246 research outputs found

    Music Education in South Africa

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    In this time of extreme national difficulty and uncertainty about the future, South Africa\u27s entire educational system is faltering as political and economic disruption overwhelms the nation\u27s social structure. As South Africa\u27s music educators face the difficulties of changing the traditions of segregation and overemphasis upon Western music forms, they are finding strength in sharing information and their visions for the future of South Africa

    Multiplex Profiling of Cellular Invasion in 3D Cell Culture Models.

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    To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces. Here, we describe a 96-well microplate-based, high-content, three-dimensional cell culture assay capable of assessing invasion dynamics and molecular signatures thereof. On applying our invasion assay, we were able to demonstrate significant effects on the invasion capacity of fibroblast cell lines, as well as primary lung fibroblasts. Administration of epidermal growth factor resulted in a substantial increase of cellular invasion, thus making this technique suitable for high-throughput pharmacological screening of novel compounds regulating invasive and migratory pathways of primary cells. Our assay also correlates cellular invasiveness to molecular events. Thus, we argue of having developed a powerful and versatile toolbox for an extensive profiling of invasive cells in a 96-well format. This will have a major impact on research in disease areas like fibrosis, metastatic cancers, or chronic inflammatory states

    SIRU development. Volume 3: Software description and program documentation

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    The development and initial evaluation of a strapdown inertial reference unit (SIRU) system are discussed. The SIRU configuration is a modular inertial subsystem with hardware and software features that achieve fault tolerant operational capabilities. The SIRU redundant hardware design is formulated about a six gyro and six accelerometer instrument module package. The six axes array provides redundant independent sensing and the symmetry enables the formulation of an optimal software redundant data processing structure with self-contained fault detection and isolation (FDI) capabilities. The basic SIRU software coding system used in the DDP-516 computer is documented

    SIRU utilization. Volume 2: Software description and program documentation

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    A complete description of the additional analysis, development and evaluation provided for the SIRU system as identified in the requirements for the SIRU utilization program is presented. The SIRU configuration is a modular inertial subsystem with hardware and software features that achieve fault tolerant operational capabilities. The SIRU redundant hardware design is formulated about a six gyro and six accelerometer instrument module package. The modules are mounted in this package so that their measurement input axes form a unique symmetrical pattern that corresponds to the array of perpendiculars to the faces of a regular dodecahedron. This six axes array provides redundant independent sensing and the symmetry enables the formulation of an optimal software redundant data processing structure with self-contained fault detection and isolation (FDI) capabilities. Documentation of the additional software and software modifications required to implement the utilization capabilities includes assembly listings and flow chart

    From pyruvate to PEP...the unknown pathway

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    Abstract only availableSoybean is a major crop in the United States. Its survival and relationship with its environment are closely monitored. For example, we are looking at the symbiotic relationship between soybean and the soil bacteria. These bacteria can convert nitrogen in the air into usable nitrogen for the soybean plant. This process occurs when the rhizobium forms a nodule on the soybean root. These nodules are complex, hyperplastic tissue masses derived from cortical cells that transport nitrogen as uerides. The soybean plant then in turn acts as a carbon and energy source for the bacteria. The metabolic pathway for the bacteria's reception and consumption of this carbon is unknown. Our specific focus is to use in gel assays to identify the presence of glyceraldehydes-3-P-dehydrogenase, phosphoglycerate kinase, phosohpoglycerate mutase and enolase, used by the plant to synthesize a reaction to convert glyceraldehydes-3-phosphate into PEP (phosphoenolpyruvate) which will be conducted after my departure from the lab. We first conduct a protein extraction to isolate only the desired material from the soybean plant. We then use a one-dimensional gel enzyme assay to determine whether our four main enzymes are active (we received positive results for all four). Also, a two-dimensional gel enzyme assay to determine whether the enzyme we have found is indeed the one we think we have identified in one dimension (we have found positive results for all except phosphoglycerate mutase). Finally, we use an enzyme assay to determine if there is enzyme activity by measuring the absorbance of a solution containing substrate and introducing the enzyme. We found the presence and activity of all of the crucial enzymes involved in the pathway. We have pretty much concluded that this pathway, formerly thought to be a part of the Alanine Transport model, is more likely to be a part of the plant's metabolism.MU Monsanto Undergraduate Research Fellowshi

    Defining molecular signatures of fibroblast phenotypes in pulmonary fibrosis

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    The grammatical status of the English dative alternation.

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    Thesis. 1976. Ph.D.--Massachusetts Institute of Technology. Dept. of Foreign Literatures and Linguistics.Microfiche copy available in Archives and Humanities.Vita.Bibliography: leaves 286-288.Ph.D

    The Grail theorem prover: Type theory for syntax and semantics

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    As the name suggests, type-logical grammars are a grammar formalism based on logic and type theory. From the prespective of grammar design, type-logical grammars develop the syntactic and semantic aspects of linguistic phenomena hand-in-hand, letting the desired semantics of an expression inform the syntactic type and vice versa. Prototypical examples of the successful application of type-logical grammars to the syntax-semantics interface include coordination, quantifier scope and extraction.This chapter describes the Grail theorem prover, a series of tools for designing and testing grammars in various modern type-logical grammars which functions as a tool . All tools described in this chapter are freely available
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