Características dos principais porta-enxertos recomendados para citros no Rio Grande do Sul.

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Magnetic nanoparticles covered by or entrapped in lipid bilayers: Advances towards dual cancer therapy

In this work, both aqueous magnetoliposomes (magnetic nanoparticles entrapped in liposomes) and solid magnetoliposomes (a cluster of particles covered by a lipid bilayer) have been developed, containing either nickel/silica core/shell nanoparticles, nickel ferrite or manganese ferrite nanoparticles, with diameters below 150 nm, suitable for biomedical applications. Moreover, both aqueous (AMLs) and solid (SMLs) magnetoliposomes show a superparamagnetic behavior, the SMLs exhibiting a high saturation magnetization.Financial support by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013 and UID/QUI/00686/2016 is acknowledged. A.R.O Rodrigues thanks the FCT for SFRH/BD/90949/2012 PhD grant and funding to MAP-Fis Doctoral Program.info:eu-repo/semantics/publishedVersio

Decellularized dermal matrices: unleashing the potential in tissue engineering and regenerative medicine

Decellularized dermal matrices (dDMs) have emerged as effective biomaterials that can revolutionize regenerative medicine, particularly in the field of wound healing and tissue regeneration. Derived from animal or human skin, dDMs offer great biocompatibility, remarkable biochemistry, and a macromolecular architecture equivalent to the native tissue. Notably, among the biomimetic extracellular matrix (ECM)-based scaffolds, dDMs stand out due to their inherent dermal microenvironment, holding high value for skin regeneration and reconstructive surgery. The integration of dDMs as a biomaterial base for bioinks in advanced manufacturing technologies opens promising avenues for crafting precise, biomimetic tissue engineering (TE) constructs with optimized recellularization properties. This mini review outlines the main sources, differential decellularization techniques applied to dDMs, and their significance intissue engineering and regenerative medicine. It subsequently delves into the different categories of decellularized materials obtained, their unique physical and biochemical attributes, as well as their applications to promote wound healing and regenerating skin and soft tissues. Additionally, the currently available market products based on dDMs are examined and the main outcomes are compared. Finally, the article highlights current barriers in the field and anticipates the future challenges and applications of dDMs-based therapies.info:eu-repo/semantics/publishedVersio

Development of a self-diagnostic system integrated into a cyber-physical system

CONTROLAR provides Bosch with an intelligent functional testing machine used to test the correct functioning of the car radios produced. During this process, the radios are submitted to several tests, raising the problem of how the machine detects errors in several radios consecutively, making it impossible to know if the device has a problem since it has no module to see if it works correctly. This article arises from the need to find a solution to solve this problem, which was to develop a self-diagnostic system that will ensure the reliability and integrity of the cyber-physical system, passing a detailed state of the art. The development of this system was based on the design of an architecture that combines the KDT methodology with a DSL to manage and configure the tests to integrate the self-diagnostic test system into a CPS. A total of 28 test cases were performed to cover all its functionalities. The results show that all test cases passed. Therefore, the system meets all the proposed objectives.This article is a result of the project POCI-01-0247-FEDER-040130, supported by Operational Program for Competitiveness and Internationalization (COMPETE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF)

Allergenicity reduction of cow's milk proteins using latex peptidases

The present study evaluated four laticifer fluids as a novel source of peptidases capable of hydrolyzing proteins in cow´s milk. The latex peptidases from Calotropis procera (CpLP), Cryptostegia grandiflora (CgLP), and Carica papaya (CapLP) were able to perform total hydrolysis of caseins after 30 min at pH 6.5, as confirmed by a significant reduction in the residual antigenicity. Casein hydrolysis by Plumeria rubra latex peptidases (PrLP) was negligible. Moreover, whey proteins were more resistant to proteolysis by latex peptidases; however, heat pretreatment of the whey proteins enhanced the degree of hydrolysis and reduced the residual antigenicity of the hydrolysates. The in vivo assays show that the cow´s milk proteins hydrolysed by CgLP and CapLP exhibited no immune reactions in mice allergic to cow´s milk, similar to a commercial partially hydrolysed formula. Thus, these peptidases are promising enzymes for the development of novel hypoallergenic formulas for children with a milk allergy.Fil: Oliveira, João P. B.. Universidade Estadual do Ceará; BrasilFil: Candreva, Ángela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Rizzo, Gaston Pascual. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Ramos, Márcio V.. Universidade Estadual do Ceará; BrasilFil: Oliveira, Jefferson S.. Universidade Estadual do Ceará; BrasilFil: Oliveira, Hermógenes D.. Universidade Federal Do Piaui.; BrasilFil: Ary, Maria B.. Universidade Estadual do Ceará; BrasilFil: Docena, Guillermo H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Freitas, Cleverson D. T.. Universidade Federal Do Ceara; Brasi

