30S Beam Development and X-ray Bursts

Over the past three years, we have worked on developing a well-characterized 30S radioactive beam to be used in a future experiment aiming to directly measure the 30S(alpha,p) stellar reaction rate within the Gamow window of Type I X-ray bursts. The importance of the 30S(alpha,p) reaction to X-ray bursts is discussed. Given the astrophysical motivation, the successful results of and challenges involved in the production of a low-energy 30S beam are detailed. Finally, an overview of our future plans regarding this on-going project are presented.Comment: 7 pages, 2 figures, 5th European Summer School on Experimental Nuclear Astrophysics, Santa Tecla, Sicily, September 200

Two-Dimensional Molecular Patterning by Surface-Enhanced Zn-Porphyrin Coordination

In this contribution, we show how zinc-5,10,15,20-meso-tetradodecylporphyrins (Zn-TDPs) self-assemble into stable organized arrays on the surface of graphite, thus positioning their metal center at regular distances from each other, creating a molecular pattern, while retaining the possibility to coordinate additional ligands. We also demonstrate that Zn-TDPs coordinated to 3-nitropyridine display a higher tendency to be adsorbed at the surface of highly oriented pyrolytic graphite (HOPG) than noncoordinated ones. In order to investigate the two-dimensional (2D) self-assembly of coordinated Zn-TDPs, solutions with different relative concentrations of 3-nitropyridine and Zn-TDP were prepared and deposited on the surface of HOPG. STM measurements at the liquid-solid interface reveal that the ratio of coordinated Zn-TDPs over noncoordinated Zn-TDPs is higher at the n-tetradecane/HOPG interface than in n-tetradecane solution. This enhanced binding of the axial ligand at the liquid/solid interface is likely related to the fact that physisorbed Zn-TDPs are better binding sites for nitropyridines.

DEVELOPMENT OF "Luftain -Tg" AS A MICROISOLATION CAGE SYSTEM

Developed "Luftain Tg" to include cage and luck for the breeding, nowadays an experiment increases as exchanging it digest gene set, and breeding, experiment of Transgenic mouse (following Tg mouse) increases. Doesn't exit the front of luck to outside as giving and doing a mind directly to cage inside, as a ceremony as hanging bathtub type cage-we controlled with a curtain and developed a system to defend from an expansion of cage inside and outside contamination. Again, for flowing of these air computer simulation even though were able to confirm it.Tgマウスの飼育に適し､かつ､飼育作業性の良いラックの開発を行った｡バスタブ型ケージを吊り式とし､天井／吊りレール／背面側塞ぎ板により上方3方を閉鎖しケージからの排気出口は上前面のケージ鍔上のみとした｡ケージ-の上後方からの直接給気とケージからの排気の流れは架台前面をエアカーテンで制御し､架台後方より排気することによりケージ内外の汚染の伝播を防止する機構になっており､コンピューター･シミュレーション及び気流可視化試験によりその効果が確認できた

Hypertrophic pyloric stenosis: tips and tricks for ultrasound diagnosis

We describe a systematic approach to the ultrasound (US) examination of the antropyloric region in children. US is the modality of choice for the diagnosis of hypertrophic pyloric stenosis (HPS). The imaging features of the normal pylorus and the diagnostic findings in HPS are reviewed and illustrated in this pictorial essay. Common difficulties in performing the examination and tips to help overcome them will also be discussed

Effects of Soil Types and Fertilizers on Growth, Yield, and Quality of Edible Amaranthus tricolor lines in Okinawa, Japan

Soil types and fertilizer regimes were evaluated on growth, yield, and quality of Amaranthus tricolor lines, IB (India Bengal), TW (Taiwan), BB (Bangladesh B), and BC (Bangladesh C) in developing management practices in Okinawa. Growth and yield of all amaranth lines were higher in gray soil (pH 8.4) than in dark red soil (pH 6.6) and red soil (pH 5.4). The combined NPK fertilizer resulted in highest growth parameters and yield of amaranths in all soils. Nitrogen fertilizer alone did not affect growth parameters and yield of amaranths in dark red and red soils. Growth parameters and yield increased similarly with the 30, 40, and 50 g m−2 of NPK fertilizer in BB line, and with the 20, 30, 40, and 50 g m−2 in BC line. Agronomic efficiency of NPK fertilizer at 50 g m−2 was not prominent on the amaranths, compared to the fertilizer at 40 g m−2. Amaranth lines had higher Na in dark red and red soils, while K and Mg in gray soil, Ca in gray and red soils, and Fe in dark red soil. The NPK fertilizer resulted in higher Na, Ca, Mg, and P in BB line in glasshouse. These minerals in BB line were not clearly affected, but in BC line were lower with NPK fertilizer at 20–50 g m−2 in field. These studies indicate that gray soil is best for amaranth cultivation and combined NPK fertilizer at 20–40 g m−2 is effective in gray soil in Okinawa for higher yield and minerals of amaranth

Bidirectional autoregulatory mechanism of metastasis-associated protein 1-alternative reading frame pathway in oncogenesis

