Evaluation of Barley Genotypes Against Spot Blotch Disease in Inner Tarai Region of Nepal

Spot blotch caused by Bipolaris sorokiniana (Sacc. in Sorok.) Shoem. is an important disease of barley (Hordeum vulgare L.). A total of 126 barley genotypes received from Hill Crops Research Program, Kabre, Dolakha having SoluUwa as a susceptible check and Bonus as a resistant check were evaluated as barley disease screening nursery (BDSN) under natural epiphytotic condition at National Maize Research Program, Rampur, Chitwan during winter seasons of 2017 and 2018. The nursery was planted in augmented design. The resistant and susceptible checks were repeated and planted after each 10 tested entries. The unit plot size was 2 rows of one meter length for each genotype planted continuously with 25cm row to row spacing. The seed rate was 100 kg/ha. The recommended fertilizer dose of 23:30:0 N:P2O5:K2O kg/ha was applied. The double digit scale (00 to 99) was used to measure overall foliar infection on the whole plant during flowering, soft dough and hard dough stages. Other agronomic practices were followed as per recommendation. Genotypes B86019-1K-3K-0K3, ACC 2087, ACC 2441, ACC GHv-06816, ACC 1597, ACC 1612, ACC 2059 and ACC 2032 were resistant against spot blotch disease. Similarly, 32 barley genotypes were moderately resistant and rest of the tested genotypes were susceptible to the disease. The selected resistant barley genotypes can be used in crossing program and/or promoted for further testing to develop spot blotch resistant varieties for inner Tarai region of Nepal

Understanding metabolic flux behaviour in whole-cell model output

Whole-cell modelling is a newly expanding field that has many applications in lab experiment design and predictive drug testing. Although whole-cell model output contains a wealth of information, it is complex and high dimensional and thus hard to interpret. Here, we present an analysis pipeline that combines machine learning, dimensionality reduction, and network analysis to interpret and visualise metabolic reaction fluxes from a set of single gene knockouts simulated in the Mycoplasma genitalium whole-cell model. We found that the reaction behaviours show trends that correlate with phenotypic classes of the simulation output, highlighting particular cellular subsystems that malfunction after gene knockouts. From a graphical representation of the metabolic network, we saw that there is a set of reactions that can be used as markers of a phenotypic class, showing their importance within the network. Our analysis pipeline can support the understanding of the complexity of in silico cells without detailed knowledge of the constituent parts, which can help to understand the effects of gene knockouts and, as whole-cell models become more widely built and used, aid genome design

Study Of Hard Coral (Scleractinia) Recruitment In The Molas Waters, Manado City

Coral recruitment is the entry of new coral individuals into coral reef populations due to reproduction or migration. Juvenile coral is the result of metamorphosis and growth of coral planula measuring 5 cm and attached to certain substrates. There are two types of substrates in the waters, stable substrates and unstable substrates such as rubble. The purpose of this study was to study hard coral recruitment, both in terms of density, the composition of juvenile coral colony types, size, and the type of substrate occupied by juvenile corals. This research was conducted in August 2021 in the waters of Molas, Bunaken District, Manado City. The method used for site selection is the purposive sampling method and data collection using a sampling method with quadrant transects. The results showed that the density of coral recruitment at the study site was 8,43 colonies/m2. The percentage of juvenile coral attachment on stable substrates is 90% and 10% on unstable substrates. The average size of juvenile corals at the study site was 2.4cm. The composition of the juvenile coral genus found at the research site were corals of the genus Acropora, Anacropora, Coeloseris, Cycloseris, Cyphastrea, Echinopora, Favites, Fungia, Galaxea, Goniastrea, Isopora, Leptastrea, Leptoseris, Lobophyllia, Merulina, Montastrea, Montipora, Oulophyllia, Pachyseris, Pavona, Platygyra, Plasiastrea, Pocillopora, Porites, Psammocora, Scolymia, Seriatopora, Symphyllia, and Trachyphyllia. Of the entire genus, corals of the genus Porites were dominated by the number of juveniles as many as 36 colonies.Keywords: Molas, coral, recruitment, ScleractiniaAbstrakRekrutmen karang adalah masuknya individu karang baru pada populasi terumbu karang dikarenakan reproduksi ataupun migrasi.  Juvenil karang merupakan bentuk hasil metamorphosis dan pertumbuhan planula karang yang berukuran ≤ 5 cm dan menempel pada substrat tertentu.  Terdapat dua tipe substrat di perairan, substrat yang stabil dan substrat yang tidak stabil seperti pecahan karang (rubble).  Tujuan penelitian ini adalah untuk mempelajari tentang rekruitmen karang keras, baik dari densitas, komposisi jenis koloni juvenil karang, ukuran, hingga tipe substrat yang ditempati oleh juvenil karang.  Penelitian ini dilakukan pada bulan Agustus 2021 di perairan Molas Kecamatan Bunaken Kota Manado.  Metode yang digunakan untuk pemilihan lokasi adalah metode purposive sampling dan pengumpulan data menggunakan metode sampling dengan transek kuadran.  Hasil penelitian menunjukan bahwa densitas rekruitmen karang pada lokasi penelitian sebesar 8,43 koloni/m2.  Persentase penempelan juvenil karang pada substrat stabil sebesar 90% dan pada substrat yang tidak stabil sebesar 10%.  Ukuran rata-rata dari juvenil karang pada lokasi penelitian adalah 2,4cm.  Komposisi genus juvenil karang yang ditemukan pada lokasi penelitian adalah karang genus Acropora, Anacropora, Coeloseris, Cycloseris, Cyphastrea, Echinopora, Favites, Fungia, Galaxea, Goniastrea, Isopora, Leptastrea, Leptoseris, Lobophyllia, Merulina, Montastrea, Montipora, Oulophyllia, Pachyseris, Pavona, Platygyra, Plasiastrea, Pocillopora, Porites, Psammocora, Scolymia, Seriatopora, Symphyllia, dan Trachyphyllia.  Dari keseluruhan genus, didominasi oleh karang genus Porites dengan jumlah juvenil sebanyak 36 koloni.Kata kunci: Molas, karang, rekrutmen, scleractini