Magnetic liposomes based on nickel ferrite nanoparticles as nanocarriers for new potential antitumor compounds

Guided transport of biologically active molecules (most of them toxic and with systemic side effects) to target specific sites in human body has been a focus of research in therapeutics in the past years. Magnetoliposomes (liposomes entrapping magnetic nanoparticles) are of large importance, as they can overcome many pharmacokinetics problems and can be guided and localized to the therapeutic site of interest by external magnetic field gradients [1,2]. In this work, nickel ferrite nanoparticles (NPs) with size distribution of 11±5 nm were obtained. Synthesized NPs show superparamagnetic behaviour at room temperature (magnetic squareness of 7.2×10-5 and coercivity field of 12 Oe), being suitable for biological applications. These NPs were either entrapped in liposomes, originating aqueous magnetoliposomes (AMLs), or covered with a lipid bilayer, forming dry magnetoliposomes (DMLs), the last ones prepared by a new promising route. Recently, AMLs and DMLs containing nickel-based nanoparticles were successfully prepared and characterized [3]. A potential antitumor compound [4] was successfully incorporated into the lipid bilayer of magnetoliposomes. DMLs structure was evaluated by FRET (Förster Resonance Energy Transfer) measurements between the fluorescent-labeled lipids NBD-C12-HPC (donor) included in the second lipid layer and rhodamine B DOPE (acceptor) in the first lipid layer. A FRET efficiency of 23% was calculated, with a corresponding donor-acceptor distance (r) of 3.11 nm, confirming DMLs structure. Preliminary assays of the non-specific interactions of both types of magnetoliposomes with biological membranes (modeled by giant unilamellar vesicles, GUVs) were performed, keeping in mind future applications of drug delivery using this type of magnetic systems. Membrane fusion between magnetoliposomes and GUVs was confirmed by FRET.FCT - Fundação para a Ciência e a Tecnologia, PEst-C/FIS/UI0607/2013 (F-COMP-01-0124-FEDER-022711

Magnetic/plasmonic MnFe2O4/Au nanoparticles covered with lipid bilayers for applications in thermotherapy

In this work, three different types of magnetic/plasmonic nanoparticles were prepared: core-shell nanoparticles with a manganese ferrite core and a gold shell; plasmonic gold nanoparticles decorated with magnetic nanoparticles of manganese ferrite; and magnetic nanoparticles of manganese ferrite decorated with plasmonic gold nanoparticles. The structural, spectroscopic and magnetic properties of these nanoparticles were evaluated. In order to further develop applications in cancer therapy, the prepared mixed nanoparticles were covered with a lipid bilayer. The local heating capability of these nanosystems was tested through the quenching of rhodamine fluorescence incorporated in the lipid layer.Financial support by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013. A.R.O Rodrigues thanks the FCT for SFRH/BD/90949/2012 PhD grant and funding to MAP-Fis Doctoral Program.info:eu-repo/semantics/publishedVersio

Magnetic liposomes containing calcium ferrite nanoparticles for breast cancer therapy

In this work, calcium ferrite nanoparticles were prepared and characterized. The structural and magnetic properties of the nanoparticles were evaluated by XRD, TEM and SQUID. The synthesized nanoparticles were either entrapped in liposomes, originating aqueous magnetoliposomes (AMLs), or covered with a lipid bilayer, forming solid magnetoliposomes (SMLs). Magnetoliposomes present average diameters around 150 nm, suitable for biomedical applications. The magnetoliposomes were loaded with new potential anticancer drugs, thienopyridine derivatives, with a strong antitumor activity against breast cancer cells.This work was supported by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding of CF-UM-UP (UID/FIS/04650/2013) and of CQUM (UID/QUI/00686/2013). FCT, POPH-QREN and FSE are acknowledged for the PhD grants of A. R. O. Rodrigues (SFRH/BD/90949/2012) and V. A. Machado (SFRH/BD/77373/2011) and for financial support to MAP-Fis Joint Doctoral Programme.info:eu-repo/semantics/publishedVersio

Automated design of microwave discrete tuning differential capacitance circuits in Si-integrated technologies

A genetic algorithm used to design radio-frequency binary-weighted differential switched capacitor arrays (RFDSCAs) is presented in this article. The algorithm provides a set of circuits all having the same maximum performance. This article also describes the design, implementation, and measurements results of a 0.25 lm BiCMOS 3-bit RFDSCA. The experimental results show that the circuit presents the expected performance up to 40 GHz. The similarity between the evolutionary solutions, circuit simulations, and measured results indicates that the genetic synthesis method is a very useful tool for designing optimum performance RFDSCAs