Sexual risky behaviour among Slovak adolescents and young adults:social and psychological factors

Het centrale doel van dit proefschrift was om de relatie tussen seksueel risicovol gedrag (SRB) van adolescenten en jong volwassenen en gedragsfactoren verkennen (bijvoorbeeld het gebruik van alcohol, vorige seksueel gedrag), psychologische factoren (bv. extraversie, welzijn) en sociale factoren (bijvoorbeeld familie structuur, opvoeding, sociale steun). Behavioural factoren lijken te zijn nauw verwant aan SRB dan (andere) psychische factoren. Factoren zoals dronken en met vroege seks waren sterk geassocieerd met SRB (meerdere seksuele partners en seks in risicovolle omstandigheden) van de jonge volwassen mannen en vrouwen. Van de vijf verkend psychologische factoren slechts twee (extraversie en religiositeit) waren geassocieerd met SRB. Bovendien studenten die gemeld seks te hebben gehad voor de eerste keer op de leeftijd van 17 of hoger waren significant minder kans om te rapporteren die deelnemen aan de twee soorten SRB wordt onderzocht (sex onder alcohol-of drugsgebruik invloed is geweest en meerdere seksuele partners). Bij adolescenten ons onderzoek over de ouderlijke processen bleek dat ouderlijk toezicht is sterker geassocieerd met SRB dan ouderlijke sociale steun. In het bijzonder werd een lagere controle door de vader significant geassocieerd met de vroege eerste geslachtsgemeenschap bij meisjes en met inconsistente gebruik van condooms bij jongens. Aan de andere kant psychosociale variabelen, zoals angst, sociale steun van anderen en vrienden, sociaal disfunctioneren en positief gevoel van eigenwaarde onderscheid maken tussen jongeren die seks hebben gehad (zowel riskant of veilig) en adolescenten die dat niet hadden, maar ze maken geen onderscheid tussen adolescenten die last hebben gehad risicovolle seks en degenen die hebben gehad veilig vrijen

Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood

Background: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory B-cell (MBC) and plasma cell (PCs) compartments remains to be investigated. Objective: We studied the age-related distribution of MBCs and PCs expressing different IgH isotypes in addition to the immature/transitional and naive B-cell compartments. Methods: B-cell and PC subsets and plasma IgH isotype levels were studied in cord blood (n = 19) and peripheral blood (n = 215) from healthy donors aged 0 to 90 years by using flow cytometry and nephelometry, respectively. Results: IgH-switched MBCs expressing IgG1, IgG2, IgG3, IgA1, and IgA2 were already detected in cord blood and newborns at very low counts, whereas CD27+IgM++IgD+ MBCs only became detectable at 1 to 5 months and remained stable until 2 to 4 years, and IgD MBCs peaked at 2 to 4 years, with both populations decreasing thereafter. MBCs expressing IgH isotypes of the second immunoglobulin heavy chain constant region (IGHC) gene block (IgG1, IgG3, and IgA1) peaked later during childhood (2-4 years), whereas MBCs expressing third IGHC gene block immunoglobulin isotypes (IgG2, IgG4, and IgA2) reached maximum values during adulthood. PCs were already detected in newborns, increasing in number until 6 to 11 months for IgM, IgG1, IgG2, IgG3, IgA1, and IgA2; until 2 to 4 years for IgD; and until 5 to 9 years for IgG4 and decreasing thereafter. For most IgH isotypes (except IgD and IgG4), maximum plasma levels were reached after PC and MBC counts peaked. Conclusions: PC counts reach maximum values early in life, followed by MBC counts and plasma IgH isotypes. Importantly, IgH isotypes from different IGHC gene blocks show different patterns, probably reflecting consecutive cycles of IgH isotype switch recombination through life

Personal and perceived peer use and attitudes towards use of non-prescribed prescription sedatives and sleeping pills among university students in seven European countries

