Psychomotor Development of Preschool Children By Means of Musical Improvisation

This article is a fragment of a research project devoted to the study of the impact of the systematical musical influence on the integrated mental development of children. In this paper, we present an unique approach to the use of a criteria-oriented system for assessing the levels of psychomotor development of preschool children. Three estimated levels (from high to low) of child’s psychomotor development have been singled out. The range of characteristics of each level reflects the varying formation degree of the main indicators of the child’s psychomotor development and allows to establish both actual features of this development and to predict potential opportunities. The analysis of the results of the ascertaining experiment revealed some motor failures in a significant number of children aged 6-7 years. The failures manifested themselves in: difficulties of performing movements in accordance with instructions; violation of motor coordination, motor memory, inability to perform movements in accordance with spatial, temporal and dynamic characteristics, in thedistortion of tempo, rhythm and amplitude of movements (32.15%). The development program ”Music of My Body” presented in the article is aimed at overcoming the psychomotor underdevelopment of children and bringing them to the level of optimally realized age opportunities by means of musical improvisation plastics.As a result of the program, the following tasks are solved: developing a sense of rhythm and motor skills, the formation of correct posture and expressiveness of the movements, and facial expressions of children. The results of the program revealed positive changes in the psychomotor development of children. As a result of the program, the number of preschool children who demonstrated the first level in the development of motor skills almost doubled (from 13.82% to 38.09% at the control stage). The number of children with a low level of psychomotor development decreased (from 32.15% to 13.09% at the control stage). The dynamics of development based on the results of the control phase was statistically significant at a high level of reliability (p <0.001). Keywords: psychomotor development, emotional and physical comprehension of music, ”sounding gestures”, improvisation, mimic intonation of music

Formation of Coordinating Capabilities of Junior Schoolboys on Sectional Employments by a Rhythmic Gymnastics

У статті на основі аналізу й узагальнення науково-методичної літератури висвітлені питання розвитку координаційних здібностей молодших школярів у процесі занять із ритмічної гімнастики, яка найбільш відповідає потребам, здібностям та можливостям дітей молодшого шкільного віку й має цінний педагогічний потенціал для фізичного виховання. In the article on the basis of analysis and generalization scientifically of methodical literature the questions of development of co-ordinating capabilities of junior schoolboys are lighted up in the process of employments of rhythmic gymnastics. A rhythmic gymnastics most answers necessities, capabilities and possibilities of children of midchildhood, and has valuable pedagogical potential for physical education

Metabolic Risk Factors and Their Impact on Quality of Life in Patients with Pancreatic Cancer, Acute or Exacerbated Chronic Pancreatitis

