SITE REMEDIATION IN NIGERIA: PROVEN AND INNOVATIVE TECHNOLOGIES (RECOVERY OF FREE HYDROCARBON FROM SOIL/GROUNDWATER)

This research is concerned with geo-environmental impact assessment and remediation of contaminated soil/groundwater. The specific study site is Baruwa community (Latitude 06˚ 35' 12'' N, Longitude 03˚ 16' 21'' E), in Alimosho Local government area of Lagos state, Nigeria. The estimated 65,000 people of Baruwa own about 350 hand dug wells for domestic water supply; more than 200 of these wells are at present, under lock and key because of the oil seepage from leaking underground NNPC (Nigerian National Petroleum Corporation) pipeline. The Geoenvironmental Engineering research group in F.U.T.Akure, started Geoenvironmental site assessment, geotechnical, background and natural attenuation studies for the site in 2006. This has enabled delineation of the contaminated zone. Eight (8) hydrocarbon recovery/monitoring wells (W16, W17, W18, W19, W20, W42, W43, and W44) and two bore holes (W401 and W402) were used for the pilot recovery test. Existing contaminated water supply wells were used as hydrocarbon recovery/monitoring wells to minimize cost. Two boreholes were installed for soil vapour extracting and gas surveys in addition to the eight existing wells within the 100m x 100m designated pilot test area. We have already established that water supply wells are covered by pure phase leaking hydrocarbon products from the underground pipes. Tested well waters had been found to be as much as 95 percent petrol and up to 600 mm thickness of free hydrocarbon product on top of the ground water. Volumetric characterization of the pure phase hydrocarbon using Oil/Water Interface meter/ areal measurements and monitoring together with assessment of various technologies (Natural Attenuation, In Situ Chemical Oxidation and Bioremediation) for remediation was implemented for this site

Contamination potential of tar sand exploitation in the western Niger-Delta of Nigeria: baseline studies

Exploitation of the tar sands in Ondo State in the Niger Delta is likely to result in some environmental contamination and adversely affect the living conditions of the farmers and fishermen in the area. Three wells were established where samples were taken at 1, 2 and 3 m depth. Although no hydrocarbon contamination was detected in the water from the surface and boreholes, saline intrusion was recorded. This study produces a baseline against which future contamination can be monitored so that preventative measures can be taken to mitigate the effects

Dynamics in the murine norovirus capsid revealed by high-resolution cryo-EM

Icosahedral viral capsids must undergo conformational rearrangements to coordinate essential processes during the viral life cycle. Capturing such conformational flexibility has been technically challenging yet could be key for developing rational therapeutic agents to combat infections. Noroviruses are nonenveloped, icosahedral viruses of global importance to human health. They are a common cause of acute gastroenteritis, yet no vaccines or specific antiviral agents are available. Here, we use genetics and cryo-electron microscopy (cryo-EM) to study the high-resolution solution structures of murine norovirus as a model for human viruses. By comparing our 3 structures (at 2.9- to 3.1-Å resolution), we show that whilst there is little change to the shell domain of the capsid, the radiating protruding domains are flexible, adopting distinct states both independently and synchronously. In doing so, the capsids sample a range of conformational space, with implications for maintaining virion stability and infectivity

A year of genomic surveillance reveals how the SARS-CoV-2 pandemic unfolded in Africa

[Figure: see text]

The evolving SARS-CoV-2 epidemic in Africa: insights from rapidly expanding genomic surveillance

Investment in SARS-CoV-2 sequencing in Africa over the past year has led to a major increase in the number of sequences generated, now exceeding 100,000 genomes, used to track the pandemic on the continent. Our results show an increase in the number of African countries able to sequence domestically, and highlight that local sequencing enables faster turnaround time and more regular routine surveillance. Despite limitations of low testing proportions, findings from this genomic surveillance study underscore the heterogeneous nature of the pandemic and shed light on the distinct dispersal dynamics of Variants of Concern, particularly Alpha, Beta, Delta, and Omicron, on the continent. Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve, while the continent faces many emerging and re-emerging infectious disease threats. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Emergence and spread of two SARS-CoV-2 variants of interest in Nigeria.

Identifying the dissemination patterns and impacts of a virus of economic or health importance during a pandemic is crucial, as it informs the public on policies for containment in order to reduce the spread of the virus. In this study, we integrated genomic and travel data to investigate the emergence and spread of the SARS-CoV-2 B.1.1.318 and B.1.525 (Eta) variants of interest in Nigeria and the wider Africa region. By integrating travel data and phylogeographic reconstructions, we find that these two variants that arose during the second wave in Nigeria emerged from within Africa, with the B.1.525 from Nigeria, and then spread to other parts of the world. Data from this study show how regional connectivity of Nigeria drove the spread of these variants of interest to surrounding countries and those connected by air-traffic. Our findings demonstrate the power of genomic analysis when combined with mobility and epidemiological data to identify the drivers of transmission, as bidirectional transmission within and between African nations are grossly underestimated as seen in our import risk index estimates

A year of genomic surveillance reveals how the SARS-CoV-2 pandemic unfolded in Africa.

The progression of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in Africa has so far been heterogeneous, and the full impact is not yet well understood. In this study, we describe the genomic epidemiology using a dataset of 8746 genomes from 33 African countries and two overseas territories. We show that the epidemics in most countries were initiated by importations predominantly from Europe, which diminished after the early introduction of international travel restrictions. As the pandemic progressed, ongoing transmission in many countries and increasing mobility led to the emergence and spread within the continent of many variants of concern and interest, such as B.1.351, B.1.525, A.23.1, and C.1.1. Although distorted by low sampling numbers and blind spots, the findings highlight that Africa must not be left behind in the global pandemic response, otherwise it could become a source for new variants

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance.

Investment in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing in Africa over the past year has led to a major increase in the number of sequences that have been generated and used to track the pandemic on the continent, a number that now exceeds 100,000 genomes. Our results show an increase in the number of African countries that are able to sequence domestically and highlight that local sequencing enables faster turnaround times and more-regular routine surveillance. Despite limitations of low testing proportions, findings from this genomic surveillance study underscore the heterogeneous nature of the pandemic and illuminate the distinct dispersal dynamics of variants of concern-particularly Alpha, Beta, Delta, and Omicron-on the continent. Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve while the continent faces many emerging and reemerging infectious disease threats. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Engineering properties of tropical clay and bentonite modified with sawdust

Construction engineers typically avoid the use of expansive soils as construction materials because they are usually difficult to work on and can cause structural failure. This research work investigates how the application of sawdust to tropical clay and bentonite influences their geotechnical properties in order to determine their suitability for use as landfill-liner materials for the effective containment of toxic substances from landfills. X-ray diffractometry, X-ray fluorescence spectroscopy and scanning electron microscopy were used to determine the mineralogical composition, oxide composition and microstructure, respectively, of the clay and the bentonite. A series of laboratory tests were conducted to determine the specific gravity, Atterberg limits, compaction, unconfined compressive strength and permeability characteristics of the clay and the bentonite for varying proportions of sawdust application. Generally, increasing the percentage of sawdust caused a reduction in its specific gravity, maximum dry unit weight and unconfined compressive strength, while it caused an increase in the optimum moisture content and permeability of the modified clay and bentonite. The clay and bentonite both have a sufficiently low permeability that satisfies the hydraulic conductivity requirement for use as clay liners. Eight percent sawdust application to a clay having similar properties as that in this study is recommended as an economic way of modifying it – with the potential of improving its adsorbent property – for use in landfill-liner systems in order to prevent the toxic substances leaching from the landfills, thereby protecting the environment and public health