Gastric cancer extracellular vesicles tune the migration and invasion of epithelial and mesenchymal cells in a histotype-dependent manner

Extracellular vesicles (EVs) secreted by tumor cells modulate recipient cells’ behavior, but their effects in normal cells from the tumor microenvironment remain poorly known. In this study, we dissected the functional impact of gastric cancer cell-derived EVs (GC-EVs), representative of distinct GC histotypes, on the behavior of normal isogenic epithelial and mesenchymal cells. GC-EVs were isolated by differential centrifugation and characterized by transmission electron microscopy, nanoparticle tracking analysis, and imaging flow-cytometry. Epithelial and mesenchymal cells were challenged with GC-EVs and submitted to proliferation, migration, and invasion assays. Expression of epithelial and mesenchymal markers was followed by immunofluorescence and flow-cytometry. Our results indicated that GC-EVs secreted by diffuse-type cancer cells decrease the migration of recipient cells. This effect was more prominent and persistent for mesenchymal recipient cells, which also increased Fibronectin expression in response to EVs. GC-EVs secreted by cancer cells derived from tumors with an intestinal component increased invasion of recipient epithelial cells, without changes in EMT markers. In summary, this study demonstrated that GC-EVs modulate the migration and invasion of epithelial and mesenchymal cells from the tumor microenvironment, in a histotype-dependent manner, highlighting new features of intestinal and diffuse-type GC cells, which may help explaining differential metastasis patterns and aggressiveness of GC histotypes.Ipatimup integrate the i3S Research Unit, which is partially supported by FCT, the Portuguese Foundation for Science and Technology. This work was supported by (1) FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through Portuguese Science and Technology Foundation (FCT)/Ministério da Ciência, Tecnologia e Inovação in the framework of the project “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274); (2) the project NORTE-01-0145-FEDER-000029, supported by Norte Portugal Regional Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); (3) Portuguese FCT with the Grant Reference PTDC/BBB-ECT/2518/2014; (4) FCT PhD Programmes and by Programa Operacional Potencial Humano (POCH), specifically by the BiotechHealth Programe (Doctoral Programme on Cellular and Molecular Biotechnology Applied to Health Sciences); (5) Ipatimup Board of Directors: Project “The role of gastric cancer cell-derived extracellular vesicles”; and (6) FCT fellowships PD/BD/128406/2017 to SR, SFRH/BPD/86543/2012 to JC, and SFRH/BPD/89764/2012 to PO

Angiogenesis and apatinib: a new hope for patients with advanced gastric cancer?

Amgen; Pierre Fabreinfo:eu-repo/semantics/publishedVersio

ADENOMA CANALICULAR EM LÁBIO SUPERIOR: RELATO DE CASO

Fotografies aèries de la província de Barcelona, escala 1:5 0001:5 00

OS FEEDBACKS SOB O OLHAR DO ALUNO NA UNIVERSIDADE: ESTRATÉGIAS PARA O DESENVOLVIMENTO DO LETRAMENTO ACADÊMICO

No processo de ensino e aprendizagem, várias estratégias de leitura e escrita são fundamentais para que o desenvolvimento acadêmico se torne significativo para os sujeitos envolvidos. Dentro dessas estratégias, acreditamos que os feedbacks disponibilizados pelos docentes são instrumentos fundamentais para que os educandos tenham um crescimento acadêmico efetivo. Nesse contexto, a presente pesquisa é de cunho qualitativo e se insere no campo da Linguística Aplicada, com o objetivo de investigar as representações que os alunos do oitavo período do curso de Letras/Português, de uma universidade pública no estado do Tocantins, têm acerca da importância dos feedbacks na constituição do letramento acadêmico. Destarte, o feedback escrito é um instrumento didático que o professor pode utilizar para acompanhar e orientar os seus alunos durante o processo de ensino e aprendizagem. A partir desta pesquisa, podemos confirmar e reforçar a importância deste instrumento no desenvolvimento do letramento acadêmico dos universitários. Tal instrumento, no discurso dos participantes, demonstrou ser uma ferramenta potencializadora do modelo de Letramento Acadêmico (LEA; STREET, 2014). Além disso, ficou evidenciado como o papel do professor é indispensável para o desenvolvimento crítico e responsivo do aluno na Universidade.PALAVRAS-CHAVE: Feedback. Letramento acadêmico. Aprendizagem significativa.DOI: https://doi.org/10.47295/mgren.v7i1.156

Vitamin C and Vitamin E in Prevention of Nonalcoholic Fatty Liver Disease (NAFLD) in Choline Deficient Diet Fed Rats

AIM: Oxidative stress has been implicated in the pathogenesis of Nonalcoholic Fatty Liver Disease (NAFLD). Vitamin C and vitamin E are known to react with reactive oxygen species (ROS) blocking the propagation of radical reactions in a wide range of oxidative stress situations. The potential therapeutic efficacy of antioxidants in NAFLD is unknown. The aim of this study was to evaluate the role of antioxidant drugs (vitamin C or vitamin E) in its prevention. METHODS: Fatty liver disease was induced in Wistar rats by choline-deficient diet for four weeks. The rats were randomly assigned to receive vitamin E (n = 6) – (200 mg/day), vitamin C (n = 6) (30 mg/Kg/day) or vehicle orally. RESULTS: In the vehicle and vitamin E-treated rats, there were moderate macro and microvesicular fatty changes in periportal area without inflammatory infiltrate or fibrosis. Scharlach stain that used for a more precise identification of fatty change was strong positive. With vitamin C, there was marked decrease in histological alterations. Essentially, there was no liver steatosis, only hepatocellular ballooning. Scharlach stain was negative. The lucigenin-enhanced luminescence was reduced with vitamin C (1080 ± 330 cpm/mg/minx10(3)) as compared to those Vitamin E and control (2247 ± 790; 2020 ± 407 cpm/mg/minx10(3), respectively) (p < 0.05). Serum levels of aminotransferases were unaltered by vitamin C or vitamin E. CONCLUSIONS: 1) Vitamin C reduced oxidative stress and markedly inhibited the development of experimental liver steatosis induced by choline-deficient diet ; 2)Vitamin E neither prevented the development of fatty liver nor reduced the oxidative stress in this model