Nature and chemical abundances of a sample of Lyman-α emitter objects at high redshift

We built a grid of photoionization models and compiled already available observational emission line intensities (1000 53. Values for C/O abundance ratio derived for the LAEs seem to be consistent with those derived for local star forming objects with similar metallicities, while an overabundance of N/O was found for most of the LAEs.Instituto de Astrofísica de La Plat

Nature and chemical abundances of a sample of Lyman-α emitter objects at high redshift

We built a grid of photoionization models and compiled already available observational emission line intensities (1000 53. Values for C/O abundance ratio derived for the LAEs seem to be consistent with those derived for local star forming objects with similar metallicities, while an overabundance of N/O was found for most of the LAEs.Instituto de Astrofísica de La Plat

New metallicity calibration for Seyfert 2 galaxies based on the N2O2 index

We derive a new relation between the metallicity of Seyfert 2 active galactic nuclei (AGNs) and the intensity of the narrow emission-lines ratio N2O2 = log([N II] λ6584/[O II] λ3727). The calibration of this relation was performed by determining the metallicity (Z) of a sample of 58 AGNs through a diagram containing the observational data and the results of a grid of photoionization models obtained with the CLOUDY code. We find the new Z/Z⊙–N2O2 relation using the obtained metallicity values and the corresponding observational emission-line intensities for each object of the sample. Estimations derived through the use of this new calibration indicate that the narrow-line regions of Seyfert 2 galaxies exhibit a large range of metallicities (0.3 ≲ Z/Z⊙ ≲ 2.0), with a median value Z ≈ Z⊙. Regarding the possible existence of correlations between the luminosity L(Hβ), the electron density and the colour excess E(B − V) with the metallicity in this kind of objects, we do not find correlations between them.Instituto de Astrofísica de La PlataFacultad de Ciencias Astronómicas y Geofísica

Incorporating ‘recruitment’ in matrix projection models : estimation, parameters, and the influence of model structure

Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Journal of Ornithology 152, Suppl.2 (2012):585-595, doi:10.1007/s10336-010-0573-1.Advances in the estimation of population parameters using encounter data from marked individuals have made it possible to include estimates of the probability of recruitment in population projection models. However, the projected growth rate of the population, and the sensitivity of projected growth to changes in recruitment, can vary significantly depending upon both the structural form of the model and how recruitment is parameterized. We show that the common practices of (1) collapsing some age classes into a single, terminal ‘aggregated’ age-class, and (2) parameterizing recruitment using the proportion of recruited individuals (breeders) in a given age-class may confound analysis of age-based (Leslie) matrix projection models in some instances, relative to state-based projection models where recruited and pre-recruited individuals are treated as separate states. Failing to account for these differences can lead to misinterpretation of the relative role of recruitment in the dynamics of an age-structured population.We show that such problems can be avoided, either by structural changes to the terminal aggregated age-class in age-based models, or by using using a state-based model instead. Since all the metrics of general interest from a classical age-based matrix models are readily derived from a state-based model equivalent, this suggests there may be little reason to use the classical age-based approach in situations where recruitment is a parameter of interest

Podoconiosis in East and West Gojam Zones, Northern Ethiopia

Background: Podoconiosis is a neglected tropical disease (NTD) that is prevalent in red clay soil-covered highlands of tropical Africa, Central and South America, and northern India. It is estimated that up to one million cases exist in Ethiopia. This study aimed to estimate the prevalence of podoconiosis in East and West Gojam Zones of Amhara Region in northern Ethiopia. Methodology/Principal Findings: A cross-sectional household survey was conducted in Debre Eliyas and Dembecha woredas (districts) in East and West Gojam Zones, respectively. The survey covered all 17,553 households in 20 kebeles (administrative subunits) randomly selected from the two woredas. A detailed structured interview was conducted on 1,704 cases of podoconiosis identified in the survey. Results: The prevalence of podoconiosis in the population aged 15 years and above was found to be 3.3% (95% CI, 3.2% to 3.6%). 87% of cases were in the economically active age group (15–64 years). On average, patients sought treatment five years after the start of the leg swelling. Most subjects had second (42.7%) or third (36.1%) clinical stage disease, 97.9% had mossy lesions, and 53% had open wounds. On average, patients had five episodes of acute adenolymphangitis (ALA) per year and spent a total of 90 days per year with ALA. The median age of first use of shoes and socks were 22 and 23 years, respectively. More men than women owned more than one pair of shoes (61.1% vs. 50.5%; x2 = 11.6 p = 0.001). At the time of interview, 23.6% of the respondents were barefoot, of whom about two-thirds were women. Conclusions: This study showed high prevalence of podoconiosis and associated morbidities such as ALA, mossy lesions and open wounds in northern Ethiopia. Predominance of cases at early clinical stage of podoconiosis indicates the potential for reversing the swelling and calls for disease prevention interventions