Gauge Invariant Pauli-Villars Regularization of Chiral Fermions

We extend the idea of the generalized Pauli-Villars regularization of Frolov and Slavnov and analyze the general structure of the regularization scheme. The gauge anomaly-free condition emerges in a simple way in the scheme, and, under the standard prescription for the momentum assignment, the Pauli-Villars Lagrangian provides a gauge invariant regularization of chiral fermions in arbitrary anomaly-free representations. The vacuum polarization tensor is transverse, and the fermion number and the conformal anomalies have gauge invariant forms. We also point out that the real representation can be treated in a straightforward manner and the covariant regularization scheme is directly implemented.Comment: 33 pages, PHYZZX. The final version to appear in Prog. Theor. Phy

Invariant Regularization of Supersymmetric Chiral Gauge Theory

We formulate a manifestly supersymmetric gauge covariant regularization of supersymmetric chiral gauge theories. In our scheme, the effective action in the superfield background field method above one-loop is always supersymmetric and gauge invariant. The gauge anomaly has a covariant form and can emerge only in one-loop diagrams with all the external lines being the background gauge superfield. We also present several illustrative applications in the one-loop approximation: the self-energy part of the chiral multiplet and of the gauge multiplet; the super-chiral anomaly and the superconformal anomaly; as the corresponding anomalous commutators, the Konishi anomaly and an anomalous supersymmetric transformation law of the supercurrent (the ``central extension'' of N=1 supersymmetry algebra) and of the R-current.Comment: 43 pages, PHYZZX. Final version to appear in Prog. Theor. Phy

Characterization of some enzymatic properties of recombinant α-glucosidase III from the Thai honeybee, Apis cerana indica Fabricus

Recombinant α-glucosidase III (rHBGase III) from Apis cerana indica Fabricus (rAciHBGase III) was expressed in the yeast Pichia pastoris GS115, enriched and characterized. The full length cDNA of AciHbgase III (ƒî1.8 kb) was amplified by RT-PCR, cloned into the pPICZαA expression vector and used to transform P. pastoris GS115. The maximum secreted expression level of rAciHBGase III [as an N terminal (His)6 tagged chimera] was found 144 h after induction by 1% (v/v) methanol. Enrichment of the enzyme using histrap affinity purification revealed a single active glucosidase band with a molecular mass of ~68 kDa. The optimal pH and temperature for glucosidase activity of the enriched rAciHBGase III were pH 5.0 and 37¢XC, respectively, whilst the enzyme showed a good pH stability between pH 5.0 to 7.5, but not below pH 5.0, and a poor thermotolerance with < 10% and 0% residual activity at 40 and >50¢XC, respectively. The rAciHBGase showed a relatively high substrate specificity for maltose (Km of 4.5 mM) and p-nitrophenyl α-D-glucoside (Km of 4.4 mM) compared to other reported HBGase enzymes.Key words: α-Glucosidase, Apis cerana indica, expression, kinetics, recombinant enzyme

Mate-guarding behavior enhances male reproductive success via familiarization with mating partners in medaka fish

[Background] Male-male competition and female mating preference are major mechanisms of sexual selection, which influences individual fitness. How male-male competition affects female preference, however, remains poorly understood. Under laboratory conditions, medaka (Oryzias latipes) males compete to position themselves between a rival male and the female (mate-guarding) in triadic relationships (male, male, and female). In addition, females prefer to mate with visually familiar males. In the present study, to examine whether mate-guarding affects female preference via visual familiarization, we established a novel behavioral test to simultaneously quantify visual familiarization of focal males with females and mate-guarding against rival males. In addition, we investigated the effect of familiarization on male reproductive success in triadic relationships. [Results] Three fish (female, male, male) were placed separately in a transparent three-chamber tank, which allowed the male in the center (near male) to maintain closer proximity to the female than the other male (far male). Placement of the wild-type male in the center blocked visual familiarization of the far male by the female via mate-guarding. In contrast, placement of an arginine-vasotocin receptor mutant male, which exhibits mate-guarding deficits, in the center, allowing for maintaining close proximity to the female, did not block familiarization of the far male by the female. We also demonstrated that the reproductive success of males was significantly decreased by depriving females visual familiarization with the males. [Conclusions] Our findings indicated that, at least in triadic relationships, dominance in mate-guarding, not simply close proximity, allows males to gain familiarity with the female over their rivals, which may enhance female preference for the dominant male. These findings focusing on the triadic relationships of medaka may contribute to our understanding of the adaptive significance of persistent mate-guarding, as well as female preference for familiar mates

Mate-guarding behavior enhances male reproductive success via familiarization with mating partners in medaka fish

