19F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy

Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues using microscopy, to enable a proper comparison of results over these different scales but, often, this is not feasible. The cell surface and extracellular matrix polysaccharides, glycosaminoglycans (GAGs) lack groups that can be detected selectively in the biological milieu. The introduction of 19F labels into GAG polysaccharides is explored and the interaction of a labelled GAG with the heparin-binding protein, antithrombin, employing 19F NMR spectroscopy is followed. Furthermore, the ability of 19F labelled GAGs to be imaged using CARS microscopy is demonstrated. 19F labelled GAGs enable both 19F NMR protein-GAG binding studies in solution at the molecular level and non-linear microscopy at a microscopic scale to be conducted on the same material, essentially free of background signals

CHEMOTHERAPY CARDIOTOXICITY SCREENING WITH CARDIAC TROPONINS

Recently, chemotherapy is one of the most trustworthy methods for treatment of malignant tumors and blood pathology. However clinical effect of the drugs not always matches with safety. These facts play as a solid evidence for the necessity of studying the problems related to side effects of the drugs, and, particularly, of cardiotoxicity, as reversible and/or irreversible damage of cardiomyocytes. Currently, the most sensitive ad specific markers of myocardium cells are troponins T and I. According with this, the main aims of this article are analysis of literary data for the problem of cardiac troponins application as cardiotoxicity revealing in oncology patients, and summon of more attention to this direction of science. The article provides a collection of the key points of all available studies at the moment, shows generalized analysis, analyses also the Russian and European Guidelines from the point of view related to troponins tests in oncology patients

THE STUDY "REGISTER OF PATIENTS AFTER ACUTE STROKE (REGION)." Part 1. Hospital Prospective Register of Patients after Acute Stroke (According to the Results of the Pilot Phase of the Study)

Aim. To assess the main features of the clinical course of acute cerebrovascular accident (ACVA), its short-term and long-term outcomes and quality of pharmacotherapy based on hospital register. Material and methods. The hospital register of acute stroke (AS) was organized in one of the cardiovascular centers in Moscow city. The results of the pilot part of the study are presented (170 patients hospitalized from January 01, 2014 to September 30, 2014 with ACVA living in the service area of one of the closest outpatient clinics). Presence of cardiovascular diseases (CVD) and their risk factors (RF), prehospital therapy, short-term complications including death and pharmacotherapy recommended to survived patients were analyzed using hospital medical records. During ambulatory follow-up (prospective part of the register) the vital status and pharmacotherapy were assessed. Results. The majority of patients with AS had concomitant CVD (on average 2 per patient) and non CVD (on average 1.2 per patient). Data on the risk factors of CVD and their complications were reflected insufficiently in the medical records. Most patients in the prehospital period did not receive adequate treatment for the reduction in the cardiovascular risk. 90 patients survived and were discharged. 1.5-2 years after discharge, information on the vital status was available for 78 (86.7%) patients. 61 of them (78.2%) were alive and 17 (21.8%) died. Conclusion. The pilot part of the REGION register revealed that the majority of patients with AS have concomitant CVD and non-CVD. The overall quality of pharmacotherapy, primary and secondary prevention of ACVA was far from that recommended in clinical guidelines, especially during follow-up in outpatient clinic