Normal Aerobic Vaginal Bacterial Flora of The African Giant Rats (AGR) Captured from Their Natural Habitat in Abeokuta, Ogun State, Nigeria

The genital tract of female animals, especially the caudal region, is known to habour non specific bacteria that are sometimes called the normal bacterial flora. In this study, we examined 12 apparently healthy female African giant rats (Cricetomys gambianus, Waterhouse) (AGR) to determine their vaginal bacterial flora. Swab collected from the vagina of each rat after previous chloroform anaesthesia was seeded onto blood and MacConkey agar plates and incubated aerobically at 370C for up to 48hours. Isolates were then characterized using various character parameters. The results indicated that 7 bacterial genera inhabit the vagina of the 12 African giant rats that were studied. The distribution of the bacteria species in the AGR were highlighted in the text. It is inferred from the results that under stress condition, these bacteria could cause disease in the African giant rats

COMPARATIVE MORPHOLOGY OF HAEMOCYTE FROM TWO SPECIES OF GIANT AFRICAN LAND SNAILS (Archachatina marginata AND Achatina achatina)

A study was conducted to evaluate the presence and types of haemocytes inherent in haemolymph of two species of giant African land snails (Archachatina marginata and Achatina achatina). Haemolymph samples were obtained from three liveweight groups of snails (< 100 g, 101-150 g and >150 g) after removal of the first three whorls of the shell. Smears were made from thin layer of settled portion of the haemolymph after six hours. Staining was carried out after air drying at room temperature with MayGrünwald-Giemsa stain.  Four replicates per liveweight per species were used. Representative slides were selected after viewing under microscope. Dimensions of haemocytes identified were taken followed by photomicrograph. Results showed that four haemocyte types were present in the haemolymph of both species. Those haemocytes identified in Archachatina marginata had significantly (P<0.001) higher dimensions than those found in Achatina achatina. Morphologically, the haemocytes were different in terms of shapes, nucleus position and cytoplasm types. It can therefore be concluded from this study that four circulating haemocytes types are present in both Archachatina marginata and Achatina achatina. Furthermore, in terms of dimension, Archachatina marginata had higher values compared to Achatina achatina. Also, morphologically, those four haemocyte types are different from each other in both specie of giant African land snails.Â

ANTIBACTERIAL ACTIVITIES OF HONEY FROM DIFFERENT LOCATIONS IN THE TROPICAL RAIN FOREST BELT OF NIGERIA, ON SELECTED BACTERIAL ISOLATES

Honey has been used for various purposes including treatment of diseases. This study was carried out to determine antibacterial activities of honey samples obtained from different locations in the tropical Rainforest Belt of Nigeria (Abeokuta, Aiyetoro, Ajebo, and Saki) against reference bacterial isolates: Escherichia coli (E.coli) (ATCC 25922) and Staphylococcus aureus (ATCC 25923), using agar well diffusion technique. Staphylococcus aureus showed sensitivity to all honey samples used with the zone of inhibition ranging between 1.9 and 2.6mm. Escherichia coli was resistant to all concentrations of honey samples used. However, the present study did not explore the possible causative agent(s) involved in the antibacterial activity of the honey used.Â

EVALUATION OF MATERNALLY-DERIVED ANTIBODIES AGAINST NEWCASTLE DISEASE VIRUS IN DAY-OLD CHICKS IN ABEOKUTA, OGUN STATE

High maternally-derived antibodies (MDA) against Newcastle disease virus (NDV) in chickens can interfere with active immunity at early age. This study was conducted to assess the titre of MDA in chicks against NDV from two poultry breeder farms. Twenty-six blood samples were collected from day-old chicks randomly selected from two poultry breeding farms and forty blood samples were collected from the parent stocks of the same farms. The vaccination record of the parent stocks were collected while the antibody titres of the birds were estimated using Haemagglutination Inhibition (HI) test. The geometric mean titre (GMT) of MDA against NDV in chicks from Farms 1 and 2 were log 27.2 and log 27.4 respectively while the parent stocks from Farms 1 and 2 had log 27.7 and log 27.9HI units. The percentage of chicks from Farms 1 and 2 with MDA titre above protective level (>log 23) were 90% and 100% respectively. It was concluded that MDA titres against ND virus was high in the chicks therefore vaccination at day one in the hatcheries and farms should be done after the immune status of the chicks are known since high MDA titre during vaccination neutralizes vaccine virus preventing active immunity.Â

