781 research outputs found

    Seasonal Variation Of The Essential Oil From Two Brazilian Native Aldama La Llave (asteraceae) Species

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    Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Aldama arenaria and A. robusta are morphologically similar aromatic species that have seasonal development. The yield and chemical composition of essential oils from aerial and underground vegetative organs of these species were compared to verify the production of volatile metabolites in flowering and dormant phases of development and to identify if there are unique compounds for either species. The major compound in the essential oils from A. arenaria leaves was palustrol (16.22%) and for aerial stems was limonene (15.3%), whereas limonene (11.16%) and alpha-pinene (19.64%) were the major compounds for leaves and aerial stems from A. robusta, respectively. The major compound for the underground organs was a-pinene, in both species and phenological stages. High amounts of diterpenes were found especially for A. arenaria essential oils. Each analyzed species presented unique compounds, which can provide a characteristic chemical profile for both species helping to solve their taxonomic problems. This study characterized for the first time the yield and essential oil composition of A. arenaria and A. robusta, which have medicinal potential, and some of the compounds in their essential oils are unique to each one and may be useful in helping the correct identification of them.883118991907Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [303715/2014-6]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/514543, 2010/02005-1]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

    Use of natural rubber membranes as support for powder TiO2 and Ag/TiO2 photocatalysts

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    Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)The purpose of this study was to synthesize TiO2-polymer composites able to act as photocatalyst membranes. TiO2 catalysts were prepared using the sol-gel method to contain 0.0, 0.5, 1.0, and 2.0 wt.% of embedded Ag particles, subsequently incorporated into natural rubber latex at a weight fraction of 15%. Samples of these ceramic powders were suspended in a latex emulsion (natural rubber), cast in Petri dishes and slowly dried in an oven. The resulting materials were evaluated by X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray, diffuse reflectance spectroscopy, differential scanning calorimetry, thermogravimetry, and photocatalytic assaying using methylene blue as an organic pollutant reference. All composite membranes exhibited good photoactivity conferred by TiO2 powder, with 98% dye fading after 300 min of ultraviolet irradiation.The purpose of this study was to synthesize TiO2-polymer composites able to act as photocatalyst membranes. TiO2 catalysts were prepared using the sol-gel method to contain 0.0, 0.5, 1.0, and 2.0 wt.% of embedded Ag particles, subsequently incorporated in273575583CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFUNDECT - FUNDAÇÃO DE APOIO AO DESENVOLVIMENTO DO ENSINO, CIÊNCIA E TECNOLOGIA DO ESTADO DE MATO GROSSO DO SUL |Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)sem informaçãosem informaçãosem informaçãosem informaçãoThe authors wish to thank the Brazilian funding agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Apoio ao Desenvolvimento do Ensino, Ciênci

    Transcription of Aspergillus nidulans pacC is modulated by alternative RNA splicing of palB

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    AbstractFungi have evolved elaborate signal transduction networks for remodeling metabolic pathways to scavenge nutrients, including the secretion of nutritional enzymes. This adaptive response involves the conserved PacC/Pal signal transduction pathway, which mediates the transcriptional response to ambient pH. In this study, we show that transcription of the gene for PacC is modulated in response to nutrient changes, phosphate and carbon sources, and pH. In addition, we show that transcription of pacC is modulated in response to alternative RNA splicing of the palB gene. These results reveal novel aspects of the complex network involved in modulation of pacC

    Melatonin reduces the severity of experimental amoebiasis

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    <p>Abstract</p> <p>Background</p> <p>Melatonin has immunomodulatory effects but very little is known about its influence in protozoan infections, such as <it>Entamoeba histolytica</it>, which causes amoebiasis, a disease with significant morbidity and mortality. In this study, we evaluated the effects of exogenous melatonin interference in experimental amoebiasis and on interactions between human blood cells and <it>E. histolytica </it>trophozoites.</p> <p>Methods</p> <p>The effect of melatonin was investigated in models of experimental amoebiasis in hamsters and rats by evaluating the area of necrosis induced by <it>E. histolytica</it>. The activity of melatonin on the interactions between leukocytes and amoebae was determined by examining leukophagocytosis. For <it>in vitro </it>tests, polymorphonuclear and mononuclear human blood leucocytes were incubated with <it>E. histolytica </it>trophozoites.</p> <p>Results</p> <p>The areas of amoebic necrosis were significantly reduced in animals treated with melatonin. Melatonin treatment increased leukophagocytosis but was associated with a greater number of dead amoebae.</p> <p>Conclusions</p> <p>These results suggest that melatonin may play a beneficial role in the control of amoebic lesions, raising the possibility that this drug may be used as an adjuvant in anti-amoebic therapy.</p

