Uma Experiência Pioneira em Ensino de Ciências da Saúde via Internet na Marinha do Brasil

RESUMO O presente artigo apresenta a estrutura e avalia um curso de Suporte Básico de Vida de ensino a distância, desenvolvido pela Escola de Saúde do Hospital Naval Marcílio Dias da  Marinha do Brasil. Através da técnica da análise do discurso, cinco Sargentos Enfermeiros,  profissionais de enfermagem de nível médio, que participaram do curso foram escolhidos aleatoriamente para responder entrevistas semiestruturadas para obter informações acerca da percepção deles sobre o curso, assim como o mesmo influenciou a prática  desses profissionais de Saúde. Nesta etapa, foram utilizados  a análise de discurso para interpretação de dados, onde verificou-se que a utilização de recursos tecnológicos por meio da rede mundial de computadores, veio facilitar o acesso ao conhecimento desses militares, principalmente,  dos que se encontram distantes de pólos educacionais presenciais. O Curso “Suporte Básico de Vida à Distância”, via web, é pioneiro nas Forças Armadas nesta modalidade, atendendo não só aos militares locais, como também àqueles lotados em unidades de outros estados. A importância desse curso  se deve por ser  utilizado até os dias atuais,  com a intenção em manter profissionais militares preparados para atuação em situações que exijam conhecimento sobre o atendimento às vítimas com risco de vida, operando em missões que envolvam situações de emergência e  exijam padrões internacionais de atendimento a essas vítimas. Palavras-chave: Ensino à Distância. Internet na Educação. Educação

A new peptide from Jatropha curcas seeds: Unusual sequence and insights into its synthetic analogue that enhances proteolytic activity of papain

AbstractA new peptide (1341g/mol) from Jatropha curcas seeds was isolated. The linear sequence (APTLSGGSVPRDAD) was deduced by de novo peptide sequencing, and further used as scaffold for synthesis of linear (1342g/mol) and cyclic (1324g/mol) synthetic analogues. The full peptide sequence was identified as inserted in a putative conserved domain of late-embryogenesis proteins which produced a significant alignment hit (100% of identity and E-value of 1e−05) with a hypothetical protein JCGZ_12502 of J. curcas. Whereas in the linear peptide predominated the double charged ion state (m/z 671.68), in the cyclic form was observed the mono charged ion state (m/z of 1325.19) and an unusual MS/MS fragmentation pattern. The differences between the forms were discrete in terms of ionic mobility, retention time (reverse phase) and net charge as function of pH. Circular dichroism spectra presented an intense negative peak at 198nm which is assigned for its disordered contents. A negative peak at 222nm in the spectrum of the circular form suggested its structure was not as disordered as the linear form. The peptides were neither haemolytic nor cytotoxic and did not inhibit phytopathogenic fungi. Surprisingly, the circular but not the linear peptide increased the proteolytic activity of papain

Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines

Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of T. guianensis was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of T. guianensis extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy.The authors acknowledge CNPq, FAPEMIG (APQ-00953-12), Ministerio da Ciencia, Tecnologia e Inovacao-MCTI, and Fundo Nacional de Saude-FNS (1302/13), FINEP (MCTI/FINEP/MS/ SCTIE/DECIT-01/2013-FPXII-BIOPLAT) and CAPES (Brazilian agencies) for the financial support of this study. We thank Arali Aparecida Costa Araujo for her support in collecting of T. guianensis and Joao Maximo De Siqueira (UFSJ) and Paulo Cezar Vieira (UFSCar) for their support in the phytochemical analysis.info:eu-repo/semantics/publishedVersio

Developing an electrical power system of a mobile electron beam accelerator to treat wastewater and industrial effluents / Desenvolvimento de um sistema de energia elétrica de um acelerador móvel de feixe de elétrons para tratar águas residuais e efluentes industriais

The treatment of wastewater and industrial effluents by electron beam irradiation is a promising technique, however, not very widespread in Brazilian territory. The design and construction of a mobile unit by the Nuclear and Energy Research Institute, containing an electron beam accelerator of 700 keV and 20 kW is innovative to demonstrate the effects and positive results of this technology. The aim is to transfer the mobile unit to several companies with interest in liquid waste treatment, connect to the industry electrical system and start the ionization treatment process through electron beam. The mobile unit connection to the local electrical system may be a challenge due to the great diversity of voltages and distances involved, as well as the large injections of harmonic content generated by the electron beam accelerator that can affect sensitive loads in the industrial system. In this work, an analysis of the electrical power system of the mobile unit was made,    regarding the interruption capacity, selectivity protection and adequate short circuit levels, in order to assure a greater reliability in the operation. At the end, the control panel of the mobile unit, simulations and measurements were carried out at the 1.5 MeV and 37.5 kW electron beam accelerator, installed in the Radiation Technology Center, demonstrating the necessity of applying a filter to reduce the measured harmonic distortion. The analysis of the mobile unit electrical power system was made, in order to assure a greater reliability in the operation.

Effects of Andiroba oil (Carapa guianensis) on wound healing in alloxan-diabetic rats

Purpose: To evaluate wound healing in diabetic rats by using topic Andiroba oil (Carapa guianensis). Methods: Six male, adult, Wistar rats were distributed into three groups: Sham group (wound treatment with distilled water); Collagenase group (treatment with collagenase ointment); and Andiroba group (wound treatment with Andiroba oil). The wound was evaluated considering the macroscopic and microscopic parameters. Results: The results indicated differences in the healing of incisional wounds between treatments when compared to control group. Accelerated wound healing was observed in the group treated with Andiroba oil and Collagenase in comparison to control group, especially after the 14th day. Morphometric data confirmed the structural findings. Conclusion: There was significant effect in topical application of Andiroba oil on wound healing in rats with induced diabetes.   Keywords: Medicinal plants. Diabetes Mellitus. Wound healing. Rats

Brazilian consensus on the treatment of fibromyalgia

UNIFESP Ambulatório de FibromialgiaUFPR HC ambulatório de fibromialgiaUNIFESPUNIFESP Setor de reumatismos de partes molesPUC-SP Departamento de MedicinaPUC-Campinas Hospital Universitário Serviço de ReumatologiaSociedade Brasileira de ReumatologiaSanta Casa de Belo Horizonte Ambulatório de Fibromialgia Programa de Residência Médica em ReumatologiaFMUSP HC Serviço de ReumatologiaSanta Casa de Campo Grande Setor de Reumatologia programa de Residência em Clínica MédicaUniversidade Federal de Ciências da Saúde de Porto AlegreUNiSULUniversidade Federal do Espírito Santo Hospital Universitário serviço de ReumatologiaSociedade Brasileira de Clínica MédicaSociedade Brasileira para o Estudo da DorAssociação Brasileira de Medicina Física e ReabilitaçãoUniversidade de São Paulo Faculdade de MedicinaUniversidade Federal FluminenseAcademia Brasileira de Neurologia Departamento de DorEuropean Neurological Society Subcomitê de DorPeripheral Nerve SocietyFMUSP Grupo de MãoSociedade Brasileira de ortopedia e TraumatologiaAxia.Bio farmacoeconomia e pesquisa em saúdeUNIFESP Núcleo de Gestão de PesquisasUNIFESP, Ambulatório de FibromialgiaUNIFESP, Setor de reumatismos de partes molesUNIFESP, Núcleo de Gestão de PesquisasSciEL