Aquaporins 7 and 11 in boar spermatozoa : detection, localisation and relationship with sperm quality

Aquaporins (AQPs) are integral membrane water channels that allow transport of water and/or small solutes across cell membranes. Although water permeability is known to play a critical role in mammalian cells, including spermatozoa, little is known about their localisation in boar spermatozoa. Against this background, two different aquaporins, AQP7 and AQP11, were identified in boar spermatozoa by Wwestern blotting and localised through immunocytochemistry analyses. WOur western blot results showed that boar spermatozoa present AQP7 (25KDa) and AQP11 (50KDa). Immunocytochemistry analyses demonstrated that AQP7 is localised at the connecting piece of boar spermatozoa, while AQP11 was found in the head and in the midpiece, and a diffuse labelling was also seen along the tail. Despite differences in AQP7- and AQP11-content being seen between boar ejaculates, these differences were not found to be correlated with sperm quality in the case of AQP7. Conversely, AQP11-content showed a significant correlation (P<0.05) with sperm quality parameters, including sperm membrane integrity and fluidity, and sperm motility. In conclusion, boar spermatozoa present AQP7 and AQP11,. Additionally, and the amounts of the latterAQP11 but not of AQP7 the former are correlated with sperm motility and membrane integrity

Influence of the sociocultural perspective on the sensory perception of wine consumers in Mexico and Spain

The increasing globalization of companies and markets, including the wine market, makes this study important as a cultural comparison between the sensory perception of wine in Mexico and Spain. Eighty consumers were selected with different consumption habits, and hedonic (Acceptance and Simple Preference) and descriptive (Word Association Task and the Check-All-That-Apply (CATA) method) sensory tests were performed. The results revealed that there were differences in the conceptualization of wine in the Word Association Task. Both populations preferred wines of Spanish origin over those of Mexican origin, especially in the case of red wine.Postprint (published version

Aquaporins 7 and 11 in boar spermatozoa : detection, localisation and relationship with sperm quality

Aquaporins (AQPs) are integral membrane water channels that allow transport of water and/or small solutes across cell membranes. Although water permeability is known to play a critical role in mammalian cells, including spermatozoa, little is known about their localisation in boar spermatozoa. Against this background, two different aquaporins, AQP7 and AQP11, were identified in boar spermatozoa by Wwestern blotting and localised through immunocytochemistry analyses. WOur western blot results showed that boar spermatozoa present AQP7 (25KDa) and AQP11 (50KDa). Immunocytochemistry analyses demonstrated that AQP7 is localised at the connecting piece of boar spermatozoa, while AQP11 was found in the head and in the midpiece, and a diffuse labelling was also seen along the tail. Despite differences in AQP7- and AQP11-content being seen between boar ejaculates, these differences were not found to be correlated with sperm quality in the case of AQP7. Conversely, AQP11-content showed a significant correlation (P<0.05) with sperm quality parameters, including sperm membrane integrity and fluidity, and sperm motility. In conclusion, boar spermatozoa present AQP7 and AQP11,. Additionally, and the amounts of the latterAQP11 but not of AQP7 the former are correlated with sperm motility and membrane integrity

Aquaporins 7 and 11 in boar spermatozoa: Detection, localisation and relationship with sperm quality

Aquaporins (AQPs) are integral membrane water channels that allow transport of water and small solutes across cell membranes. Although water permeability is known to play a critical role in mammalian cells, including spermatozoa, little is known about their localisation in boar spermatozoa. Two aquaporins, AQP7 and AQP11, in boar spermatozoa were identified by western blotting and localised through immunocytochemistry analyses. Western blot results showed that boar spermatozoa expressed AQP7 (25kDa) and AQP11 (50kDa). Immunocytochemistry analyses demonstrated that AQP7 was localised in the connecting piece of boar spermatozoa, while AQP11 was found in the head and mid-piece and diffuse labelling was also seen along the tail. Despite differences in AQP7 and AQP11 content between boar ejaculates, these differences were not found to be correlated with sperm quality in the case of AQP7. Conversely, AQP11 content showed a significant correlation (P < 0.05) with sperm membrane integrity and fluidity and sperm motility. In conclusion, boar spermatozoa express AQP7 and AQP11, and the amounts of AQP11 but not those of AQP7 are correlated with sperm motility and membrane integri

Relationship of aquaporins 3 (AQP3), 7 (AQP7), and 11 (AQP11) with boar sperm resilience to withstand freeze-thawing procedures

Cryopreservation is the most suitable method to preserve boar spermatozoa over long-term storage. However, freeze-thawing protocols inflict extensive damage to sperm cells, reducing their viability and compromising their fertilizing ability. In addition, high individual variability is known to exist between boar ejaculates, which may be classified as of good (GFE) or poor (PFE) freezability. While conventional spermiogram parameters fail to predict sperm cryotolerance in fresh spermatozoa, high levels of certain proteins, also known as freezability markers, have been found to be related to the sperm resilience to withstand freeze-thawing procedures. In this context, the hypothesis of this study was that aquaporins AQP3, AQP7, and AQP11 could be linked to boar sperm cryotolerance. Twenty-nine ejaculates were evaluated and subsequently classified as GFE or PFE based upon their sperm viability and motility at post-thawing. Fourteen ejaculates resulted to be GFE, whereas the other fifteen were found to be PFE. Relative abundances of AQP3, AQP7, and AQP11 and their localization patterns were evaluated in all fresh and frozen-thawed ejaculates through immunoblotting and immunocytochemistry. Prior to cryopreservation, relative amounts of AQP3 and AQP7 were found to be significantly (p 0.05) between freezability groups were found for AQP11, despite GFE tending to present higher levels of this protein. The localization of AQP7, but not that of AQP3 or AQP11, was observed to be affected by cryopreservation procedures. In conclusion, these results suggest that AQP3 and AQP7 are related to boar sperm cryotolerance and may be used as freezability markersThe authors acknowledge the support from Ministry of Economy and Competitiveness, Spain (Grants: AGL2013-47798-P and RYC-2014-15581

Stress neuropeptide levels in adults with chest pain due to coronary artery disease: potential implications for clinical assessment

: Substance P (SP) and neuropeptide Y (NPY) are neuropeptides involved in nociception. The study of biochemical markers of pain in communicating critically ill coronary patients may provide insight for pain assessment and management in critical care. Purpose of the study was to to explore potential associations between plasma neuropeptide levels and reported pain intensity in coronary critical care adults, in order to test the reliability of SP measurements for objective pain assessment in critical care