Prospective assessment of Y-chromosome microdeletions and reproductive outcomes among infertile couples of Japanese and African origin

BACKGROUND: To compare the frequency of Y-chromosome microdeletions in Japanese and African azoospermic and oligozoospermic men and describe embryo characteristics and reproductive outcome following in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). METHODS: Our study was performed prospectively at two centers, a private IVF clinic and a university hospital. Japanese and African (Tanzanian) men with nonobstructive azoospermia (NOA) and oligozoospermia (concentration < 5 × 10(6 )/ml) were evaluated for Y-chromosome microdeletions (n = 162). Of the 47 men with NOA, 26 were Japanese and 21 were Africans. Of the 115 men with oligozoospermia, 87 were Japanese and 28 were Africans. Reproductive outcomes of patients with Y-chromosome microdeletions were then compared with those of 19 IVF+ICSI cycles performed on couples with Y-chromosome intact males/tubal factor infertility which served as a control group. RESULTS: Seven azoospermic and oligozoospermic patients had Y-chromosome deletions; the total number of deletions in the AZFc region was five. There was only one deletion in the AZFa region and one complete deletion involving all three regions (AZFa, b, and c) within AZF. In our study population, microdeletion frequency among Japanese men was 6.2% (95% CI, 4.25% – 14.45%), whereas no deletions were identified in the African group (95% CI, 0.0% – 7.27%). The difference between the two groups was not statistically significant, however. Embryos derived from ICSI utilizing sperm with Y-chromosome microdeletion showed reduced rates of fertilization, blastocyst development, implantation, and pregnancy compared to the Y-chromosome intact group, although these observed differences were not statistically significant. CONCLUSION: The observed frequency of Y-chromosome microdeletion was 6.2% among Japanese azoospermic and oligozoospermic males; no microdeletions were identified among our African study patients. In this population of couples undergoing IVF+ICSI, there was no statistically significant difference in embryo characteristics or pregnancy outcome between patients with Y-chromosome microdeletion and those with an intact Y-chromosome

The response of tropical rainforests to drought : lessons from recent research and future prospects

Key message: we review the recent findings on the influence of drought on tree mortality, growth or ecosystem functioning in tropical rainforests. Drought plays a major role in shaping tropical rainforests and the response mechanisms are highly diverse and complex. The numerous gaps identified here require the international scientific community to combine efforts in order to conduct comprehensive studies in tropical rainforests on the three continents. These results are essential to simulate the future of these ecosystems under diverse climate scenarios and to predict the future of the global earth carbon balance. - Context: tropical rainforest ecosystems are characterized by high annual rainfall. Nevertheless, rainfall regularly fluctuates during the year and seasonal soil droughts do occur. Over the past decades, a number of extreme droughts have hit tropical rainforests, not only in Amazonia but also in Asia and Africa. The influence of drought events on tree mortality and growth or on ecosystem functioning (carbon and water fluxes) in tropical rainforest ecosystems has been studied intensively, but the response mechanisms are complex.- Aims: herein, we review the recent findings related to the response of tropical forest ecosystems to seasonal and extreme droughts and the current knowledge about the future of these ecosystems. - Results: this review emphasizes the progress made over recent years and the importance of the studies conducted under extreme drought conditions or in through-fall exclusion experiments in understanding the response of these ecosystems. It also points to the great diversity and complexity of the response of tropical rainforest ecosystems to drought. - Conclusion: the numerous gaps identified here require the international scientific community to combine efforts in order to conduct comprehensive studies in tropical forest regions. These results are essential to simulate the future of these ecosystems under diverse climate scenarios and to predict the future of the global earth carbon balance

Indoor optical wireless systems - A review

This paper reviews the research effort to date into optical wireless communication systems, both in academic and industrial contexts. The idea of using the optical medium for wireless communications is not new, having been proposed as a means for indoor communications almost two decades ago. However, the last few years have seen an explosive interest in the potential for free space optical systems to provide portable data communications. One of the prime motivators for reconsidering the use of an optical carrier in the wireless context is the demand for greater transmission bandwidths. The radio frequency spectrum is already exceedingly congested and frequency allocations of sufficient bandwidths are extremely hard to obtain. Further, for the high bandwidth services envisaged, the use of microwave or mm-wave systems will be required, where device technology is currently either expensive or immature. Proponents of optical wireless systems argue that the optical medium is the only cost-effective way to provide high bit-rate mobile services to volume markets. This paper identifies the technical obstacles and limitations of indoor optical wireless delivery in addition to techniques for mitigating these effects and shows that infrared is a viable alternative to radio for certain applications

Adjusted likelihood methods for modelling dispersion in generalized linear models

