Copper, Nickel and Manganese Residues in Tissues and Organs of Rabbits Dosed with Edible Clay

The study examined the kinetics of Copper (Cu), Nickel (Ni) and Manganese (Mn) in tissues and organs of rabbits treated with edible clay from Enyigba, Ebonyi state Nigeria. Clay samples were obtained from Enyigba mine. Fifty four of the sampled rabbits were grouped into 3: group 1 and group 2 were administered with 2000 mg/kg and 4000 mg/kg of edible clay respectively while group 3 served as control. Concentrations of these metals were detected in the brain, heart, kidney, liver, lungs, skeletal muscle and blood of each of the rabbits using atomic absorption spectroscopy (AAS). One - way ANOVA was used in analyzing the concentrations with significant results (p < 0.05) recorded. The study results showed that while Cu concentrations were predominant in the liver (15.33±1.18 µg/g), kidney (8.25±1.18 µg/g) and heart (9.43±2.36 µg/g) of 2000 mg/kg treated rabbits on day 1 post-treatment, Ni concentrations were the highest among the three elements especially in the blood (221.59±17.05 µg/g) and skeletal muscle (153.41±19.68 µg/g) of 2000 mg/kg treated rabbits on day 1 post-treatment. Concentrations of Mn were mostly detected in the liver (13.99±4.85 µg/g) of 2000 mg/kg treated rabbits on day 1 post-treatment. Most of the concentration values increased in 4000 mg/kg treated rabbits on day 1 post-treatment. Elimination constants as well as half-life eliminations of the metals studied showed that absorbed elements were still present in most organs and tissues of treated rabbits. The study recommends research for possible microbial toxins that may be found in the edible clay studied

Diurnal Temperature Range as Index of Climate Change in Lafia, Nasarawa State, Nigeria

The study focused on determining the Diurnal temperature range trend as an index of climate change in Lafia, Nasarawa State, Nigeria. The study relied on remote sensing data- the ERA5 – Temperature, which is the fifth generation reanalysis from ECMWF. The data for the period of forty (40) years was extracted and used to analyze the annual range, diurnal temperature, trend analysis and daily maximum and minimum temperatures. The results shows a decrease in the trend analysis for the decadal and pentad which means that the difference between the daily maximum temperature and daily minimum temperature is no longer wide. However, the trend analysis carried out for the annual DTR shows a positive trend which shows that the Maximum daily temperature is higher than the daily minimum range in the study area. The study recommended for more intense study of DTR in order to stop over generalizing the index of climate change over a macro area

Effect of Different Sources of Heat on Nutritional Composition of Tilapia Fish (Oreochromis niloticus)

A study was conducted to investigate the effect of traditional smoking methods on fresh tilapia fish (Oreochromis niloticus). For this purpose, proximate, mineral and amino acid compositions of tilapia fish smoked with sawdust, rice bran, melon husk and electric oven (control) heat treatments were determined using standard analytical technique. The results showed that crude protein content (%) ranged between 55.10 to 64.16% with the highest value recorded in sample smoked with rice bran heat treatment while crude fat had the highest value (18.10%) also in rice bran smoked sample. Sodium was found to be more abundant in all the samples (5.75 – 10.10 mg/100g) compared with magnesium (5.45 – 9.15 mg/100g), potassium (4.55 – 6.45 mg/100g) and phosphorus (4.70 – 6.00 mg/100g) while the least abundant mineral was chromium (0.05 – 0.15 mg/100g). The harmful metals such as cadmium and lead were not at detectable range of atomic absorption spectrophotometer (AAS). The amino acid profile revealed that only Oreochromis niloticus fish sample smoked with sawdust enhanced the contents of total amino acid (TAA), total essential amino acid (TEAA) and total sulphur amino acid (TSAA) by 3.77, 3.95 and 7.03%, respectively while melon husk and rice bran heat treatments reduced TAA, TEAA and TSAA. The limiting amino acid (LAA) for all the samples with different heat treatments was Ile except sample smoked with rice bran which has Val as LAA. Generally, all the smoked samples contained nutritionally useful quantities of total essential amino acids. Keywords: Oreochromis niloticus, agricultural wastes, smoke, proximate, amino acids

