Mechanistic Insight into the Enzymatic Reduction of Truncated Hemoglobin N of Mycobacterium tuberculosis: role of the CD loop and pre-A Motif in electron cycling

Background: The HbN of Mycobacterium tuberculosis carries a potent nitric-oxide dioxygenase activity despite lacking a reductase domain. Results: The NADH-ferredoxin reductase system acts as an efficient partner for the reduction of HbN. Conclusion: The interactions of HbN with the reductase are modulated by its CD loop and the Pre-A region. Significance: The present study provides new insights into the mechanism of electron transfer during nitric oxide detoxification by HbN.Fil: Singh, Sandeep. Institute of Microbial Technology; IndiaFil: Thakur, Naveen. Institute of Microbial Technology; IndiaFil: Oliveira, Ana. Universidad de Barcelona; EspañaFil: Petruk, Ariel Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Hade, Mangesh Dattu. Institute of Microbial Technology; IndiaFil: Sethi, Deepti. Institute of Microbial Technology; IndiaFil: Bidon Chanal, Axel. Universidad de Barcelona; EspañaFil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Datta, H.. Institute of Microbial Technology; IndiaFil: Parkesh, R.. Institute of Microbial Technology; IndiaFil: Estrin, Dario Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Luque, F. Javier. Universidad de Barcelona; EspañaFil: Dikshit, Kanak L.. Institute of Microbial Technology; Indi

Prospectus, January 24, 1969

BLACK PANEL TALK; Fall Quarter Honors List; New Officers Sworn in at SG Meeting; SIU Visitor; Editors\u27 Column; Readerspeak; Locker Dispute; Panel Speaks Up; Black Rap; Higher Tuition: How Students React to Increase; Parkland Seen; Presenting Another Dynamic Duo; Ask Charlie Brown; Call it By Name; Hit the Ice This Mondayhttps://spark.parkland.edu/prospectus_1969/1013/thumbnail.jp

Platelet-derived exosomes induce endothelial cell apoptosis through peroxynitrite generation: experimental evidence for a novel mechanism of septic vascular dysfunction

Abstract\ud \ud \ud \ud Introduction\ud \ud Several studies link hematological dysfunction to severity of sepsis. Previously we showed that platelet-derived microparticles from septic patients induce vascular cell apoptosis through the NADPH oxidase-dependent release of superoxide. We sought to further characterize the microparticle-dependent vascular injury pathway.\ud \ud \ud \ud Methods\ud \ud During septic shock there is increased generation of thrombin, TNF-α and nitric oxide (NO). Human platelets were exposed for 1 hour to the NO donor diethylamine-NONOate (0.5 μM), lipopolysaccharide (LPS; 100 ng/ml), TNF-α (40 ng/ml), or thrombin (5 IU/ml). Microparticles were recovered through filtration and ultracentrifugation and analyzed by electron microscopy, flow cytometry or Western blotting for protein identification. Redox activity was characterized by lucigenin (5 μM) or coelenterazine (5 μM) luminescence and by 4,5-diaminofluorescein (10 mM) and 2',7'-dichlorofluorescein (10 mM) fluorescence. Endothelial cell apoptosis was detected by phosphatidylserine exposure and by measurement of caspase-3 activity with an enzyme-linked immunoassay.\ud \ud \ud \ud Results\ud \ud Size, morphology, high exposure of the tetraspanins CD9, CD63, and CD81, together with low phosphatidylserine, showed that platelets exposed to NONOate and LPS, but not to TNF-α or thrombin, generate microparticles similar to those recovered from septic patients, and characterize them as exosomes. Luminescence and fluorescence studies, and the use of specific inhibitors, revealed concomitant superoxide and NO generation. Western blots showed the presence of NO synthase II (but not isoforms I or III) and of the NADPH oxidase subunits p22phox, protein disulfide isomerase and Nox. Endothelial cells exposed to the exosomes underwent apoptosis and caspase-3 activation, which were inhibited by NO synthase inhibitors or by a superoxide dismutase mimetic and totally blocked by urate (1 mM), suggesting a role for the peroxynitrite radical. None of these redox properties and proapoptotic effects was evident in microparticles recovered from platelets exposed to thrombin or TNF-α.\ud \ud \ud \ud Conclusion\ud \ud We showed that, in sepsis, NO and bacterial elements are responsible for type-specific platelet-derived exosome generation. Those exosomes have an active role in vascular signaling as redox-active particles that can induce endothelial cell caspase-3 activation and apoptosis by generating superoxide, NO and peroxynitrite. Thus, exosomes must be considered for further developments in understanding and treating vascular dysfunction in sepsis.LRL and MJ have research grants from Fundação de Amparo a Pesquisa do Estado de São Paulo – FAPESP. MJ received a research grant from Sociedade Beneficente Israelita-Brasileira Hospital Albert Einstein.LRL and MJ have research grants from Fundação de Amparo a Pesquisa do Estado de São Paulo – FAPESP. MJ received a research grant from Sociedade Beneficente IsraelitaBrasileira Hospital Albert Einstein