We consider the problem of modelling the failure-time distribution, where failure is due to two distinct causes. One approach is to adopt a two-component mixture model where the components correspond to the two different causes of failure. However, routine application of this approach with typical parametric forms for the component densities proves to be inadequate in modelling the time to a re-replacement operation or death after the initial replacement of the aortic valve in the heart by a prosthesis, such as a xenograft valve. Hence we consider modifications to the usual mixture model approach to handle situations where there exists a strong dependency between the failure times of the distinct causes. With these modifications, a suitable model is able to be provided for the distribution of the time to a re-replacement operation conditional on the age of the patient at the time of the initial replacement operation. The estimate so obtained by the probability that a patient of a given age will undergo a re-replacement operation provides a useful guide to heart surgeons on the type of valve to be used in view of the patient's age. Copyright (C) 1999 John Wiley & Sons, Ltd

High speed wireless IR-LANs using spatial addressing

Currently, there is considerable interest in infrared as an alternative medium to radio for indoor wireless communications, particularly for computer networking applications. This paper describes a novel IR-LAN link topology that employs an optical spatial-angular mapping technique which minimizes link losses and facilitates high link rates, whilst maintaining a useful coverage area. Blocking probability effects and overall system performance are examined in terms of aggregate transmission rates

Aortic-Valve Infection - Risk-Factors for Death and Recurrent Endocarditis After Aortic-Valve Replacement

Patients (n = 195) undergoing aortic valve replacement (n = 209) for native or prosthetic valve endocarditis were studied to determine risk factors for death and recurrent endocarditis and also to determine the valve type least likely to be associated with recurrent endocarditis. Ten-year survival was 60 %, the highest risk of dying occurring within the first 3 postoperative months. Risk factors for death in this early phase included increased urea concentration, higher New York Heart Association functional class, prosthetic valve endocarditis, infection status (lower in patients with healed endocarditis), longer duration of cardiopulmonary bypass, and nonuse of an allograft valve. In the late phase (beyond 3 months), risk factors included age at operation and Staphylococcus aureus infection (only in New York Heart Association functional class V). Ten years after aortic valve replacement, 79 % of valves were free of recurrent endocarditis. The highest risk of recurrence was in the first 4 months. Longer duration of cardiopulmonary bypass was a weak risk factor for recurrent endocarditis in the early phase, and in the late phase risk factors were S. aureus infection (only in New York Heart Association functional classes III, IV, and V) and the use of now discontinued biologic valves. Allograft aortic valve replacement was shown to be associated with a low and constant risk of recurrent endocarditis, whereas other valve types were associated with a high early risk. The allograft valve should be the preferred replacement device for aortic root infection

An Analysis of Risk-Factors for Death and Mode-Specific Death After Aortic-Valve Replacement with Allograft, Xenograft, and Mechanical Valves

From September 1967 to January 1990, a total of 2100 patients underwent 2366 aortic valve replacements with a variety of allograft, xenograft, and mechanical valves. Concomitant procedures were performed in 764 patients. Actuarial survival at 12 years was 59.6% (70% confidence limits 57.8% to 61.4%). Hazard function for death was highest immediately after operation, falling to merge with a slowly rising phase of risk at approximately 3 months. Actuarial freedom from sudden death at 12 years was 88.0% (70% confidence limits 86.7% to 89.3%). The shape of the hazard function for sudden death was similar to that for death. Actuarial freedom from death with cardiac failure at 12 years was 87.9% (70% confidence limits 86.5% to 89.2%). The shape of the hazard function for death with cardiac failure was also similar to that for death. Risk factor analysis revealed the important deleterious impact on long-term survival resulting from impaired left ventricular structure and function because of aortic valve disease. No current-era valve used in this study (allograft, xenograft, or mechanical) was a risk factor for death. Both aortic wall disease and endocarditis necessitating aortic valve replacement substantially decreased long-term patient survival. Aortic valve replacement is advisable much earlier in the natural history of aortic valve disease before secondary left ventricular damage occurs

Mode coupling effects in ring-core fibres for space-division multiplexing systems

An optical fibre with weak mode coupling is desirable for future ultra-high capacity space-division multiplexing (SDM) systems because mode coupling in an optical fibre results in extrinsic loss of the fibre and crosstalk between guided optical modes. To study the feasibility of a ring-core fibre (RCF) for SDM systems, in this paper, we investigate the mode coupling in the RCF supporting 5 or 7 guided mode groups (MGs) at a wavelength of 1550nm. For this purpose, the coupled mode/power theory (CMT/CPT) with identified spatial power spectrum of random perturbations of fibre axis is used to estimate the bend loss/crosstalk of the RCF due to microbending. It is shown that, based on the identified parameters for the spatial power spectrum in the 5/7-MG RCF, the estimated bend loss/crosstalk of the RCF agrees well with experimental results. In addition, the impact of the gradient parameter α and refractive index contrast Δ of the fibre refractive index profile on bend loss and crosstalk of the RCF is explored. Simulation results indicate that the Δ instead of the α significantly affects bend loss and crosstalk of the RCF. The magnitude improvement in bend loss by increasing the Δ is dependent on the spatial power spectrum