Physical properties, dietary fibre profile and peroxide value of biscuit produced from wheat-Tigernut flours with avocado paste as fat substitute

This study investigated the effect of replacing fat with avocado paste on the physical properties, dietary fibre profile and peroxide value of wheat-tigernut biscuits. Whole tigernut was processed into flour and blended with wheat flour at three different levels; 10%, 20% and 30% weight basis into two parts. The first part of the composite (T10, T20, and T30) and control sample (100% wheat flour, T00) were baked into biscuit using margarine, while in the second batch (AT10, AT20, and AT30), margarine was replaced with avocado paste (100%). The biscuit samples were analysed for physical properties, dietary fibre profile and peroxide value using standard procedures. Data were subjected to Analysis of variance, and means were separated using Duncan’s Multiple Range Test at p<0.05. Biscuits baked with avocado paste had higher spread ratio (6.98 – 7.19) and weight (17.39 – 17.51 g) than samples baked with margarine. Break strength of control sample was higher (185g) compared to biscuit samples baked with margarine (182.70 – 175.81 g), but lower than biscuits baked with avocado paste (182.70 – 175.81 g). Biscuits baked with avocado had higher dietary fibre profile of 7.49 to 7.84% and 5.72 to 5.82% for insoluble and soluble fibres. Biscuits containing avocado paste had higher peroxide values (1.91 to 2.56 meq O2/kg) than samples containing margarine (1.67 to 2.18 meq O2/kg). Replacing avocado with margarine improved the physical properties and dietary fibre profile of the biscuits with no adverse effect on the peroxide value and could therefore, be exploited as a healthier shortening agent to enrich biscuits

Electrophysiological and behavioral responses of female African rice gall midge, Orseolia oryzivora Harris and Gagné, to host plant volatiles

African rice gall midge, Orseolia oryzivora Harris and Gagné, is a major pest of rice in Africa. Depsite its economic importance, its chemical ecology is not well understood. Here, we assessed behavioral and electrophysiological responses of O. oryzivora to host plant volatiles. In olfactometer bioassays, mated female O. oryzivora were attracted to volatiles emitted from intact rice plants but were repelled by volatiles collected from plants infested by conspecifics. In a choice test, there was a preference for volatiles from uninfested plants over those from infested plants. Coupled gas chromatography-electroantennography analyses of panicle volatiles isolated four electrophysiologically active components: (S)-linalool, 4,8-dimethyl-1,3,7-nonatriene, (E)-caryophyllene, and (R/S)-(E)-nerolidol. A synthetic blend of volatiles at the same concentration and ratio as that from an intact plant was attractive to mated females, whereas a blend based on the ratio of volatiles from an infested plant was repellent. This suggests that O. oryzivora uses olfaction for host plant recognition. The identification of blends of volatiles emitted by plants that can both attract and repel O. oryzivora may aid the development of sustainable control measures

Readmission and death after an acute heart failure event: predictors and outcomes in sub-saharan africa: results from the thesus-hf registry

Aims: Contrary to elderly patients with ischaemic-related acute heart failure (AHF) typically enrolled in North American and European registries, patients enrolled in the sub-Saharan Africa Survey of Heart Failure (THESUS-HF) were middle-aged with AHF due primarily to non-ischaemic causes.We sought to describe factors prognostic of re-admission and death in this developing population. Methods and results: Prognostic models were developed from data collected on 1006 patients enrolled in THESUS-HF, a prospective registry of AHF patients in 12 hospitals in nine sub-Saharan African countries, mostly in Nigeria, Uganda, and South Africa. The main predictors of 60-day re-admission or death in a model excluding the geographic region were a history of malignancy and severe lung disease, admission systolic blood pressure, heart rate and signs of congestion (rales), kidney function (BUN), and echocardiographic ejection fraction. In a model including region, the Southern region had a higher risk. Age and admission sodium levels were not prognostic. Predictors of 180-day mortality included malignancy, severe lung disease, smoking history, systolic blood pressure, heart rate, and symptoms and signs of congestion (orthopnoea, peripheral oedema and rales) at admission, kidney dysfunction (BUN), anaemia, and HIV positivity. Discrimination was low for all models, similar to models for European and North American patients, suggesting that the main factors contributing to adverse outcomes are still unknown. Conclusion: Despite the differences in age and disease characteristics, the main predictors for 6 months mortality and combined 60 days re-admission and death are largely similar in sub-Saharan Africa as in the rest of the world, with some exceptions such as the association of the HIV status with mortality

