Научно-образовательная и организаторская деятельность профессора М. И. Стуковенкова

У статті висвітлюються основні віхи життя та діяльності визначного дерматовенеролога, директора київської дерматологічної клініки ординарного професора імператорського університету Святого Володимира М.І. Стуковенкова за період 1842–1897 рр.The article highlights the most significant achievements of an eminent scientist, dermatovenereologist, Director of Kiev dermatological clinic, Professor of St. Vladimir imperial university M.I. Stukovenkov (1842 – 1897).В статье освещаются основные вехи жизни и деятельности выдающегося дерматовенеролога, директора киевской дерматологической клиники, ординарного профессора императорского университета Св. Владимира М. И. Стуковенкова (за период 1842 – 1897 гг.)

Науково-освітня та організаційна діяльність професора М.І. Стуковенкова

У статті висвітлюються основні віхи життя та діяльності визначного дерматовенеролога, директора київської дерматологічної клініки ординарного професора імператорського університету Святого Володимира М.І. Стуковенкова за період 1842–1897 рр

БАТАРЕЙНЫЕ СИСТЕМЫ НАКОПЛЕНИЯ ЭЛЕКТРИЧЕСКОЙ ЭНЕРГИИ ДЛЯ ИСПОЛЬЗОВАНИЯ В ИНТЕЛЛЕКТУАЛЬНЫХ ЭНЕРГЕТИЧЕСКИХ СИСТЕМАХ

The main features of battery electric energy storage systems (BESS) are considered in this paper . The functions and the recommended range of installed capacity of BESS are specified depending on their point of connection to energy system. The topologies of a hybrid battery system, which may be used in a range of small, medium and large capacity are given. Different control strategies for hybrid storage system that provide the recommend charge/discharge times of BESS elements are presented. References 30, figures 7.Рассмотрены особенности батарейных систем накопления электрической энергии (БСНЭ). Указаны функции БСНЭ, диапазон рекомендуемой установленной мощности и энергоемкости систем в зависимости от точки их подключения. Приведены структурные схемы гибридных батарейных систем, которые могут применяться в диапазоне малых, средних и больших мощностей. Предложен метод управления гибридной системой накопления, обеспечивающий работу элементов БСНЭ с рекомендуемыми временами заряда-разряда. Библ. 30, рис. 7.Розглянуто особливості батарейних систем накопичення електричної енергії (БСНЕ). Вказані функції БСНЕ, діапазон встановленої потужності та енергоємності систем, що рекомендується в залежності від точки їх підключення. Наведено структурні схеми гібридних батарейних систем, що можуть використовуватись в діапазоні малих, середніх та великих потужностей. Запропоновано метод управління гібридною системою накопичення, що забезпечує роботу елементів БСНЕ з часом заряду-розряду, що рекомендується. Бібл. 30, рис. 7

Glycyrrhetinic acid and its derivatives as inhibitors of poly(ADP-ribose)polymerases 1 and 2, apurinic/apyrimidinic endonuclease 1 and DNA polymerase β

Aim. For strengthening the efficiency of monofunctional alkylating antineoplastic drugs it is important to lower the capacity of base excision repair (BER) system which corrects the majority of DNA damages caused by these reagents. The objective was to create inhibitors of the key BER enzymes (PARP1, PARP2, DNA polymerase β, and APE1) by the directed modification of glycyrrhetinic acid (GA). Methods. Amides of GA were produced from the GA acetate by formation of the corresponding acyl chloride, amidation with the appropriate amine and subsequent deacylation. Small library of 2-cyano substituted derivatives of GA methyl esters was obtained by the structural modification of GA framework and carboxylic acid group. The inhibitory capacity of the compounds was estimated by comparison of the enzyme activities in specific tests in the presence of compounds versus their absence. Results. None of tested compounds inhibits PARP1 significantly. Unmodified GA and its morpholinic derivative were shown to be weak inhibitors of PARP2. The derivatives of GA containing keto-group in 11 triterpene framework were shown to be moderate inhibitors of pol β. Compound 3, containing 12-oxo-9(11)-en moiety in the ring C, was shown to be a single inhibitor of APE1 among all compounds studied. Conclusions. The class of GA derivatives, selective pol β inhibitors, was found out. The selective inhibitor of APE1 and weak selective inhibitor of PARP2 were also revealed