Introduction The use of non-prescribed prescription sedatives and sleeping pills (NPPSSP) among university students has been described as an important public health issue. However, the impact of perceived social norms on students' use and attitudes towards use of NPPSSP is still unclear. Our aim was to investigate whether perceptions of peer use and approval of use are associated with students' personal use and approval of NPPSSP use. Methods Cross-sectional data from the Social Norms Intervention for the prevention of Polydrug Use (SNIPE) project containing 4482 university students from seven European countries were analyzed to investigate self-other discrepancies regarding personal use and attitudes towards NPPSSP use. Associations between personal and perceived peer use and between personal and perceived approval of use were examined using multivariable logistic regression. Results The majority (51.0%) of students perceived their peers' NPPSSP use to be higher than their personal use. 92.6% of students perceived their peers' approval of NPPSSP use to be identical or higher than their personal approval. Students perceiving that the majority of peers had used NPPSSP at least once displayed higher odds for personal lifetime use (OR: 1.95, 95% CI: 1.49–2.55). Perceived peer approval of NPPSSP use was associated with higher odds for personal approval (OR: 5.49, 95% CI: 4.63–6.51). Conclusions Among European university students, perceiving NPPSSP use and approval of use to be the norm was positively associated with students' personal NPPSSP use and approval of use, respectively. Interventions addressing perceived social norms may prevent or reduce NPPSSP use among university students

Myeloid antigens in childhood lymphoblastic leukemia:clinical data point to regulation of CD66c distinct from other myeloid antigens

BACKGROUND: Aberrant expression of myeloid antigens (MyAgs) on acute lymphoblastic leukemia (ALL) cells is a well-documented phenomenon, although its regulating mechanisms are unclear. MyAgs in ALL are interpreted e.g. as hallmarks of early differentiation stage and/or lineage indecisiveness. Granulocytic marker CD66c – Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is aberrantly expressed on ALL with strong correlation to genotype (negative in TEL/AML1 and MLL/AF4, positive in BCR/ABL and hyperdiploid cases). METHODS: In a cohort of 365 consecutively diagnosed Czech B-precursor ALL patients, we analyze distribution of MyAg+ cases and mutual relationship among CD13, CD15, CD33, CD65 and CD66c. The most frequent MyAg (CD66c) is studied further regarding its stability from diagnosis to relapse, prognostic significance and regulation of surface expression. For the latter, flow cytometry, Western blot and quantitative RT-PCR on sorted cells is used. RESULTS: We show CD66c is expressed in 43% patients, which is more frequent than other MyAgs studied. In addition, CD66c expression negatively correlates with CD13 (p < 0.0001), CD33 (p = 0.002) and/or CD65 (p = 0.029). Our data show that different myeloid antigens often differ in biological importance, which may be obscured by combining them into "MyAg positive ALL". We show that unlike other MyAgs, CD66c expression is not shifted from the onset of ALL to relapse (n = 39, time to relapse 0.3–5.3 years). Although opposite has previously been suggested, we show that CEACAM6 transcription is invariably followed by surface expression (by quantitative RT-PCR on sorted cells) and that malignant cells containing CD66c in cytoplasm without surface expression are not found by flow cytometry nor by Western blot in vivo. We report no prognostic significance of CD66c, globally or separately in genotype subsets of B-precursor ALL, nor an association with known risk factors (n = 254). CONCLUSION: In contrast to general notion we show that different MyAgs in lymphoblastic leukemia represent different biological circumstances. We chose the most frequent and tightly genotype-associated MyAg CD66c to show its stabile expression in patients from diagnosis to relapse, which differs from what is known on the other MyAgs. Surface expression of CD66c is regulated at the gene transcription level, in contrast to previous reports

European Language Grid: A Joint Platform for the European Language Technology Community

Europe is a multilingual society, in which dozens of languages are spoken. The only option to enable and to benefit from multilingualism is through Language Technologies (LT), i.e., Natural Language Processing and Speech Technologies. We describe the European Language Grid (ELG), which is targeted to evolve into the primary platform and marketplace for LT in Europe by providing one umbrella platform for the European LT landscape, including research and industry, enabling all stakeholders to upload, share and distribute their services, products and resources. At the end of our EU project, which will establish a legal entity in 2022, the ELG will provide access to approx. 1300 services for all European languages as well as thousands of data sets