, protecting groups potentially diminish peptide solubility and render the polar polyamide chain amphipathic due to their apolar nature. In this manuscript, we describe a new protecting group, N,N-dimethylaminoxy carbonyl (Dmaoc), and its use in peptide coupling reactions. The Dmaoc group has a relatively polar character compared to the Boc group, which is a conventional protecting group for the Nε-amine of Lys residues. This polar protecting group is removable by reduction in the buffer containing (±)-dithiothreitol (DTT). Furthermore, the Dmaoc group proved compatible with peptide ligation strategies featuring the activation of N-acyl diaminobenzamides (Dbz) with sodium nitrate to generate the respective benzotriazole leaving group. The Dmaoc/Dbz strategy described in this manuscript provides a new method for the chemical synthesis of peptides

Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps

We previously reported that chronic rhinosinusitis with nasal polyps (CRSwNP) was subdivided into four chronic rhinosinusitis (CRS) subtypes using the JESREC scoring system. We sought to identify the gene expression profile and biomarkers related with CRSwNP by RNA-sequence. RNA-sequencing was performed to identify differentially expressed genes between nasal polyps (NPs) and inferior turbinate mucosa from 6 patients with CRSwNP, and subsequently, quantitative real-time PCR was performed to verify the results. ELISA was performed to identify possible biomarkers for postoperative recurrence. In the RNA-sequencing results, periostin (POSTN) expression was the highest in NP. We focused on POSTN and investigated the protein level of POSTN by immunohistochemistry and ELISA. POSTN was diffusely expressed in moderate and severe eosinophilic CRS using immunohistochemistry, and its staining pattern was associated with the severity of the phenotype of the CRSwNP (P < 0.05). There was a significant difference between the POSTN high/low groups for postoperative recurrence when the cutoff point was set at 115.5 ng/ml (P = 0.0072). Our data suggests that the protein expression level of POSTN was associated with the severity of CRSwNP, and serum POSTN can be a novel biomarker for postoperative recurrence of CRSwNP

Substitution of the myristoylation signal of human immunodeficiency virus type 1 Pr55Gag with the phospholipase C-δ1 pleckstrin homology domain results in infectious pseudovirion production

The matrix domain (MA) of human immunodeficiency virus type 1 Pr55Gag is covalently modified with a myristoyl group that mediates efficient viral production. However, the role of myristoylation, particularly in the viral entry process, remains uninvestigated. This study replaced the myristoylation signal of MA with a well-studied phosphatidylinositol 4,5-biphosphate-binding plasma membrane (PM) targeting motif, the phospholipase C-δ1 pleckstrin homology (PH) domain. PH–Gag–Pol PM targeting and viral production efficiencies were improved compared with Gag–Pol, consistent with the estimated increases in Gag–PM affinity. Both virions were recovered in similar sucrose density-gradient fractions and had similar mature virion morphologies. Importantly, PH–Gag–Pol and Gag–Pol pseudovirions had almost identical infectivity, suggesting a dispensable role for myristoylation in the virus life cycle. PH–Gag–Pol might be useful in separating the myristoylation-dependent processes from the myristoylation-independent processes. This the first report demonstrating infectious pseudovirion production without myristoylated Pr55Gag

乳幼児を育てる母親における親族以外の子育て支援者の実態と支援内容の特徴

本研究では、地域社会全体による子育て支援に向けて、乳幼児を育てる母親における親族以外の日常的な子育てに関する支援者の実態を把握するとともに、親族以外の支援者による支援内容の特徴について検討することを目的とした。乳幼児を育てる母親を対象に無記名の質問紙調査を実施し、218人から得た回答を分析対象とした。親族以外の支援者では、主に近くに住む同年代の知人・友人が最も重要な支援者となっていることがわかった。また日常的な子育てに関する支援は親族内から得ていることが多いが、内容によっては親族内よりも親族以外から支援を得ていることが推測された。親族以外の支援者による支援内容の特徴については、情緒的な支援や情報的な支援は比較的提供されているものの、直接的な援助となる手段的な支援は提供されにくいことが示唆された