Although metastasis-associated protein 1 (MTA1), a component of the nucleosome remodeling and histone deacetylation complex, is widely up-regulated in human cancers and correlates with tumor metastasis, its regulatory mechanism and related signaling pathways remain unknown. Here, we report a previously unrecognized bidirectional autoregulatory loop between MTA1 and tumor suppressor alternative reading frame (ARF). MTA1 transactivates ARF transcription by recruiting the transcription factor c-Jun onto the ARF promoter in a p53-independent manner. ARF, in turn, negatively regulates MTA1 expression independently of p53 and c-Myc. In this context, ARF interacts with transcription factor specificity protein 1 (SP1) and promotes its proteasomal degradation by enhancing its interaction with proteasome subunit regulatory particle ATPase 6, thereby abrogating the ability of SP1 to stimulate MTA1 transcription. ARF also physically associates with MTA1 and affects its protein stability. Thus, MTA1-mediated activation of ARF and ARF-mediated functional inhibition of MTA1 represent a p53-independent bidirectional autoregulatory mechanism in which these two opposites act in concert to regulate cell homeostasis and oncogenesis, depending on the cellular context and the environment

IL-6 is constitutively expressed during lung morphogenesis and enhances fetal lung explant branching

Previous studies have shown that chorioamnionitis, with increased IL-6, promotes fetal lung maturation and decreases the incidence of respiratory distress syndrome in premature neonates. However, the expression pattern and the effects of IL-6 on fetal lung growth mechanisms remain unknown. IL-6 expression was assessed by in situ hybridization and by real-time PCR between 14.5 and 21.5 d postconception. Normal and nitrofen-induced hypoplastic lung explants were cultured with increasing IL-6 doses or IL-6 neutralizing antibodies. Branching, cellular proliferation (Ki-67) and MAPK phosphorylation in fetal lung explants were analyzed. Pulmonary primitive epithelium expressed IL-6 constitutively throughout all gestational ages, displaying highest levels during earliest stages. In normal and hypoplastic lung explants, IL-6 neutralizing antibodies significantly reduced, whereas IL-6 supplementation induced a biphasic effect (lower doses increased, while the highest dose did not accomplish additional effect) on branching and cellular proliferation. IL-6 enhanced p38-MAPK phosphorylation without changing MEK1/2 and JNK pathways. The present study suggests a physiological role for IL-6 on pulmonary branching mechanisms most likely involving p38-MAPK intracellular signalling pathway

The novel estrogen-induced gene EIG121 regulates autophagy and promotes cell survival under stress

We previously identified a novel estrogen-induced gene, EIG121, as being differentially regulated in endometrioid and nonendometrioid endometrial carcinoma. The function of EIG121 was unknown. Using a tetracycline-inducible system, we found that overexpression of EIG121, but not of LacZ, caused a profound suppression of cell growth. Subcellular fractionation and immunofluroscent labeling indicated that EIG121 was a transmembrane protein localized in the plasma membrane-late endosome–lysosome compartments. Deletion of the putative transmembrane domain abolished the membrane association. In cells overexpressing EIG121, cytoplasmic vacuoles accumulated after EIG121 induction, and the autophagosome marker LC3 translocated into punctuate, dot-like structures. Electron microscopy revealed that in cells overexpressing EIG121, autophagosomes were markedly increased. Overexpression of EIG121 also increased the cells containing acidic vesicles and induced lysosomal degradation of long-lived proteins. In MCF-7 cells, both EIG121 and LC3 were rapidly degraded by a lysosomal mechanism after starvation. Knockdown of EIG121 blocked starvation-induced LC3 degradation. By itself, knockdown of EIG121 did not affect cell survival. When combined with starvation or cytotoxic agents, EIG121 knockdown greatly increased apoptosis. Our results suggest that EIG121 is associated with the endosome–lysosome compartments and may have an important role in autophagy. Under unfavorable conditions such as starvation and exposure to cytotoxic agents, EIG121 may protect cells from cell death by upregulating the autophagy pathway

Brevianes Revisited

Breviones are a new family of secondary metabolites that were originally isolated from the New Zealand endemic fungus Penicillium brevicompactum var. Dierckx. These compounds are generally characterized by a new carbon skeleton, known as breviane, which that has three possible structural variations, such as breviane, abeo-breviane, and abeo-norbreviane. Brevianes present a basic diterpenic tricyclic core that is mevalonic in origin and is similar to that of perhydrophenanthrene. The core bears four methyl groups at positions C4, C8, C10, and C13 and has defined stereochemistry at positions C5, C8, C9, C10, and C14. The C1'-C7' side chain has been proposed to have a polyketide biosynthetic origin and is joined to the diterpenic moiety through carbons C2'-C15'. The cyclization and lactonization of this part of the molecule leads to the characteristic breviane spiranic ring fused to the α-pyrone

Functional Consequences of the Human Leptin Receptor ( LEPR ) Q223R Transversion

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/93715/1/oby.2008.489.pd