Hemi-Synthesis and Anti-Oomycete Activity of Analogues of Isocordoin

Indexación: Web of Science; Scopus.An efficient synthesis of a series of 4-oxyalkyl-isocordoin analogues (2–8) is reported for the first time. Their structures were confirmed by1H-NMR,13C-NMR, and HRMS. Their anti-oomycete activity was evaluated by mycelium and spores inhibition assay against two selected pathogenic oomycetes strains: Saprolegnia parasitica and Saprolegnia australis. The entire series of isocordoin derivatives (except compound 7) showed high inhibitory activity against these oomycete strains. Among them, compound 2 exhibited strong activity, with minimum inhibitory concentration (MIC) and minimum oomyceticidal concentration (MOC) values of 50 µg/mL and 75 µg/mL, respectively. The results showed that 4-oxyalkylated analogues of isocordoin could be potential anti-oomycete agents.http://www.mdpi.com/1420-3049/22/6/96

The physical structure of the point-symmetric and quadrupolar planetary nebula NGC 6309

We analyse the point-symmetric planetary nebula NGC 6309 in terms of its three-dimensional structure and of internal variations of the physical conditions to deduce the physical processes involved in its formation. We used VLA-D 3.6-cm continuum, ground-based, and HST-archive imaging as well as long slit high- and low-dispersion spectroscopy. The low-dispersion spectra indicate a high excitation nebula, with low to medium variations of its internal physical conditions. In the optical images, the point-symmetric knots show a lack of [NII] emission as compared with similar features previously known in other PNe. A rich internal structure of the central region is seen in the HST images, resembling a deformed torus. Long slit high-dispersion spectra reveal a complex kinematics in the central region. The spectral line profiles from the external regions of NGC 6309 indicate expanding lobes (~40 km/s) as those generally found in bipolar nebulae. Finally, we have found evidence for the presence of a faint halo, possibly related to the envelope of the AGB-star progenitor. Our data indicate that NGC 6309 is a quadrupolar nebula with two pairs of bipolar lobes whose axes are oriented PA=40 and PA=76. Equatorial and polar velocities for these two pairs of lobes are 29 and 86 km/s for the bipolar system at PA=40 and 25 and 75 km/s for the bipolar system at PA=76. There is also a central torus that is expanding at 25 km/s. Kinematical age for all these structures is around 3700 to 4000 yr. We conclude that NGC 6309 was formed by a set of well-collimated bipolar outflows (jets), which were ejected in the initial stages of its formation as a planetary nebula. These jets carved the bipolar lobes in the previous AGB wind and their remnants are now observed as the point-symmetric knots tracing the edges of the lobes.Comment: To be published in Astronomy & Astrophysics; 11 pages, 8 figures, 4 table

Optical studies of gap, hopping energies and the Anderson-Hubbard parameter in the zigzag-chain compound SrCuO2

We have investigated the electronic structure of the zigzag ladder (chain) compound SrCuO2 combining polarized optical absorption, reflection, photoreflectance and pseudo-dielectric function measurements with the model calculations. These measurements yield an energy gap of 1.42 eV (1.77 eV) at 300 K along (perpendicular) to the Cu-O chains. We have found that the lowest energy gap, the correlation gap, is temperature independent. The electronic structure of this oxide is calculated using both the local-spin-density-approximation with gradient correction method, and the tight-binding theory for the correlated electrons. The calculated density of electronic states for non-correlated and correlated electrons shows quasi-one-dimensional character. The correlation gap values of 1.42 eV (indirect transition) and 1.88 eV (direct transition) have been calculated with the electron hopping parameters t = 0.30 eV (along a chain), t_yz = 0.12 eV (between chains) and the Anderson-Hubbard repulsion on copper sites U= 2.0 eV. We concluded that SrCuO_2 belongs to the correlated-gap insulators.Comment: 24 pages, 8 figures, to be published in Phys.Rev.

Validación de un sistema de medición aplicado a un estudio de termografía sensorial para la detección de desórdenes de traumas acumulados

En el presente trabajo se muestra la aplicación del estudio de Repetibilidad y Reproducibilidad (R y R), realizado para evaluar el Sistema de Medición (SM) utilizado en un estudio de termografía sensorial en seres humanos, para la detección de Desórdenes de Traumas Acumulados (DTA’s). La termografía es una técnica no invasiva en los seres humanos y que no los dañará en ningún momento. La termografía sensorial, a diferencia de la infrarroja, basa su funcionamiento en monitoreo por sensores para capturar temperaturas y buscar anomalías que pudieran dar indicio en el desarrollo de enfermedades. Por tal motivo la confiabilidad al obtener temperaturas es vital. Para ello se seleccionó al estudio R y R como herramienta para la validación del sistema de medición.Este estudio hace un análisis comparativo en la validación del sistema de medición que ayudará a predecir DTA’s; utilizando en el primer experimento a dos operadores que manejan el instrumento de medición y toman las lecturas a diez personas, las cuales son consideradas como las diez partes diferentes a que hace referencia la literatura en los estudios R y R clásicos, en las cuáles se mide en dos ocasiones diferentes su temperatura cutánea en el área de la muñeca en mano derecha por cada operador. Y después, en un segundo experimento, se utilizan tres operadores y diez personas, midiéndose en tres ocasiones diferentes su temperatura en la misma región que el primer experimento. Los resultados obtenidos en esta aplicación por los dos experimentos mostraron -en general-, que el sistema de medición es excelente según los parámetros establecidos por la Automobile Industries Action Group (AIAG), lo que permite seguir adelante con las otras fases de la investigación principal.This paper shows the implementation of the study of Repeatability and Reproducibility (R & R) conducted to evaluate the Measurement System (MS) used in a study of sensorial thermography in humans, to detect Cumulatives Traumas Disorders (CTD’s). Thermography is a noninvasive technique on humans and not harm at all times. Thermography unlike sensory bases its operation on infrared sensors to capture monitoring temperatures and look for abnormalities that could give evidence in the development of disease. For this reason reliability is vital to obtain temperatures for this study was selected R & R as a tool for the validation of the measurement system.This study makes a comparative analysis in the validation of the measurement system to help to predict CTD’s, used in the first experiment two operators who handle the meter and take readings at ten persons, which are considered as ten different parts referred to literature in the conventional R & R studies, in which is measured at two different times their skin temperature in the area of the right wrist by each operator. And then, in a second experiment using three operators and ten persons, measured on three different occasions the temperature in the same region as the first experiment. The results obtained in this application by the two experiments showed, in general, the measurement system is excellent according to parameters established by the Automobile Industries Action Group (AIAG), which allows proceeding with the other phases of primary research