Hybrid Vigor and Heritability Estimates in Tomato Crosses Involving Solanum lycopersicum × S. pimpinellifolium under Cool Tropical Monsoon Climate

High humidity is a major constraint to increased tomato fruit production in a cool tropical monsoon climate. However, the genetic variation observed in Solanum pimpinellifolium makes it a good gene donor for breeding tomato cultivars capable of thriving under high humidity. The objective of this study was to estimate heterosis, heritability for higher yield, and to assess the adaptability of the genotypes to humid conditions. Genotypes were raised from five morphologically divergent parents, viz., wild parent (W)–“LA2093,” “CLN2498D” (D), “CLN2417H” (H), “Tima” (T), and “UC Dan INDIA” (U). The F1s were generated by biparental mating design using “LA2093” as a common pollen donor that was selfed to produce F2s and backcrossed to both parents to obtain BC1s and BC2s. The trial was laid out in a randomized complete block design with three replicates. Data were collected on selected yield-influencing traits and analyzed. “D × W” and “U × W” hybrids showed significant positive better parent heterosis for fruit weight per plant (30.4% and 35.5%) and total fruit yield (48.6% and 26.9%), respectively. The additive variance was higher than dominance variance for all the traits, including total fruit yield in all hybrids viz., “H × W,” “D × W,” “T × W,” and “U × W.” High narrow sense heritability estimate of ≥60% was observed in “D × W” and “U × W” hybrids for the majority of the floral and fruit traits including total fruit yield. This makes the improvement of “D × W” and “U × W” hybrids by direct selection advantageous. Hence, the adoption of selection for the affected traits in subsequent tomato breeding programs would enhance fruit yield and adaptability to humid environments

