Clinicopathological Studies on the Effect of Nano Selenium Particles in Broilers

The reason for this research was to analyze the potential immune-stimulating and antioxidant properties of nano-selenium (nano-Se) in broiler chickens. The study utilized 150 one-day-old Cobb broiler chickens, which were arbitrarily allocated to six groups of 25 chickens every: G1 (control), G2 (0.3 ml nano-selenium/L water), G3 (0.5 ml nano-selenium/L water), G4 (E. coli 2 x 107 cfu), G5 (0.3 ml nano-selenium/L water and E. coli), and G6 (0.5 ml nano-selenium/L water and E. coli). Various immune response, antioxidant, and oxidative stress parameters were evaluated. The results revealed that infected chickens had significantly lower levels of immunoglobulins (IgG, IgM, IgA), glutathione peroxidase (GPX), superoxide dismutase (SOD), and interleukin-4 (IL-4) compared to the control group. Conversely, the infected chickens revealed a marked higher in interleukin-2 (IL-2), interferon-gamma (IF-γ), and malondialdehyde (MDA). In contrast, infected and nano-se treated chickens exhibited a rise in IgG, IgM, IgA, GSH, GPX, SOD, and IL-4 with a notable decline in IF-γ, IL-2, and MDA relative to the infected group. These findings suggest that nano-se may play a significant role in immune response, antioxidant activity, and control and prevention of E. coli infections in broiler chickens. These results imply that nano-se may have a substantial role in strengthening the immune response, antioxidant activity, and management and prevention of E. coli infections in broiler chickens.Bottom of Form &nbsp

Black Seed Thymoquinone Improved Insulin Secretion, Hepatic Glycogen Storage, and Oxidative Stress in Streptozotocin-Induced Diabetic Male Wistar Rats

Diabetes mellitus is one of the metabolic diseases having several complications. Nigella sativa oil (NSO) might have beneficial effects in the treatment of diabetic complications. Thirty-two mature male Wistar rats were equally divided into four experimental groups: control, control NSO 2 mL/kg, streptozotocin- (STZ-) induced diabetic, and diabetic (STZ-induced) treated with oral NSO 2 mg/kg for 30 days. Fasting blood glucose (FBG), insulin, and lipid profile levels were determined. Pancreatic and hepatic tissues were used for catalase and GSH. Histopathology, hepatic glycogen contents, insulin immunohistochemistry, and pancreatic islet morphometry were performed. NSO 2 mL/kg was noticed to decrease (P<0.05) FBG and increase (P<0.05) insulin levels in diabetic rats than in diabetic nontreated animals. Lipid profile showed significant (P<0.5) improvement in diabetic rats that received NSO 2 mL/kg than in the diabetic group. Both pancreatic and hepatic catalase and GSH activities revealed a significant (P<0.05) increment in the diabetic group treated with NSO than in the diabetic animals. NSO improved the histopathological picture and hepatic glycogen contents of the diabetic group as well as increased (P<0.05) insulin immunoreactive parts % and mean pancreatic islet diameter. NSO exerts ameliorative and therapeutic effects on the STZ-induced diabetic male Wistar rats