Criteria For Analyzing The Quality Estimate Shake Using Two Different Impellers, "rushton Turbine" And "pbt 45"

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The Computational Fluid Dynamics (CFD) is used to numerically simulate the behavior of fluid flow, and all the laws that rule the study of fluids, both the energy and mass transfer, chemical reactions, hydraulic behavior, and other applications. Among these innumerable applications, the local behavior of a mixing tank can be estimated using the CFD techniques. In order to follow-up studies in the literature giving them increased credibility, this project aimed to determine whether both the scheduling Potency/Volume and Torque/Volume maintain the necessary level of agitation in the mix tank to extrapolate the volume 10 times and 100 times, using an axial impeller blades inclined at 45 degrees type (PBT 45), and a radial impeller (Rushton turbine) with the use of the CFD. With the results, was analyzed if a criterion is superior to the other in terms of maintaining the level of agitation in scheduling. The case study made it possible to achieve the main objective, deepen studies in Computational Fluid Dynamics. Therefore, managed to the largest domain in tools offered by CFX-ANSYS 14.0 software, allowing future work to be conducted. © Published under licence by IOP Publishing Ltd.73812012/12296-9, FAPESP, Fundação de Amparo à Pesquisa do Estado de São Paulo2012/12298-1, FAPESP, Fundação de Amparo à Pesquisa do Estado de São PauloFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)5th International Conference on Mathematical Modeling in Physical Sciences, IC-MSquare 201623 May 2016 through 26 May 201612370

Which mechanism underlies the water-like anomalies in core-softened potentials?

Using molecular dynamics simulations we investigate the thermodynamic of particles interacting with a continuous and a discrete versions of a core-softened (CS) intermolecular potential composed by a repulsive shoulder. Dynamic and structural properties are also analyzed by the simulations. We show that in the continuous version of the CS potential the density at constant pressure has a maximum for a certain temperature. Similarly the diffusion constant, $D$, at a constant temperature has a maximum at a density $\rho_{\mathrm{max}}$ and a minimum at a density $\rho_{\mathrm{min}}<\rho_{\mathrm{max}}$, and structural properties are also anomalous. For the discrete CS potential none of these anomalies are observed. The absence of anomalies in the discrete case and its presence in the continuous CS potential are discussed in the framework of the excess entropy.Comment: 8 page

Non-adiabatic collapse of a quasi-spherical radiating star

A model is proposed of a collapsing quasi-spherical radiating star with matter content as shear-free isotropic fluid undergoing radial heat-flow with outgoing radiation. To describe the radiation of the system, we have considered both plane symmetric and spherical Vaidya solutions. Physical conditions and thermodynamical relations are studied using local conservation of momentum and surface red-shift. We have found that for existence of radiation on the boundary, pressure on the boundary is not necessary.Comment: 8 Latex pages, No figures, Revtex styl

A survey on genetically modified maize in foods commercialised in Portugal

Maize, the second most important genetically modified (GM) crop, has the highest number of authorised GM events for food and feed in the EU. To provide consumer’s information, labelling for food products containing more than 0.9% of GM material is demanded by the actual EU legislation. Analysis of foods is then essential to detect and quantify GM maize material and verify the compliance with labelling information. The aim of the present work was to assess the presence of GM maize in a range of processed foods commercialised in Portugal between 2007 and 2010. For this purpose, screening of GM material was carried out by qualitative PCR targeting the 35S promoter and the NOS terminator, followed by the specific detection of Bt11, MON810, Bt176, GA21, MON863, NK603, TC1507 (also known as DAS1507), DAS59122 and MIR604 events. The identified maize events were confirmed and quantified by real-time PCR with hydrolysis probes. The overall results of GMO screening were 30% for 35S promoter, 10% for NOS terminator and 25% for identified events. The most frequently detected events were MON810, TC1507 and NK603, with one sample containing GA21, while the other events were not detected in any of the analysed foods. The quantitative results suggest the need for a more severe control since 4% of the analysed foods contained more than the threshold for labelling and none of them declared the presence of GMO

Cytokines and chemokines production by mononuclear cells from parturient women after stimulation with live Toxoplasma gondii

AbstractToxoplasma gondii is an obligate intracellular parasite that can cause variable clinical symptoms or can even be asymptomatic in immunocompetent individuals. More severe symptoms are observed in immunocompromised patients and congenital transmission of the parasite has been reported. The objective of this study was to evaluate the response of peripheral blood mononuclear cells (PBMC) in parturient and non-pregnant women exposed to live tachyzoites of T. gondii strain RH or ME49. PBMC were isolated from parturient and non-pregnant women with negative or positive serology for toxoplasmosis and cultured with live tachyzoites of the two T. gondii strains for 24 h. Next, the cell culture supernatants were collected and levels of CCL2, CCL5, IL-6, IL-10, IL-12, and TNF-α produced by PBMC after tachyzoite exposure were measured. Live tachyzoite forms of T. gondii significantly inhibited the synthesis of CCL2 in seropositive parturient women, whereas a stimulatory effect on CCL5 was observed in seronegative parturient women. Cells from T. gondii-seronegative non-pregnant women produced significantly higher levels of TNF-α and IL-12, demonstrating the proinflammatory profile induced by the presence of the parasite in culture. The results suggest that the immunomodulation seen during pregnancy contributes to the development of an environment that facilitates escape of the parasite from the immune response