Stroke in Africa: Profile, progress, prospects and priorities

Funding text 1 R.O.A. is supported by the UK Royal Society/African Academy of Sciences FLAIR Grants FLR/R1/191813 and FCG/R1/ 201034, and a GCRF Networking Grant from the UK Academy of Medical Sciences. R.O.A., M.O.O., B.O. and F.S.S. are also supported by grants U54HG007479 and U01HG010273 from the US National Institutes of Health (NIH) as part of the H3Africa Consortium. M.O.O., B.O., R.O.A. and F.S.S. are further supported by NIH grant R01NS107900. R.N.K.’s research on elderly survivors of stroke has been supported by the Medical Research Council, RCUK Newcastle Centre for Brain Ageing and Vitality (MRC G0500247), Alzheimer’s Research UK, the Dunhill Medical Trust, UK, and the Newcastle National Institute for Health Research Biomedical Research Centre in Ageing and Age-Related Diseases, Newcastle upon Tyne Hospitals National Health Service Foundation Trust. Funding text 2 funds provided by the Wellcome Trust and the NIH. The NIH-funded SIREN study is exploring the genetic architecture of stroke among Indigenous Africans. More than 4,000 case–control pairs have already been recruited to the study and several publications on stroke phenom-ics and preliminary candidate gene analyses have been generated. The SIREN study has also undertaken the first-ever GWAS to unravel the genetic architecture of stroke in Indigenous Africans and the results are eagerly awaited. Stroke neurobanking resources consisting of blood fractions, extracted DNA, neuroimages and databases of clinical information are also being built in Africa and could facilitate data science-driven trans-omics research (including epigenomics, tran-scriptomics, proteomics and metabolomics) as well as the development of precision medicine products such as Afrocentric risk calculators, polygenic risk scores, biomarkers and drug targets23–25,227,307,308. The SIREN neurobiobank comprises a group of constantly monitored ultra-low-temperature (–86 °C) freezers located in Ibadan, Nigeria, constantly powered –20 °C chest freezers located in Ibadan and other recruitment sites, barcode scanners and printers, a laboratory information management system, a secure multi-terabyte server,Stroke is a leading cause of disability, dementia, and death worldwide. Approximately 70% of deaths from stroke and 87% of stroke-related disabilities occur in low-income and middle-income countries. At the turn of the century, the most common diseases in Africa were communicable diseases, whereas non-communicable diseases, including stroke, were considered rare, particularly in sub-Saharan Africa. However, evidence indicates that today, Africa could have up to 2–3-fold greater rates of stroke incidence and higher stroke prevalence than western Europe and the USA. In Africa, data published within the past decade show that stroke has an annual incidence rate of up to 316 per 100,000, a prevalence of up to 1,460 per 100,000, and a 3-year fatality rate greater than 80%. Moreover, many Africans have a stroke within the fourth to sixth decades of life, with serious implications for the individual, their family, and society. This age profile is particularly important as strokes in younger people tend to result in a greater loss of self-worth and socioeconomic productivity than in older individuals. Emerging insights from research into stroke epidemiology, genetics, prevention, care, and outcomes offer great prospects for tackling the growing burden of stroke on the continent. In this article, we review the unique profile of stroke in Africa and summarize current knowledge on stroke epidemiology, genetics, prevention, acute care, rehabilitation, outcomes, cost of care, and awareness. We also discuss knowledge gaps, emerging priorities, and future directions of stroke medicine for the more than 1 billion people who live in Africa. © 2021, Springer Nature Limited.Newcastle National Institute for Health Research Biomedical Research Centre in Ageing and Age-Related Diseases Newcastle upon Tyne Hospitals National Health Service Foundation Trust RCUK Newcastle Centre for Brain Ageing and Vitality Royal Society/African Academy of Sciences: FCG/R1/ 201034,FLR/R1/191813 National Institutes of Health (NIH): R01NS107900 Wellcome Trust (WT) Medical Research Council (MRC): G0500247 Dunhill Medical Trust (DMT) Academy of Medical Sciences: U01HG010273,U54HG007479 Alzheimer’s Research UK (ARUK

Echocardiographic partition values and prevalence of left ventricular hypertrophy in hypertensive Nigerians

BACKGROUND: Left ventricular hypertrophy (LVH) is a well known independent risk factor for cardiovascular events. It has been shown that combination of left ventricular mass (LVM) and relative wall thickness (RWT) can be used to identify different forms of left ventricular (LV) geometry. Prospective studies have shown that LV geometric patterns have prognostic implications, with the worst prognosis associated with concentric hypertrophy. The methods for the normalization or indexation of LVM have also recently been shown to confer some prognostic value especially in obese population. We sought to determine the prevalence of echocardiographic lLVH using eight different and published cut-off or threshold values in hypertensive subjects seen in a developing country's tertiary centre. METHODS: Echocardiography was performed in four hundred and eighty consecutive hypertensive subjects attending the cardiology clinic of the University college Hospital Ibadan, Nigeria over a two-year period. RESULTS: Complete data was obtained in 457 (95.2%) of the 480 subjects (48.6% women). The prevalence of LVH ranged between 30.9–56.0%. The highest prevalence was when LVM was indexed to the power of 2.7 with a partition value of 49.2 g/ht(2.7 )in men and 46.7 g/ht(2.7 )in women. The lowest prevalence was observed when LVM was indexed to body surface area (BSA) and a partition value of 125 g/m(2 )was used for both sexes. Abnormal LV geometry was present in 61.1%–74.0% of our subjects and commoner in women. CONCLUSION: The prevalence of LVH hypertensive patients is strongly dependent on the cut-off value used to define it. Large-scale prospective study will be needed to determine the prognostic implications of the different LV geometry in native Africans