I–II Loop Structural Determinants in the Gating and Surface Expression of Low Voltage-Activated Calcium Channels

The intracellular loops that interlink the four transmembrane domains of Ca2+- and Na+-channels (Cav, Nav) have critical roles in numerous forms of channel regulation. In particular, the intracellular loop that joins repeats I and II (I–II loop) in high voltage-activated (HVA) Ca2+ channels possesses the binding site for Cavβ subunits and plays significant roles in channel function, including trafficking the α1 subunits of HVA channels to the plasma membrane and channel gating. Although there is considerable divergence in the primary sequence of the I–II loop of Cav1/Cav2 HVA channels and Cav3 LVA/T-type channels, evidence for a regulatory role of the I–II loop in T-channel function has recently emerged for Cav3.2 channels. In order to provide a comprehensive view of the role this intracellular region may play in the gating and surface expression in Cav3 channels, we have performed a structure-function analysis of the I–II loop in Cav3.1 and Cav3.3 channels using selective deletion mutants. Here we show the first 60 amino acids of the loop (post IS6) are involved in Cav3.1 and Cav3.3 channel gating and kinetics, which establishes a conserved property of this locus for all Cav3 channels. In contrast to findings in Cav3.2, deletion of the central region of the I–II loop in Cav3.1 and Cav3.3 yielded a modest increase (+30%) and a reduction (−30%) in current density and surface expression, respectively. These experiments enrich our understanding of the structural determinants involved in Cav3 function by highlighting the unique role played by the intracellular I–II loop in Cav3.2 channel trafficking, and illustrating the prominent role of the gating brake in setting the slow and distinctive slow activation kinetics of Cav3.3

Orientation of the Calcium Channel β Relative to the α12.2 Subunit Is Critical for Its Regulation of Channel Activity

BACKGROUND: The Ca(v)beta subunits of high voltage-activated Ca(2+) channels control the trafficking and biophysical properties of the alpha(1) subunit. The Ca(v)beta-alpha(1) interaction site has been mapped by crystallographic studies. Nevertheless, how this interaction leads to channel regulation has not been determined. One hypothesis is that betas regulate channel gating by modulating movements of IS6. A key requirement for this direct-coupling model is that the linker connecting IS6 to the alpha-interaction domain (AID) be a rigid structure. METHODOLOGY/PRINCIPAL FINDINGS: The present study tests this hypothesis by altering the flexibility and orientation of this region in alpha(1)2.2, then testing for Ca(v)beta regulation using whole cell patch clamp electrophysiology. Flexibility was induced by replacement of the middle six amino acids of the IS6-AID linker with glycine (PG6). This mutation abolished beta2a and beta3 subunits ability to shift the voltage dependence of activation and inactivation, and the ability of beta2a to produce non-inactivating currents. Orientation of Ca(v)beta with respect to alpha(1)2.2 was altered by deletion of 1, 2, or 3 amino acids from the IS6-AID linker (Bdel1, Bdel2, Bdel3, respectively). Again, the ability of Ca(v)beta subunits to regulate these biophysical properties were totally abolished in the Bdel1 and Bdel3 mutants. Functional regulation by Ca(v)beta subunits was rescued in the Bdel2 mutant, indicating that this part of the linker forms beta-sheet. The orientation of beta with respect to alpha was confirmed by the bimolecular fluorescence complementation assay. CONCLUSIONS/SIGNIFICANCE: These results show that the orientation of the Ca(v)beta subunit relative to the alpha(1)2.2 subunit is critical, and suggests additional points of contact between these subunits are required for Ca(v)beta to regulate channel activity

Rest-Mediated Regulation of Extracellular Matrix Is Crucial for Neural Development

Neural development from blastocysts is strictly controlled by intricate transcriptional programmes that initiate the down-regulation of pluripotent genes, Oct4, Nanog and Rex1 in blastocysts followed by up-regulation of lineage-specific genes as neural development proceeds. Here, we demonstrate that the expression pattern of the transcription factor Rest mirrors those of pluripotent genes during neural development from embryonic stem (ES) cells and an early abrogation of Rest in ES cells using a combination of gene targeting and RNAi approaches causes defects in this process. Specifically, Rest ablation does not alter ES cell pluripotency, but impedes the production of Nestin+ neural stem cells, neural progenitor cells and neurons, and results in defective adhesion, decrease in cell proliferation, increase in cell death and neuronal phenotypic defects typified by a reduction in migration and neurite elaboration. We also show that these Rest-null phenotypes are due to the dysregulation of its direct or indirect target genes, Lama1, Lamb1, Lamc1 and Lama2 and that these aberrant phenotypes can be rescued by laminins