A mathematical model for the capillary endothelial cell-extracellular matrix interactions in wound-healing angiogenesis

Angiogenesis, the process by which new blood capillaries grow into a tissue from surrounding parent vessels, is a key event in dermal wound healing, malignant-tumour growth, and other pathologic conditions. In wound healing, new capillaries deliver vital metabolites such as amino acids and oxygen to the cells in the wound which are involved in a complex sequence of repair processes. The key cellular constituents of these new capillaries are endothelial cells: their interactions with soluble biochemical and insoluble extracellular matrix (ECM) proteins have been well documented recently, although the biological mechanisms underlying wound-healing angiogenesis are incompletely understood. Considerable recent research, including some continuum mathematical models, have focused on the interactions between endothelial cells and soluble regulators (such as growth factors). In this work, a similar modelling framework is used to investigate the roles of the insoluble ECM substrate, of which collagen is the predominant macromolecular protein. Our model consists of a partial differential equation for the endothelial-cell density (as a function of position and time) coupled to an ordinary differential equation for the ECM density. The ECM is assumed to regulate cell movement (both random and directed) and proliferation, whereas the cells synthesize and degrade the ECM. Analysis and numerical solutions of these equations highlights the roles of these processes in wound-healing angiogenesis. A nonstandard approximation analysis yields insight into the travel ling-wave structure of the system. The model is extended to two spatial dimensions (parallel and perpendicular to the plane of the skin), for which numerical simulations are presented. The model predicts that ECM-mediated random motility and cell proliferation are key processes which drive angiogenesis and that the details of the functional dependence of these processes on the ECM density, together with the rate of ECM remodelling, determine the qualitative nature of the angiogenic response. These predictions are experimentally testable, and they may lead towards a greater understanding of the biological mechanisms involved in wound-healing angiogenesis

Spectroscopic Observations of Optically Selected Clusters of Galaxies from the Palomar Distant Cluster Survey

We have conducted a redshift survey of sixteen cluster candidates from the Palomar Distant Cluster Survey (PDCS) to determine both the density of PDCS clusters and the accuracy of the estimated redshifts presented in the PDCS catalog (Postman et. al. 1996). We find that the matched-filter redshift estimate presented in the PDCS has an error sigma_z = 0.06 in the redshift range 0.1 < z < 0.35 based on eight cluster candidates with three or more concordant galaxy redshifts. We measure the low redshift (0.1 < z < 0.35) space density of PDCS clusters to be 31.3^{+30.5}_{-17.1} * E-06 h^3 Mpc^-3 (68% confidence limits for a Poisson distribution) for Richness Class 1 systems. We find a tentative space density of 10.4^{+23.4}_{-8.4}* E-06 h^3 Mpc^-3 for Richness Class 2 clusters. These densities compare favorably with those found for the whole of the PDCS and support the finding that the space density of clusters in the PDCS is a factor of ~5 above that of clusters in the Abell catalog (Abell 1958; Abell, Corwin, and Olowin 1989). These new space density measurements were derived as independently as possible from the original PDCS analysis and therefore, demonstrate the robustness of the original work. Based on our survey, we conclude that the PDCS matched-filter algorithm is successful in detecting real clusters and in estimating their true redshifts in the redshift range we surveyed.Comment: 23 pages with 4 figures and 3 seperate tables. To be published in the November Issue of the Astronomical Journa

Airloads research study. Volume 2: Airload coefficients derived from wind tunnel data

The development of B-1 aircraft rigid wind tunnel data for use in subsequent tasks of the Airloads Research Study is described. Data from the Rockwell International external structural loads data bank were used to generate coefficients of rigid airload shear, bending moment, and torsion at specific component reference stations or both symmetric and asymmetric loadings. Component stations include the movable wing, horizontal and vertical stabilizers, and forward and aft fuselages. The coefficient data cover a Mach number range from 0.7 to 2.2 for a wing sweep position of 67.5 degree

Airloads research study. Volume 1: Flight test loads acquisition

The acquisition of B-1 aircraft flight loads data for use in subsequent tasks of the Airloads Research Study is described. The basic intent is to utilize data acquired during B-1 aircraft tests, analyze these data beyond the scope of Air Force requirements, and prepare research reports that will add to the technology base for future large flexible aircraft. Flight test data obtained during the airloads survey program included condition-describing parameters, surface pressures, strain gage outputs, and loads derived from pressure and strain gauges. Descriptions of the instrumentation, data processing, and flight load survey program are included. Data from windup-turn and steady yaw maneuvers cover a Mach number range from 0.7 to 2.0 for a wing sweep position of 67.5 deg

Delta Self-Consistent Field as a method to obtain potential energy surfaces of excited molecules on surfaces

We present a modification of the $\Delta$SCF method of calculating energies of excited states, in order to make it applicable to resonance calculations of molecules adsorbed on metal surfaces, where the molecular orbitals are highly hybridized. The $\Delta$SCF approximation is a density functional method closely resembling standard density functional theory (DFT), the only difference being that in $\Delta$SCF one or more electrons are placed in higher lying Kohn-Sham orbitals, instead of placing all electrons in the lowest possible orbitals as one does when calculating the ground state energy within standard DFT. We extend the $\Delta$SCF method by allowing excited electrons to occupy orbitals which are linear combinations of Kohn-Sham orbitals. With this extra freedom it is possible to place charge locally on adsorbed molecules in the calculations, such that resonance energies can be estimated. The method is applied to N$_2$, CO and NO adsorbed on different metallic surfaces and compared to ordinary $\Delta$SCF without our modification, spatially constrained DFT and inverse-photoemission spectroscopy (IPES) measurements. This comparison shows that the modified $\Delta$SCF method gives results in close agreement with experiment, significantly closer than the comparable methods. For N$_2$ adsorbed on ruthenium (0001) we map out a 2-dimensional part of the potential energy surfaces in the ground state and the 2$\pi$-resonance. Finally we compare the $\Delta$SCF approach on gas-phase N$_2$ and CO, to higher accuracy methods. Excitation energies are approximated with accuracy close to that of time-dependent density functional theory, and we see very good agreement in the minimum shift of the potential energy surfaces in the excited state compared to the ground state.Comment: 11 pages, 7 figure