Prospectus, April 28, 1969

CAGERS REWARDED AT BANQUET; Dean\u27s Team; Parkland Tops; Black Rap; Readerspeak; Grey Is The Way; BSA; Quote Of The Week; PC Board Elects; Joel Fort; Just Ask Minerva!; Registrations ; Scholarships Available; To Mel; Spring Fine Arts Exhibit; Veterans\u27 Dinner; Auto Farm Equipment Club; Doris Larson Interview; Kenneth Armstrong: South Vietnam, An Endless Circle?; Parkland Honors First Cagers; 2 Cobras Named By Paper; Staerkel Guest Speaker, Eight Letter Winners; IM All-Stars Whip Faculty; Counselors\u27 Corner...; ED. Committee Approves Lawhttps://spark.parkland.edu/prospectus_1969/1008/thumbnail.jp

Prospectus, December 18-19, 1968

LOOKING AT TWO PRESIDENTS; Win Over Springfield Gives PC 3-0 Record; Rah, Rah, Black? White? Who Will Cheer For Us?; \u27Cosmic Rosetta Stone\u27 To Be Explored By U.S.; Prichett Hopes Speed Will Build Winner; Parkland Makes Cage Debut; Mock, Smith Lead PC Past AFB; Butler\u27s 37 Sparks Win; IM Action Continues; 5 Fall Championshttps://spark.parkland.edu/prospectus_1968/1000/thumbnail.jp

Prospectus, May 2, 1969

SG RAPS PROSPECTUS; Board Approves 2 New Programs; Board Meets Tuesday Over Student Rights; Permanent Officers Elected; Dr. Smith Resigns, Gets Doctorate; Applications Open For Art Exhibit; Editors\u27 Column; Readerspeak; Queen hopefuls Make Ready, BSA Pulls Out; Draft Counselor Opens At PC; Betas To State; Parkland\u27s Para-Professionals; Culture Abounds In Mrs. Clore\u27s Home; Gayle Wright: Won\u27t Accept Second Best; Counselors\u27 Corner...; GI\u27s Survive To Get Home; Klapperich Entered At Meet; IM Softball; Parkland\u27s Liz Sharp; New Bill Limits JC Recruiting; At PCKFA Meet: Kite Flying Instructors Battle April\u27s Breezes;https://spark.parkland.edu/prospectus_1969/1007/thumbnail.jp

Prospectus, February 7, 1969

CAMPUS HAS NATURAL LOOK -- WILL BE COMPLETED BY 1975, STUDENTS MOVE IN BY 1971; Black Rap; Readerspeak; Supersnake; Plato Hook-Up at PC; Ask Charlie Brown; SG Charters Clubs, Approves Cap, Gown; Student Guides Needed; BSA Gets New Dean; Jobs Open at Parkland; Mrs. Scott For Equality at PC; Visit From Blackburn Counselor; Feels Close to Students; Parkland Theater Now in First Production; Student Reporters Evicted From Faculty Association; Dietz, Lehnis Stop Cobras; Two Prodigals Reinstated on Basketball Team; Parkland Shoots .539 in Clipping Olney JC; Kays Forfeit, Canton Next; Druts, Lobos and Cougars Tied at 2-0; Parkland Drops 9th, Millikin Wins 102-87https://spark.parkland.edu/prospectus_1969/1012/thumbnail.jp

Prospectus, March 31, 1969

PROSPECTUS X-RAYS PC; New Clubs On Campus; Getting Too Drafty?; Urges Activities Through Media; IOC Formed; Blind; Editors\u27 Column; Black Rap; PC Polled Again: Students Ask For Culture; Literary Mag. Much-Needed Facet Of PC; Around Parkland College:; Auto-Farm Tech Club in Planning; Refresher Course, For RN\u27s; Lit Mag Finally Okayed; Scholarship For JC Students; Louis Lomax Speaks At PC; Parkland: What Is And What Will Be; Self Contained Unit; Photo Essay By Lou McClellan; Reporters Allowed To Sit With Faculty; Board Cuts Campus Cost With Clay File; Ask Minerva; 8 SG Posts Now Open; Fort To Speak; Town; A Chance To Inovate; Miss Snyder - \u27Best Decorated\u27; Counselors\u27 Corner...; Council For Interracial Projects; On April Fool\u27s Day: Faculty Vs. All-Stars; Boris Karloff is Dead, Frankenstein is Gone; Druts Win Championship; Four Sports In Spring Intramuralshttps://spark.parkland.edu/prospectus_1969/1009/thumbnail.jp

6Li direct breakup lifetimes

alpha-d coincidence data were studied for the 6Li + 59Co reaction at E(lab) = 29.6 MeV. By using a kinematic analysis, it was possible to identify which process, leading to the same final state, has the major contribution for each of the selected angular regions. Contributions of the 6Li sequential and direct breakup to the incomplete fusion/transfer process were discussed by considering the lifetimes obtained by using a semiclassical approach, for both breakup components.Comment: 5 pages, 4 figures, Invited Talk (Parrallel Sessions) of A. Szanto de Toledo, prepared for the Proccedings of the 10th International Conference on Nucleus-Nucleus Collisions, August 16-21, 2009, Beijing, China; submitted to Nucl. Phys. A (Proceedings of NN2009