Аim: to evaluate metabolic risk factors and their impact on quality of life in patients with pancreatic cancer (PC) and in patients with acute or exacerbated chronic pancreatitis.Materials and methods. Forty-five patients with PC (group 1) and 141 patients with acute pancreatitis or exacerbated chronic pancreatitis (group 2) in an observational multicenter clinical cross-sectional uncontrolled study were examined. Clinical, laboratory and instrumental examination of patients and assessment of risk factors (lipid profile, blood plasma glucose, obesity, arterial hypertension) were carried out in accordance with clinical recommendations. Patients completed the SF-36 questionnaire once to assess quality of life at hospital admission before treatment.Results. In group 1, indicators of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) in blood serum (3.7 ± 0.2; 2.2 ± 0.2 and 0.8 ± 0.1 mmol/L) were lower than in group 2 (5.1 ± 0.1; 3.1 ± 0.1 and 1.2 ± 0.1 mmol/L; p < 0.05). Arterial hypertension was more common in group 1 (55.6 %) than in group 2 (34.8 %; p = 0.013). The presence of arterial hypertension increases the chance of having PC by 2.7 times (p < 0.05). Body mass index parameters, including obesity, as well as parameters of triglycerides, and fasting plasma glucose, did not differ between the groups. Logistic regression analysis revealed a direct relationship with PC HDL hypocholesterolemia (Exp B = 4.976; p < 0.001) and arterial hypertension (Exp B = 2.742; p = 0.027) and an inverse relationship — with hypercholesterolemia (Exp B = 0.204; p = 0.002). The chance of having PC was not associated with age, fasting plasma glucose ³ 7.0 mmol/L, obesity. Quality of life indicators were higher in group 1 than in group 2 on four SF-36 scales: bodily pain (68.1 ± 5.1 and 36.8 ± 2.0; p < 0.001), general health (51.1 ± 2.5 and 38.0 ± 1.7 points; p < 0.001), social functioning (74.7 ± 3.0 and 64.5 ± 2.2 points; p = 0.007), role emotional functioning (28.2 ± 5.2 and 12.5 ± 3.1 points; p = 0.007) and in the general domain “physical component of health” (40.2 ± 1.0 and 33.6 ± 0.8 points; p < 0.001). In group 1 with HDL hypocholesterolemia compared with its absence, the indicators of role emotional functioning (22.2 ± 5.1 and 51.9 ± 13.7 points; p = 0.020) were lower, with arterial hypertension compared with its absence — role physical functioning (5.0 ± 4.0 and 25.5 ± 7.5 points; p = 0.036) and role emotional functioning (16.0 ± 5.1 and 43.3 ± 8.8 points; p = 0.007) were lower.Conclusions. In patients with PC arterial hypertension was more common and the levels of total cholesterol, LDL-C and HDL-C were lower than in patients with acute or exacerbated chronic pancreatitis. The chance of having PC is directly associated with HDL hypocholesterolemia, with arterial hypertension, inversely — with hypercholesterolemia, and is not associated with age, fasting plasma glucose ³ 7 mmol/L, or obesity. In patients with PC, quality of life indicators were higher on four SF-36 scales and on the general domain “physical component of health” than in the group with acute or exacerbated chronic pancreatitis. In patients with PC metabolic factors significantly worsened self-assessment of quality of life in terms of role functioning; in patients with acute or exacerbated chronic pancreatitis there was no such association

Development of a microchip analytical system with PCR reagents lyophilized into aluminum microchips and modified by the plasma enhanced chemical vapor deposition

Микрочиповые аналитические системы для молекулярно-генетического анализа в последние годы привлекают повышенный интерес исследователей. Алюминиевые микрочипы являются особенно перспективными для внедрения микрочипового формата полимеразной цепной реакции (ПЦР) в лабораторную практику. Подобные микрочипы требуют пассивации поверхности для предотвращения ингибирования ПЦР и улучшения аналитических характеристик системы. Привлекательна и возможность снижения трудозатрат и ошибок оператора за счет лиофилизации реактивов в микрочипах. Микрочипы с лиофилизированными реактивами позволяют избежать сложностей, связанных с хранением при низкой температуре, сокращают количество стадий пипетирования и тем самым уменьшают риск ложноположительных результатов, которые могут быть вызваны контаминацией проб. В работе предложен метод иммобилизации реагентов для ПЦР в микрореакторах алюминиевых микрочипов, поверхность которых была предварительно пассивирована с помощью плазмохимического осаждения из газовой фазы (ПХО). В качестве модельных объектов для лиофилизации выбраны тест-системы для определения фрагмента гена 35S вируса мозаики цветной капусты, используемого в генетически-модифицированных организмах (ГМО), фрагмента геномной ДНК микроорганизма Mycoplasma hominis, а также фрагмента гена глобина человека (белковая часть гемоглобина человека). Разработана экспериментальная установка для лиофилизации, которая позволила оптимизировать условия проведения процесса. Показано, что полученные таким образом алюминиевые микрочипы могут храниться не менее двух месяцев при комнатной температуре, и их аналитические характеристики сравнимы с ПЦР с использованием жидких реактивов, при в 20 раз меньшем потреблении реактивов и в 2-3 раза меньшем количестве операции пипетирования.Microchip analytical systems for the polymerase chain reaction (PCR) have attracted an increased interest of researchers in recent years. Aluminum microchips are particularly promising for the microchip PCR techniques implementation in a routine laboratory practice. However, the surface of the chips needs to be chemically inert for the PCR inhibition prevention and improved analytical characteristics of the system. It should also be noted that the possibility of decreasing of handling procedures and operator mistakes by using lyophilized reagents inside microreactor remains very attractive. Such microchip systems allow avoiding some of the storage difficulties and significantly decrease the number of pipetting stages. All of these lead to minimizing the risk of false positive results due to the sample cross-contamination. In this paper a method of PCR reagents lyophilization into the microreactors of aluminum microchips was developed. The surface of the microchip was modified using the Plasma Enhanced Chemical Vapor Deposition (PE CVD). As the model reagents for the lyophilization, the PCR test systems for 35S gene fragment determination (cauliflower mosaic virus) and human globin (protein part of human GEM) were used. The newly developed experimental system for lyophilization allowed optimizing the process for several microchips. It was shown that the obtained aluminum microchip could be stored under the ambient conditions up to 5 months and their analytical characteristics are comparable with the test-tube PCR, with 20 times less the reagents consumption and 2-3 times less the number of pipetting steps