GaAs solar cells for laser power beaming

Efforts to develop GaAs solar cells for coupling to laser beams in the wavelength range of 800 to 840 nm are described. This work was motivated primarily by interests in space-tp-space power beaming applications. In particular, the Battelle Pacific Northwest Laboratories is conducting studies of the utilization of power beaming for several future space missions. Modeling calculations of GaAs cell performance were carried out using PC-1D to determine an appropriate design for a p/n cell structure. Epitaxial wafers were grown by MOCVD and cells fabricated at WSU Tri-Cities. Under simulated conditions, an efficiency of 53 percent was achieved for a cell coupled to 806 nm light at 400 mW/sq cm

Coupled response of membrane hydration with oscillating metabolism in live cells: An alternative way to modulate structural aspects of biological membranes?

We propose that active metabolic processes may regulate structural changes in biological membranes via the physical state of cell water. This proposition is based on recent results obtained from our group in yeast cells displaying glycolytic oscillations, where we demonstrated that there is a tight coupling between the oscillatory behavior of glycolytic metabolites (ATP, NADH) and the extent of the dipolar relaxation of intracellular water, which oscillates synchronously. The mechanism we suggest involves the active participation of a polarized intracellular water network whose degree of polarization is dynamically modulated by temporal ATP fluctuations caused by metabolism with intervention of a functional cytoskeleton, as conceived in the long overlooked association-induction hypothesis (AIH) of Gilbert Ling. Our results show that the polarized state of intracellular water can be propagated from the cytosol to regions containing membranes. Since changes in the extent of the polarization of water impinge on its chemical activity, we hypothesize that metabolism dynamically controls the local structure of cellular membranes via lyotropic effects. This hypothesis offers an alternative way to interpret membrane related phenomena (e.g., changes in local curvature pertinent to endo/exocytosis or dynamical changes in membranous organelle structure, among others) by integrating relevant but mostly overlooked physicochemical characteristics of the cellular milieu.Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Stock, Roberto P.. International and Interdisciplinary Research Network; DinamarcaFil: Olsen, Lars F.. University Of Southern Denmark; Dinamarc

A Dramatic Decrease in Carbon Star Formation in M31

We analyze resolved stellar near-infrared photometry of 21 HST fields in M31 to constrain the impact of metallicity on the formation of carbon stars. Observations of nearby galaxies show that the carbon stars are increasingly rare at higher metallicity. Models indicate that carbon star formation efficiency drops due to the decrease in dredge-up efficiency in metal-rich thermally-pulsing Asymptotic Giant Branch (TP-AGB) stars, coupled to a higher initial abundance of oxygen. However, while models predict a metallicity ceiling above which carbon stars cannot form, previous observations have not yet pinpointed this limit. Our new observations reliably separate carbon stars from M-type TP-AGB stars across 2.6-13.7 kpc of M31's metal-rich disk using HST WFC3/IR medium-band filters. We find that the ratio of C to M stars (C/M) decreases more rapidly than extrapolations of observations in more metal-poor galaxies, resulting in a C/M that is too low by more than a factor of 10 in the innermost fields and indicating a dramatic decline in C star formation efficiency at metallicities higher than [M/H] $\approx$ -0.1 dex. The metallicity ceiling remains undetected, but must occur at metallicities higher than what is measured in M31's inner disk ([M/H] $\gtrsim$ +0.06 dex).Comment: 16 pages, 13 Figures; text clarifications in response to the referee. Results are unchanged; accepted for publication in Ap

HST - WFPC2 photometry of the globular cluster ngc 288: binary systems, blue stragglers and very blue stars

We report on new WFPC2 observations of the globular cluster NGC 288, focusing our attention on peculiar stars. A very pronounced binary sequence, paralleling the ordinary Main Sequence (MS) is clearly observed in the Color Magnitude Diagram (CMD) and a huge relative fraction of Blue Straggler Stars is measured. The dataset offers the opportunity of studying the evolution of a large population of binaries (and binary evolution by-products) in a low density environment, where the evolution of such systems is not dominated by collisions and encounters. Three (very) Extreme Horizontal Branch Stars have been found, all lying outside of the cluster core.Comment: 6 pages, 3 figures, in press in the chemical evolution of the Milky Way: stars versus clusters, F. Matteucci and F. Giovannelli eds, Kluwe

VLT and NTT Observations of Two EIS Cluster Candidates. Detection of the Early-Type Galaxies Sequence at z~1

Optical data from the ESO VLT-UT1 Science Verification observations are combined with near-infrared data from SOFI at the NTT to obtain optical-infrared color-magnitude diagrams for the objects in the fields of two EIS cluster candidates. In both cases, evidence is found for a well-defined sequence of red galaxies that appear to be significantly more clustered than the background population. These results suggest that the two systems are real physical associations. The (R-Ks), (I-Ks) and (J-Ks) colors of the red sequences are used, in conjunction with similar data for spectroscopically confirmed clusters, to obtain redshift estimates of z ~ 0.9 and z ~ 1.0 for these two systems. These results make these EIS cluster candidates prime targets for follow-up spectroscopic observations to confirm their reality and to measure more accurately their redshift.Comment: 6 pages, 5 figures, to appear in Astronomy & Astrophysics (Special Letters Edition on "First Science with the VLT"