Background: Male-male competition and female mating preference are major mechanisms of sexual selection, which influences individual fitness. How male-male competition affects female preference, however, remains poorly understood. Under laboratory conditions, medaka (Oryzias latipes) males compete to position themselves between a rival male and the female (mate-guarding) in triadic relationships (male, male, and female). In addition, females prefer to mate with visually familiar males. In the present study, to examine whether mate-guarding affects female preference via visual familiarization, we established a novel behavioral test to simultaneously quantify visual familiarization of focal males with females and mate-guarding against rival males. In addition, we investigated the effect of familiarization on male reproductive success in triadic relationships. Results: Three fish (female, male, male) were placed separately in a transparent three-chamber tank, which allowed the male in the center (near male) to maintain closer proximity to the female than the other male (far male). Placement of the wild-type male in the center blocked visual familiarization of the far male by the female via mate-guarding. In contrast, placement of an arginine-vasotocin receptor mutant male, which exhibits mate-guarding deficits, in the center, allowing for maintaining close proximity to the female, did not block familiarization of the far male by the female. We also demonstrated that the reproductive success of males was significantly decreased by depriving females visual familiarization with the males. Conclusions: Our findings indicated that, at least in triadic relationships, dominance in mate-guarding, not simply close proximity, allows males to gain familiarity with the female over their rivals, which may enhance female preference for the dominant male. These findings focusing on the triadic relationships of medaka may contribute to our understanding of the adaptive significance of persistent mate-guarding, as well as female preference for familiar mates

Transient and permanent gene transfer into the brain of the teleost fish medaka (Oryzias latipes) using human adenovirus and the Cre-loxP system

AbstractIn this study, we demonstrated that human type-5 adenovirus infected the brain of the teleost fish, medaka (Oryzias latipes), in vivo. Injection of adenoviral vector into the mesencephalic ventricle of medaka larvae induced the expression of reporter genes in some parts of the telencephalon, the periventricular area of the mesencephalon and diencephalon, and the cerebellum. Additionally, the Cre-loxP system works in medaka brains using transgenic medaka carrying a vector containing DsRed2, flanked by loxP sites under control of the β-actin promoter and downstream promoterless enhanced green fluorescent protein (EGFP). We demonstrated that the presence of green fluorescence depended on injection of adenoviral vector expressing the Cre gene and confirmed that EGFP mRNA was transcribed in the virus-injected larvae

Remark on the Consistent Gauge Anomaly in Supersymmetric Theories

We present a direct field theoretical calculation of the consistent gauge anomaly in the superfield formalism, on the basis of a definition of the effective action through the covariant gauge current. The scheme is conceptually and technically simple and the gauge covariance in intermediate steps reduces calculational labors considerably. The resultant superfield anomaly, being proportional to the anomaly d^{abc}=\tr T^a\{T^b,T^c\}, is minimal without supplementing any counterterms. Our anomaly coincides with the anomaly obtained by Marinkovi\'c as the solution of the Wess-Zumino consistency condition.Comment: uses PHYZZX, 17 pages, the result of SUSY anomaly in the Wess-Zumino gauge adde

Manifestly Gauge Covariant Treatment of Lattice Chiral Fermions. II

We propose a new formulation of chiral fermions on a lattice, on the basis of a lattice extension of the covariant regularization scheme in continuum field theory. The species doublers do not emerge. The real part of the effective action is just one half of that of Dirac-Wilson fermion and is always gauge invariant even with a finite lattice spacing. The gauge invariance of the imaginary part, on the other hand, sets a severe constraint which is a lattice analogue of the gauge anomaly free condition. For real gauge representations, the imaginary part identically vanishes and the gauge invariance becomes exact.Comment: 15 pages, PHYZZX. The title is changed. The final version to appear in Phys. Rev.

More about the axial anomaly on the lattice

We study the axial anomaly defined on a finite-size lattice by using a Dirac operator which obeys the Ginsparg-Wilson relation. When the gauge group is U(1), we show that the basic structure of axial anomaly on the infinite lattice, which can be deduced by a cohomological analysis, persists even on (sufficiently large) finite-size lattices. For non-abelian gauge groups, we propose a conjecture on a possible form of axial anomaly on the infinite lattice, which holds to all orders in perturbation theory. With this conjecture, we show that a structure of the axial anomaly on finite-size lattices is again basically identical to that on the infinite lattice. Our analysis with the Ginsparg-Wilson Dirac operator indicates that, in appropriate frameworks, the basic structure of axial anomaly is quite robust and it persists even in a system with finite ultraviolet and infrared cutoffs.Comment: 12 pages, uses JHEP.cls and amsfonts.sty, the final version to appear in Nucl. Phys.