Antibiofilm activity of aminopropanol derivatives against Pseudomonas aeruginosa

Bacterial biofilm, particularly formed by Pseudomonas aeruginosa, are a cause of severe chronic infectious diseases. Bacteria within a biofilm are phenotypically more resistant to antibiotics and the macroorganism immune system, making it an important virulence factor for many microbes. The aminopropanol derivatives with adamantyl (KVM-97) and N-alkylaryl radicals (KVM-194, KVM-204, KVM-261, and KVM-262) were used as study object. The aim of this study was to investigate the antibiofilm activity of compounds on biofilm formation and on mature biofilm of P. aeruginosa. The effects of the aminopropanol derivatives on the biofilm mass were evaluated by using crystal violet assay. Ciprofloxacin, meropenem, ceftazidime, gentamicin were used as reference substances. Reported results demonstrate that all compounds displayed antibiofilm activity at the tested concentrations. Remarkable reduction in biofilm formation of P. aeruginosa was found after treatment with KVM-97, KVM-261 and KVM-262 in high concentration (5× MIC), biofilm inhibition activity were 84.3%, 90.5% and 83.3% respectively. After a treatment with KVM-204 at 250 μg/ml (5× MIC) 76.6% of the preformed 24-hr biofilms were destroyed. Furthermore, compounds KVM-97, KVM-194, and KVM-261 in both concentrations showed potent antibiofilm activity against the P. aeruginosa, inhibition activity values being between 56.7 and 65.7%. All tested compounds in dose-dependent manner exhibited pronounced inhibition activity against mature 5-days P. аeruginosa biofilm. It was also observed that tested compounds show high antibiofilm activity in comparison to reference antimicrobials. The aminopropanol derivatives may provide templates for a new group of antimicrobial agents and potential future therapeutics for treating chronic infections

Antiseptic properties of aminoadamantane derivative

Development of microbial resistance to current antimicrobial drugs created a critical need of the new antiseptics. The object of our study was phenyladamantane derivative (4-(adamantyl-1)-1-(1-aminobutyl)benzol, AM-166). The aim of the presented study was to investigate the specific activity of 4% AM-166 solution in isopropanol and 5% AM-166 solution in 76% ethanol (manufactured by PJSC SIC «Borshchahivskiy CPP») against the wide spectrum of bacteria and fungi, and effective concentrations and exposition time determination. Desinfectant/antiseptic activity was evaluated by quantitative suspension method with subsequent neutralization. Our results showed that both solutions exhibited similar activity against gram-positive and gram-negative bacteria as well as against yeasts. Antibacterial and antifungal action was demonstrated for all investigated concentrations (initial solution, 5-fold and 10-fold dilutions), observed effect was maintained throughout the whole observation period (from 5 to 30 min). Tested solutions in initial concentrations demonstrated fungicidal activity against A. niger. 5-fold dilution of 5% AM-166 solution in 76% ethanol was more effective than 5-fold dilution of 4% AM-166 solution in isopropanol. 10-fold dilutions of both solutions were ineffective against A. niger. The data obtained suggest the prospects of adamantane derivatives for the development of novel antiseptics