ANTIMICROBIAL RESISTANCE IN ENTEROBACTERIACEAE FROM INTENSIVELY-REARED APPARENTLY HEALTHY AND DISEASED POULTRY IN ABEOKUTA, NIGERIA

The emergence and wide-spread dissemination of antimicrobial resistant bacteria strains is a global phenomenon of great public health and economic implications. Antimicrobial resistance was investigated in enterobacteriaceae isolated from apparently healthy and diseased poultry birds using the broth micro-dilution method to determine antimicrobial minimum inhibitory concentration (MIC). In all, 504 bacterial isolates including Escherichia coli (471), Klebsiella spp (28) and Salmonella enterica isolates (5) were studied. The isolates were resistant to ampicillin (88.5%), chloramphenicol (62.3%), ciprofloxacin (74.8%), enrofloxacin (81.0%), neomycin (83.9%), norfloxacin (78.8%), streptomycin (91.3%) and tetracycline (83.3%). The geometric mean MIC (µg/µL) of tested antimicrobials for enterobacteriaceae is as follows: ampicillin (102.5), chloramphenicol (48.4), ciprofloxacin (19.1), enrofloxacin (34.5), neomycin (47.7), norfloxacin (24.5), streptomycin (142.2) and tetracycline (62.5). Although rates of resistance to ampillin, streptomycin and tetracycline were similar among isolates from apparently healthy and diseases birds, resistance to chloramphenicol, ciprofloxacin, enrofloxacin, neomycin and norfloxacin were significantly higher (p<0.05) in isolates from diseased chickens than in those from apparently healthy chickens. The high rates of antimicrobial resistance in bacteria may contribute to the persistence of pathogens in poultry flock and ineffectiveness of antimicrobial chemotherapy during disease outbreaks.Â

Kinetics study of biologically remediated crude oil polluted soil using a bacteria and fungi

The optimum conditions for the fermentation of a local legume known as African locust bean (Parkia biglobosa) into a vegetable protein based food condiment or spice (Iru) were developed using Levenberg-Marquardt (or Powell) method (using PSI software) with three (3) variables namely; inoculum concentration (bacillus subtilis), temperature and the fermentation duration. P. biglobosa seeds were fermented at various temperature of 40 - ��� ��� �� � � ��� ���� �������� ���� ��� ��� ����������� �� ������� Inoculum. The proximate analysis shows that fermentation increased the percentage protein. Protein had the highest composition with about 51 % after 72 hours at the lowest �� ������ �������� ��� ���������

Phenomenological Model Development of Percentage Protein Present in Fermented African Locust Beans Seed

The optimum conditions for the fermentation of a local legume known as African locust bean (Parkia biglobosa) into a vegetable protein based food condiment or spice (Iru) were developed using Levenberg-Marquardt (or Powell) method (using PSI software) with three (3) variables namely; inoculum concentration (bacillus subtilis), temperature and the fermentation duration. P. biglobosa seeds were fermented at various temperature of 40 - ��� ��� �� � � ��� ���� �������� ���� ��� ��� ����������� �� ������� Inoculum. The proximate analysis shows that fermentation increased the percentage protein. Protein had the highest composition with about 51 % after 72 hours at the lowest �� ������ �������� ��� ���������

Assessment of early-maturing maize hybrids and testing sites using GGE biplot analysis