Adubação não radicular com duas fontes de zinco em Coffea arábica L. "mundo novo" (B.Rodr.) Choussy

A trial was carried out on an eight old coffee plantation with visible zinc problems. The plantation was situated nearly the city of Jaú (22º30'S, 48º30'W). State of São Paulo, Brazil. The soil is classified as medium texture Oxisol of low base saturation (Latossol Vermelho Amarelo - fase arenosa). The pulverization program started in november 1977, followed in march and July 1978 (heavy harvest) and ended in march and July 1979 (light harvest). Is should be mentioned that a well reconized characteristic of arábica coffe is its habit of biennial bearing, a very heavy harvest is most often followed by a light load the next year. The following treatments and amounts of chemicals per cova hole (4 trees) were tested in accordance with a random block design: 1. 1 g of zinc (zinc sulphate, 0.5%) 2. 3 g of nitrogen (urea, 1.3%) 3. 1 g of zinc + 3 g of nitrogen (zinc sulphate 0.5% + urea 1.3%) 4. 0.25 g, 0.50 g, 1.00 g, 2.00 g of zinc plus 0.75 g, 1.50 g, 3.00 g and 6.00 of nitrogen (correspondent to NZN* 15-0-0-5 as 0.75%, 1-5%, 3.0% and 6.0% by v/v). Foliar absorption data were obtained by collecting the 3rd and 4th pairs of the coffee leaves and analysed them for N, P, K, Ca, Mg, S, B, Cu, Fe, Mn, and Zn. The main results may be summarized as follows: 1. The maximum calculated yields of clean coffee were obtained by the applications of 5.84 1 of NZN (1.13%) per hectare. 2. The applications of zinc sulphate (0.5%) and urea (1.3%) together or separate did not affected the coffee bean production. 3. The applications of 15.0 1 of NZN per hectare reduced the coffee yields. 4. Leaf damages and burning symptoms were observed by the applications of urea (1.3%) plus zinc sulphate (0.5%) and larger doses than 7.5 1 of NZN per hectare. 5. Leaf tissue analysis show that the concentrations of the elements were affecred by the age of the leaves and by the yields of the coffee trees. 6. The applications of increasing doses of NZN causes an increase in the concentration of zinc, manganese and boron in the leaves and decreased the concentration in calcium and potassium the leaves. 7. The concentration of zinc in the leaves associated with the heavy harvest, in July, was 70.0 ppm.Em cafezal com 8 anos de idade, com deficiência visível de zinco, situado em um Latossol Amarelo - fase arenosa, do município de Jaú, SP, procederam-se pulverizações em novembro, março e julho de 1978 (grande safra) e março e julho de 1979 (pequena safra), O delineamento experimental foi de blocos ao acaso e constou dos seguintes tratamentos, nas doses por planta: lg de zinco (sulfato de zinco 0,5%), 3g de nitrogênio (uréia 1,3%), lg de zinco + 3g de nitrogênio (sulfato de zinco 0,5% + uréia 1,3%) e 0,25g, 0,50g, 1 ,00g e 2,00g de zinco juntamente com 0,75g, l,50g, 3,00g e 6,00g de nitrogênio (respectivamente NZN 15-0-0-5 * a 0,75%, 3,00% e 6,00% v/v). Na época das pulverizaçoes foram coletadas amostras de 3º e 4º pares de folhas, e determinados os teores de nitrogênio, fósforo, potássio, cálcio, magnésio, boro, cobre, ferro, manganês e zinco. Os resultados mostraram que: a) a produção máxima calculada de café beneficiado foi 3-009,4 kg/ha, obtida com pulverizaçao de 5,84 1 de NZN por hectare (NZN 1 ,17%) ; b) o sulfato de zinco e a uréia, juntos ou isoladamente, não afetaram a produção; c) a dose de 15,0 1 de NZN por hectare diminuiu a produção; d) as aplicações de uréia (1,3%) + sulfato de zinco (0,5%) e de doses de NZN superiores a 7,51/ha provocaram injúrias visíveis nas folhas. Esse efeitos se agravaram com dosagens de 15 1 e 30 1 de NZN por hectare; e) as concentrações dos nutrientes nas folhas foram afetadas de maneira diferente pelas épocas de amostragem e pela produção do cafeeiro; f) a aplicação de doses crescentes de NZN provocou aumento nas concentrações de zinco, manganês e boro e diminuição nas de cálcio e potássio nas folhas, em determinadas épocas da grande ou pequena safra; g) a concentração de zinco em julho, relacionada com a maior produção do cafeeiro, foi 70 ppm