    Extended treatment with interferon and ribavirin in a hemodialysis patient with chronic hepatitis C

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    Hemodialysis patient with chronic HCV infection,who was started on monotherapy with interferon.Qualitative HCV RNA remained positive at 12 weeks of treatment; ribavirin was associated. HCV RNA was negative at week 24 and treatment was extended to 72 weeks. HCV RNA negative six months after treatment.19319

    Comparison of primary human gingival fibroblasts from an older and a young donor on the evaluation of cytotoxicity of denture adhesives

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    Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially for older adults. These materials should be biocompatible, i.e., they cannot cause undesired biological responses and be non-cytotoxic to oral tissues. However, in vitro testing of DA biocompatibility employing primary cell culture may possibly be affected by other factors, such as the donor age. Objective To compare the cytotoxicity of three different denture adhesives when assessed in primary gingival fibroblasts from a young donor or from an older donor, as well as the release of the basic fibroblast growth factor (bFGF), and the inflammatory response marker interleukin-6 (IL-6). Material and Methods Gingival fibroblasts isolated from a 30- and a 62-year-old donor were assayed for proliferation (1-7 days) and sensitivity to latex (positive control). Fibroblasts were indirectly exposed to Corega Ultra (cream), Corega powder and Fixodent Original for a 24 h period and assayed by XTT and Crystal Violet tests. The release of IL-6 and bFGF by exposed cells was determined by ELISA. Results While cells from the young donor presented higher cell growth after 7 days, the sensitivity to increasing concentrations of latex extracts was very similar between young and older cells. Both XTT and CVDE detected no difference between the DA and the control group. All materials induced higher levels of IL-6 and bFGF compared to control. Cells from the older donor exposed to Corega Ultra released lower levels of cytokine and growth factor. Conclusions All materials were considered non-cytotoxic, but affected cytokine and growth factor release. The biological differences found between fibroblasts from both donors could be due to individual or age-related factors. The authors suggest the use of cells from older donors on studies of dental products aimed at older patients, to better simulate their physiological response

    Effect of Different Numbers of Interset Antagonist Proprioceptive Neuromuscular Facilitation Stretching on the Total Number of Repetitions for the Agonists

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    International Journal of Exercise Science 15(4): 498-506, 2022. Recent studies have observed that stretching applied to antagonist muscles can promote improvement in agonist muscle performance. The purpose of this study was to investigate the effect of different numbers of interset proprioceptive neuromuscular facilitation (PNF) stretching for the antagonists on the total number of repetitions completed for the agonists (quadriceps) in the leg extension exercise. Fourteen physically active individuals (age: 29.35 ± 10.5 years; body mass: 79.1 ± 11.34 kg; height: 170.4 ± 8.7 cm) participated in this study. The following experimental protocols were performed: 1) Traditional protocol (Traditional) – without previous stretching; 2) PNF with lesser duration (PNF1—3 sets of 20 secs.); 3) PNF with greater duration (PNF2—3 sets of 30 secs.). Within the experimental protocols (PNF1 and PNF2), stretching exercises for the antagonists were performed before and between the four sets of the unilateral leg extension exercise. All tests were performed on the dominant limb only. The results showed that there was a significant difference in the total number of repetitions for the PNF2 protocol versus the Traditional protocol (p = 0.026). However, there was no significant difference between the PNF1 protocol versus the Traditional protocol (p = 0.577). In conclusion, in the leg extension exercise, an extended duration of interset PNF stretching for the hamstrings, promoted greater contractile performance for the quadriceps as demonstrated by significantly greater total repetitions over four sets

    Preparação, caracterização e propriedades de filmes poliméricos com potencial aplicação no recobrimento de sementes

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    Films of sodium carboxymethyl cellulose, sodium alginate and their mixture were evaluated in terms of interactions between the polymers, morphology, water absorption and application as seed coatings. FTIR analysis suggested that only hydrophobic interactions occurred between the polymers. The coating of bean seeds was confirmed by microscopy, indicating the formation of dense and homogeneous films with 7 &#956;m thickness. The obtained films did not affect the germination capacity of the seeds. In summary, the characteristics and properties of the films formed and the water absorption capacity, indicate that these systems are viable for use in seed coating processes
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