Анализ структуры бактериемий и чувствительности к антибиотикам микроорганизмов, выделенных в отделениях реанимации и интенсивной терапии в скоропомощном стационаре в период с 2003 по 2021 г.: ретроспективное наблюдательное исследование

АКТУАЛЬНОСТЬ: Создание протоколов эффективной эмпирической антимикробной терапии невозможно без анализа актуальных данных чувствительности к антибиотикам. ЦЕЛЬ ИССЛЕДОВАНИЯ: Анализ изменения этиологической значимости ведущих возбудителей бактериемий в отделении реанимации и интенсивной терапии (ОРИТ) в период с 2003 по 2021 г. и их чувствительности к антибиотикам; изучение типов карбапенемаз у наиболее актуальных возбудителей — Klebsiella pneumoniae и Acinetobacter baumannii. МАТЕРИАЛЫ И МЕТОДЫ: В анализ были включены все клинически значимые микроорганизмы, выделенные из крови пациентов, находившихся ОРИТ общего профиля, рассчитанном на 12 коек, в скоропомощном стационаре г. Москвы в период с 2003 по 2021 г. Идентификацию микроорганизмов и определение чувствительности к антибиотикам проводили в автоматическом анализаторе PHOENIX и стандартизированным диско-диффузионным методом. Детекцию карбапенемаз осуществляли методом полимеразной цепной реакции. РЕЗУЛЬТАТЫ: Исследовано 17 034 образца крови, из которых было выделено 6372 микроорганизма. В указанный период доля грамотрицательных микроорганизмов увеличилась с 21,0 до 52,8 %, преимущественно за счет K. pneumoniae (1,0–24,5 %) и A. baumannii (0–9,1 %). Устойчивость этих микроорганизмов к карбапенемам с 2008 по 2021 г. значительно увеличилась: K. pneumoniae — с 2,3 до 70,3 %, A. baumannii — с 7,5 до 99,5 %. Штаммы K. pneumoniae продуцировали следующие типы карбапенемаз: OXA-48 (73,8 %), KPC (6,2 %), NDM (1,5 %), NDM + OXA-48 (15,4 %), KPC + OXA-48 (3,1 %). Все штаммы A. baumannii продуцировали карбапенемазы OXA-40. Продуценты карбапенемазы OXA-48 были устойчивы к колистину и тигециклину в 14,6 и 44,8 % случаев соответственно, к цефтазидиму/авибактаму — в 4,2 % случаев. ВЫВОДЫ: Увеличение уровня антимикробной резистентности в последние годы среди доминирующих в бактериемии грамотрицательных микроорганизмов является очень значимой проблемой в ОРИТ. Большинство штаммов K. pneumoniae характеризуются множественной резистентностью и около 10 % — экстремальной, или панрезистентностью. Практически все штаммы A. baumannii относятся к категории экстремально резистентных

Higgs Bosons and the Indirect Search for WIMPs

We investigated the contribution of the MSSM Higgs bosons produced in the neutralino annihilation in the Earth and Sun to the total WIMPs detection signals. We found that this contribution is very important and results in a lower bound for the muon flux from the Sun of 10^{-7} - 10^{-8} m^{-2} yr^{-1} for neutralinos heavier than 200 GeV. We noticed that due to the SUSY charged Higgs bosons one can expect an energetic tau neutrino flux from the Sun at a level of 10^2 m^{-2} yr^{-1}.Comment: 10 pages, 4 figures. Talk given at Dark2000, Heidelberg, Germany, 10-15 July, 200