Where is Cuba going? A prospective analysis

Traballo de Fin de Grao en Ciencia Política e da Administración. Curso 2015-2016El estudio tiene como objetivo conocer en profundidad la realidad cubana. Establecer para ello diferentes aproximaciones teóricas y analíticas que nos permitan entender su evolución y rasgos principales. Presentar una explicación coherente y bien fundada acerca de los cambios que se están produciendo. Ver hasta qué punto estos cambios pueden transformar el orden económico, político e incluso social de la Isla. Identificar los factores críticos que conducen estos cambios. Situarlos en el contexto en el que tienen lugar. Aplicar la técnica de construcción de escenarios y explorar sus ventajas así como identificar sus límites e inconvenientes. Construir imágenes de futuro a partir de la evolución que lógicamente se desprenda de estos factores

Terapia ocupacional en la enfermedad de Alzheimer

Documento con distintas estrategias de intervención en personas con Enfermedad de Alzheimer, se especifica en dicho documento una pequeña introducción, los objetivos de la intervención, un esquema de tratamiento a nivel físico y a nivel cognitivo y por último distintas terapias no farmacológicas de intervención en la patología

Principios básicos de la movilización de residentes en centros geriátricos

Documento con distintas estrategias de intervención en personas dependientes, donde se especifican también la forma correcta de realizar bien una movilización o una transferencia siguiendo una buena ergonomía postural y así evitar trastornos musculo-esqueléticos

Sequence‐Selective DNA Recognition with Peptide–Bisbenzamidine Conjugates

This is the peer reviewed version of the following article: Sánchez, M. I., Vázquez, O. , Vázquez, M. E. and Mascareñas, J. L. (2013), Sequence‐Selective DNA Recognition with Peptide–Bisbenzamidine Conjugates. Chem. Eur. J., 19: 9923-9929, which has been published in final form at https://doi.org/10.1002/chem.201300519. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived VersionsTranscription factors (TFs) are specialized proteins that play a key role in the regulation of genetic expression. Their mechanism of action involves the interaction with specific DNA sequences, which usually takes place through specialized domains of the protein. However, achieving an efficient binding usually requires the presence of the full protein. This is the case for bZIP and zinc finger TF families, which cannot interact with their target sites when the DNA binding fragments are presented as isolated monomers. Herein it is demonstrated that the DNA binding of these monomeric peptides can be restored when conjugated to aza‐bisbenzamidines, which are readily accessible molecules that interact with A/T‐rich sites by insertion into their minor groove. Importantly, the fluorogenic properties of the aza‐benzamidine unit provide details of the DNA interaction that are eluded in electrophoresis mobility shift assays (EMSA). The hybrids based on the GCN4 bZIP protein preferentially bind to composite sequences containing tandem bisbenzamidine–GCN4 binding sites (TCAT⋅AAATT). Fluorescence reverse titrations show an interesting multiphasic profile consistent with the formation of competitive nonspecific complexes at low DNA/peptide ratios. On the other hand, the conjugate with the DNA binding domain of the zinc finger protein GAGA binds with high affinity (KD≈12 nM) and specificity to a composite AATTT⋅GAGA sequence containing both the bisbenzamidine and the TF consensus binding sitesWe thank the financial support provided by the Spanish grants Consolider Ingenio 2010 (SAF2010‐20822‐C02, CTQ2009‐14431/BQU, CTQ2012‐31341, CSD2007‐00006), the Xunta de Galicia (GRC2010/12, INCITE09 209 084PR, PGIDIT08CSA‐047209PR). M.I.S. thanks the Ministerio de Educación, Cultura y Deporte for his PhD fellowshipS

The ββα fold of zinc finger proteins as a “natural” protecting group. Chemoselective synthesis of a DNA-binding zinc finger derivative

We report the selective modification of cysteine residues engineered in peptides that have two additional cysteine residues as part of a Cys2His2 zinc finger motif. The chemoselective modification is achieved, thanks to the protecting effect exerted by the zinc cation upon coordination with the native cysteines and histidines of the zinc-finger fold. The strategy allows a straightforward synthesis of DNA binding zinc finger constructsWe acknowledge the support from the Spanish grants SAF2010-20822-C02, CTQ2009-14431/BQU, CSD2007-00006, the Xunta de Galicia GRC2010/12, GRC2013-041 and INCITE09 209 084PR. J. R. thanks the Fundación Gil Dávila and the Xunta de Galicia and J. M. the Spanish MCINN for their PhD fellowshipsS

Straightforward access to bisbenzamidine DNA binders and their use as versatile adaptors for DNA-promoted processes

Bisbenzamidines are an important family of minor groove DNA-binding agents. We present a one-step synthesis of aromatic aza-bisbenzamidines that allows straightforward and versatile access to a large number of these molecules. One of them, the azide-aza-bisbenzamidine 13, can be readily modified via click-chemistry with a variety of functionalities that can, therefore, be delivered to the vicinity of an A/T-rich DNA minor groove. This strategy, therefore, provides a simple means for triggering site selective, DNA-promoted biochemical and physicochemical processesS

Spatiotemporal parameters of gait during treadmill and overground walking in Parkinson's disease

[Abstract] Despite the increasing number of studies that have examined the therapeutic effect of treadmill training programs in Parkinson's disease (PD), there has been little research to evaluate the modifications of gait induced by treadmill walking. We investigated spatiotemporal differences between treadmill and overground walking in patients with PD. PD patients significantly increased their step length and step height; and reduced their cadence, step width and step width variability on the treadmill in comparison with walking overground. PD patients are able to attenuate their short shuffling steps when walking on a treadmill.Ministerio de Ciencia e Innovación; DEP2011-2246

The role of moisture transport for precipitation in the inter-annual and inter-daily fluctuations of the Arctic sea ice extension

By considering the moisture transport for precipitation (MTP) for a target region to be the moisture that arrives in this region from its major moisture sources and which then results in precipitation in that region, we explore (i) whether the MTP from the main moisture sources for the Arctic region is linked with inter-annual fluctuations in the extent of Arctic sea ice superimposed on its decline and (ii) the role of extreme MTP events in the inter-daily change in the Arctic sea ice extent (SIE) when extreme MTP simultaneously arrives from the four main moisture regions that supply it. The results suggest (1) that ice melting at the scale of inter-annual fluctuations against the trend is favoured by an increase in moisture transport in summer, autumn, and winter and a decrease in spring and, (2) on a daily basis, extreme humidity transport increases the formation of ice in winter and decreases it in spring, summer, and autumn; in these three seasons extreme humidity transport therefore contributes to Arctic sea ice melting. These patterns differ sharply from that linked to the decline on a long-range scale, especially in summer when the opposite trend applies, as ice melt is favoured by a decrease in moisture transport for this season at this scale.Ministerio de Ciencia e Innovación | Ref. CGL2015-65141-

Atmospheric moisture sources associated with extreme precipitation during the peak precipitation month

Understanding moisture transport is crucial for understanding continental precipitation and extreme precipitation events, which are expected to become substantially more frequent under global warming conditions. In this work, the influence of major global moisture sources on extreme continental precipitation during the peak precipitation month is estimated and compared with climatological patterns. The results show a general change in the pattern of contribution of the primary source of precipitation (the source with the highest contribution) during extreme precipitation events. Most primary sources show a general reduction in their area of influence and a notable reduction in their contribution to the total precipitation.Agencia Estatal de Investigación | Ref. RTI2018‐095772‐B‐I0OXunta de Galicia | Ref. ED481B 2018/062Xunta de Galicia | Ref. ED431C 2017/64Fundação para a Ciência e a Tecnologi