Article Purchased; Published: 7 Sept. 2017Identification of outstanding maize (Zea mays L.) hybrids for target environments is complicated by genotype × environment interactions. Thirty-two early-maturity maize hybrids were evaluated at eight locations in Nigeria and six locations in Ghana for 2 yr to (i) identify high-yielding, stable hybrids across locations and/or hybrids specifically adapted to different locations, and (ii) identify ideal test sites for selection of superior hybrids in the two countries. Genotype, country, year, location (country), and their interactive effects were significant (P < 0·01) for grain yield, days to anthesis and silking, anthesis-silking interval, plant and ear aspects, and ears per plant. Mean grain yield of the hybrids ranged from 3177 kg ha−1 for EWH-5 to 4596 kg ha−1 for EWH-29. The genotype main effects plus genotype × environment interaction (GGE) biplot analysis revealed that EWH-29, EWH-8, and EWH-30 did not differ significantly in grain yield and were the most stable hybrids in both countries, whereas EWH-26 and EWH-32 were the most stable hybrids only in Ghana. The GGE biplot analysis identified Samaru, Kafin Soli, and Minjibir in Nigeria and Nyankpala, Damongo, and Fumesua in Ghana as the most discriminating locations. Minjibir (Nigeria) and Nyankpala (Ghana), being most discriminating and representative locations, were considered the ideal testing sites for the respective countries. The type of cultivars evaluated determined the most suitable locations for multilocation testing within Ghana and Nigeria. Whereas hybrids EWH-29, EWH-8, and EWH-30 should be suitable for production in Nigeria, EWH-26 and EWH-32 should be promoted for production in Ghana

Stability analysis of maize cultivars adapted to tropical environments using AMMI analysis

Genotype × environment interactions complicate selection of superior genotypes for narrow and wide adaptation. Eighteen tropically-adapted maize cultivars were evaluated at six locations in Nigeria for 2 yrs to (i) identify superior and stable cultivars across environments and (ii) assess relationships among test environments. Environment and genotype × environment interactions (GEI) were significant (P −1 for TZL COMP4 C3 DT C2 with an average of 2555 kg ha−1. Cultivar DT SYN2-Y had the least additive main effect and multiplicative interaction (AMMI) stability value of 7.4 and hence the most stable but low-yielding across environments. AMMI biplot explained 90.5% and classified cultivars and environments into four groups each. IWD C3 SYN F3 was identified as the high-yielding and stable cultivar across environments. ZA15, ZA14, BK14, BK15 and IL15 had environment mean above the grand mean, while BG14, BG15, LE14, LE15, IL14, LA14 and LA15 had mean below the grand mean. ZA, BK, BG, LE and LA were found to be consistent in ranking the maize cultivars. However, Zaria, Birnin Kudu, and Ilorin were identified as the best test locations and could be used for selecting the superior maize cultivars. The identified high-yielding and stable cultivar could be further tested and promoted for adoption to contribute to food insecurity in Nigeria

Genetic variances and relationship among traits of an early maturing maize population under drought-stress and low nitrogen environments

Article purchasedDrought and low soil nitrogen (low N) are major causes of low grain yield of maize (Zea mays L.) in sub-Saharan Africa (SSA). An early maturing maize population, TZE-Y Pop DT STR, had undergone four cycles of selection for drought tolerance, followed by four selection cycles for resistance to Striga hermonthica (Del.) Benth., which is normally conducted under low N (about 30 kg N ha-1). The objectives of this study were to estimate residual genetic variances, predict future gains from selection, and investigate inter-trait relationships in the population under drought-stress, low N and across research environments. North Carolina Design I was used to develop 250 full-sib progenies from the improved population, which were evaluated in three drought-stress and two low N environments in Nigeria, 2011 to 2013. Additive genetic variances were not significant for most traits under the research conditions. The predicted gains from selection for grain yield were 5.3, 8.5 and 7.5% cycle-1 under drought, low N, and across environments. These results suggested the absence of substantial genetic variability in the population to ensure progress from selection. Ears per plant (EPP), ear aspect (EASP), plant aspect (PASP), and stay green characteristic (STGR) were consistently identified as important secondary traits under both research conditions. We concluded that there is need to introgress new sources of favorable alleles for drought-stress and low N tolerance into the population for guaranteed progress from selection, using EPP, EASP, PASP, and STGR in combination with yield in a selection index under drought-stress and low N