The Urals as a vernaculars district

Урал рассматривается как особое символическое пространство. Выдвигается гипотеза о том, что этот регион является вернакулярным районом и существует в дискурсе, а его границы могут не совпадать с географическими и/или административными границами. Чтобы их выявить, проводится анализ региональных СМИ, выявляются упоминания принадлежности к Уралу.The author considers the Ural district as a special symbolic space. There is a hypothesis about this area as vernacular region. It exists in the framework of discourse, and its borders may not coincide with geographical and/or administrative boundaries. The authors analyze regional media and reveal references of belonging to the Urals.Исследование выполнено при финансовой поддержке РФФИ в рамках научного проекта № 18-312-00143 мол_а «Региональная идентичность россиян в дискурсе СМИ: единство и разнообразие»

Journalistic Practices of Construction Calendar Holidays: History, Person, Symbolic Meaning

В центре внимания статьи — конструирование реальности в медиадискурсе. Авторы исследуют дискурсивные практики конструирования календарных праздников (на примере Дня народного единства в России). Исследование показало, что журналисты пишут об истории и традициях праздника. Они также описывают праздничные мероприятия и цитируют поздравления официальных лиц. Авторы выявили такие важные дискурсивные практики, как придание празднику символического значения и связь исторических событий с текущей ситуацией.The article deals with the construction of reality in the media discourse. The authors research discursive practices of the construction of calendar holidays (for example, National Unity Day in Russia). The study showed that journalists write about the history and traditions of the holiday. They also describe celebratory events and quote congratulations from officials. The authors identified such important discursive practices as attributing a symbolic meaning to the holiday and the connection of historical events with the current situation

Modeling of the Process of Preparing a Bachelor of Professional Education in the Conditions of Pedagogical Practice on the Basis of a Competence-Based Approach

В статье рассмотрены вопросы организации подготовки будущего педагога профессионального обучения в процессе прохождения педагогической практики на основе компетентностно-ориентированного подхода.The article deals with the organization training of the future teacher of professional training in the process of passing pedagogical practice on the basis of a competence-based approach

Myeloid-derived suppressor cells as biomarkers of the effectiveness of therapy with new biological agents in axial spondyloarthritis

Innate immune cells, including myeloid cells — myeloid derived suppressor cells (MDSCs) — are supposed to play an important role in the pathogenesis of axial spondyloarthritis (AxSp). Myeloid derived suppressor cells represent a heterogeneous population of immature cells capable of suppressing innate and adaptive immune responses with the most pronounced suppressor activity against T cells. Biological disease-modifying antirheumatic drugs (bDMARDs) can reduce the clinical and laboratory disease activity, but their effectiveness varies widely in different patients with AxSp. The present study is aimed at studying MDSCs subpopulations and their suppressive function depending on the response to bDMARD therapy in AxSp. The study included AxSp patients with a disease duration of 16.5 years (median); HLA-B27 (+) status was detected in 79% of cases. All patients received bDMARDs at least the past 12 weeks, including TNF inhibitors (etanercept, certolizumab pegol, adalimumab, or golimumab) or IL-17 inhibitors (secukinumab, ixekizumab, or netakimab). Percentage of granulocytic MDSCs (G-MDSCs, Lin-HLA-DR-CD33+CD66b+), monocytic MDSCs (M-MDSCs, HLA-DRlow/-CD14+), MDSCs of early stage differentiation (E-MDSCs, Lin-HLA-DR- CD33+CD66b-), as well as intracellular expression of arginase-1 was assessed by flow cytometry. Frequency of circulating MDSC subpopulations of patients with a stable response to bDMARDs (responders) did not differ significantly compared to healthy donors. Patients not responding to bDMARDs therapy showed increased relative and absolute number of E-MDSCs compared to healthy donors (pU = 0.01 and pU = 0.02, respectively) and the responders (pU = 0.03 and pU = 0.07, respectively). Increased percentage of E-MDSCs was positively correlated to disease activity — ESR (Rs = 0.821; p = 0.023), CRP (Rs = 0.714; p = 0.07) and ASDASCRP (Rs = 0.829; p = 0.042) in the non-responder group. Responder patients exhibited no correlation between disease activity and circulating MDSCs. The suppressor potential of MDSCs was analyzed by the intracellular expression of arginase-1 molecule which is involved in the inhibition of T cell response. Patients with the stable response were characterized by increased expression of arginase-1 in E-MDSCs compared to donors (pU = 0.02). Non-responders did not demonstrate significant changes in Arg-1 expression, however, the percentage of arginase-1-expressing G-MDSCs was positively correlated to indexes ASDASESR (Rs = 0.857; p = 0.014) and BASDAI (Rs = 0.785; p = 0.036). Thus, E-MDSCs as well as arginase-1 expression in MDSCs may serve as biomarkers of effectiveness bDMARD therapy, and act as potential candidate predictors of response to therapy in AxSp