Soybean DNA extraction from blended refined vegetable oils

The major genetically modified (GM) crop species is soybean (Glycine max), accounting for 53% of the total world’s GM planted area and 70% of the soybean annual production in 2008 [1]. Since the approval of Roundup Ready® (RR) soybean in EU, the production of soybean oil using GM seeds has been increasing. In EU, the doubts raised by the use of genetically modified organisms (GMO) lead to the mandatory labelling for food products containing more than 0.9% of authorised GMO. In blended edible oils prepared with mixtures of two or more different oils, it is important to verify the labelling statements concerning their constituents and the presence of GM material, since soybean oil is frequently used. The analysis of DNA coupled with polymerase chain reaction (PCR) has been the technique of choice to monitor the presence of GMO in food. However, it is very difficult to obtain amplifiable DNA from oil since most vegetable oils, like crude soybean oil, must be refined prior to its consumption

Assessing the effect of pharmaceutical excipients on the DNA extraction from plant food supplements

in the EU market as ingredients in formulations, which are sold as plant food supplements (PFS). Among the several issues that may affect the safety of PFS, the most relevant concerns aduÍterations by the illegal addition of pharmaceutical drugs and/or the swap/ misidentification ofplant material, with cases of acate toxicity already reported [l]. Owing to the high similarity and distinct therapeutic uses ofseveral medicinal plants, accurate and fast methodologies allowing their distincüon are required. For that purpose, DNA-based methods are considered fast, sensitive and highly specific tools, allowing the unequivocal identification of plant species. Up to date, most of DNA methodologies reporting the identification of plant species essentially concern medicinal plants [2], with few works being developed for the authentication of PFS.This work was supported by the projects EXPL/DTP-SAP/1438/2013 (4SaferPFS) and Pest-C/EQB/LA0006/2013 financed by FCT (Fundação para a Ciência e Tecnologia) (FEDER funds through COMPETE). Teimo J.R. Fernandes is grateful to FCT PhD grant (SFRH/BD/93711/2013) financed by POPH-QREN (subsidised by FSE and MCTES).info:eu-repo/semantics/publishedVersio

Corpus luteum blood flow evaluation on Day 21 to improve the management of embryo recipient herds

AbstractThe aim of the present study was to use blood flow evaluation of the CL at 14 days after embryo transfer to detect nonpregnant animals and optimize the management of bovine recipients. The estrous cycle was synchronized in 165 recipients, and the day of expected ovulation was considered to be Day 0. Embryo transfer was performed 7 days later, on Day 7. On Day 21, pregnancy was diagnosed on the basis of blood flow evaluation of the CL (DG21—predictive diagnostic). To validate this methodology, visual scores for blood flow were compared to objective data extracted from CL ultrasound images recorded in the Doppler mode. The size was also evaluated using recorded images of the CL in the B mode. Blood samples were also collected for further analysis of the progesterone (P4) concentration. The diagnosis of pregnancy was confirmed at 35 days after estrus (DG35—definitive diagnostic). The DG21 showed that 55.2% (90 of 163) of the animals were presumptively pregnant, and this value was higher (P < 0.04) than that obtained at DG35 (43.6%, 71 of 163). The predictive diagnostic achieved moderate specificity (79.3%) for the detection of pregnancy, but most importantly, high sensitivity (100%) for the detection of nonpregnant recipients. The overall accuracy of the diagnosis was 88.3%. The P4 concentrations were different (P < 0.02) and correlated with each visual score assigned for the CL size. Visual scores for CL blood flow were also efficient (P < 0.0001) to distinguish animals with different levels of P4; however, P4 concentrations were higher for scores 1 and 2 (high and regular blood flow, respectively) than those for score 3 (low blood flow). This technique showed high sensitivity and facilitated the early detection of nonpregnant animals. The DG21 would allow about 79.3% of nonpregnant animals to be resynchronized 9 to 14 days earlier, when compared to conventional management based on pregnancy diagnosis at Days 30 to 35

Survey Of Acarin Fauna In Dust Samplings Of Curtains In The City Of Campinas, Brazil.

The aim of this study was to investigate the mite fauna present in 33 living room and 22 bedroom curtain dust samples from 41 different homes in the southern Brazilian city of Campinas, SP. A total of 148 mite bodies were found. Of these, 83 were found in living-room curtain samples (56.1% of total) and 65 were in bedroom curtain dust samples (43.9%). The most frequently observed mite suborders were: Acaridida (n = 79; 53.4%), Actinedida (n=53; 35.8%), Oribatida (n=14; 9.5%), and Gamasida (n=2; 1.3%). The most frequent families were Pyroglyphidae (n=61; 41.2%), Eriophyidae (n=25; 16.9%), Tarsonemidae (n=15; 10.1%), and Glycyphagidae (n=13; 8.8%). No statistical difference was observed between the number of mites found in the samples from living room and bedroom curtains.651252