The Training of Students in a Professional-Pedagogical University on the Basis of Digital Site of Pedagogical Practice (Method - Webquest)

В статье рассматриваются возможности использования инновационной технологии обучения для формирования профессиональной компетентности студентов в профессиональном обучении на основе принципов цифровой дидактики. Предложены подходы к внедрению интерактивных методов в обучении на примере метода - WEBQUEST. Описаны условия реализации данного метода в условиях цифровой площадки педагогической практики.The article discusses the possibilities of using innovative teaching technology to form students' professional competence in vocational training based on the principles of digital didactics. Approaches to the introduction of interactive methods in training are proposed using the example of the method - WEBQUEST. The conditions for the implementation of this method in terms of remote access to the resources of the department are described

Studies of effector molecules exerting autonomous and nonautonomous influence of T lymphocyte apoptosis under the conditions of in vitro “cell neighborhood” in healthy people and patients with rheumatoid arthritis

Cellular homeostasis in the body is known to be maintained by the processes of cell proliferation and death, whereas apoptosis is the most frequent and physiological, “silent” mechanism of cell elimination. It has been currently shown that the process of apoptosis traditionally considered an autonomous event, has a pronounced non-autonomous effect on migration, proliferation, and death of the neighboring cells. This work was based on the data on impaired programmed death of mononuclear cells from the patients with rheumatoid arthritis (RA) leading to the evolving autoimmune inflammation. The aim of this study was to evaluate effector molecules exerting autonomous and non-autonomous influence of T cell apoptosis under the conditions of “cell neighborhood” in cell cultures of healthy people and RA patients. The studies were performed with blood samples of RA patients and healthy women of comparable age. These experiments were performed in order to assess the levels of main molecules mediating the in vitro receptor and mitochondrial apoptosis of T lymphocytes. In previous studies, using the original “cell neighborhood” model, no differences were found in parameters of early and late activation apoptosis between the groups of donors and RA patients. At the same time, 1-week incubation in apoptotic cultures of the patients was followed by significantly increased number of viable cells carrying the proliferation marker Ki-67. Different results of in vitro apoptosis induction in cultures under similar conditions of “cell neighborhood” in healthy people and patients with RA have revealed the importance of main effector molecules of apoptosis in the studied groups. In this study, we have revealed low potential of the receptor pathway for apoptosis activation in healthy people, due to suppression of TNFα production during cell incubation under the conditions of “cell neighborhood”, and in RA patients due to initially low TNFα in supernatants which did not change over time and in various incubation variants, along with low content of initiating caspase 8 in both groups. Significant suppression of effector molecules of mitochondrial pathway of apoptosis activation, i.e., Bcl-2 anti-apoptotic factor and p53 transcription factor was detected in cultures of apoptotic cells, as well as mixtures of proliferating and apoptotic cells under the conditions of “cell neighborhood” in RA patients. The amounts of these molecules did not change in healthy persons. At the same time, no differences in these molecules were found between individual variants of cell cultures from the patients with RA and healthy people. The both studied groups were characterized by a significant activation of IL-4 and IL-6 production, i.e., the cytokines with autonomous and non-autonomous protective and reparative properties, Hence, one may conclude that high levels of these cytokines had different effects in cell cultures under the conditions of “cell neighborhood”. Incubation of cells from healthy people under suboptimal conditions was associated with maintaining the balance of proliferation and apoptosis, whereas, in cell cultures of RA patients, this balance caused activation of proliferation processes, being accompanied by an increase in the number of living cells in apoptotic cultures