Strategic guidelines of the educational interactive environment as a basis to develop the axiosphere of a future teacher

The article examines the phenomenon of the interactive educational environment as a carefully constructed context to develop the future teacher’s personality as a result of organized human productive activity where the set of relations and connections subjectively specifies that are specific conditions to develop the future teacher’s axiosphere. The relevance of this work is to explore the dialogue educational environment as a factor forming the future teacher's values, which corresponds to modern trends of development in higher pedagogical education: humanizing and using dialogues in the educational environment; purposeful intellectual and moral development of the future professional in the context of its inclusion in a variety of independent activities, individual routes of subjects in educational process; the motivation development to pedagogical activity and professional students’ education; the personality to universal human and national values; the use in educational process of higher school and interactive technologies of education, pedagogical innovations, etc. Appealing to the analysis of the interactive educational environment has allowed identifying and characterizing components of his conceptual model: motivational-target, informationalcommunicative, subject to subject, content-axiological, organizational-active and productive. A role of principles of dialogic education is shown as: the principle of information enrichment, the principle of unity in diversity, freedom of choice, the dynamic principle, the identity principle, the principle of emotional openness. The authors present the strategic guidelines of the interactive environment education: the development of social cooperation, using the pedagogical peaceful potential, the development of interpersonal and intergroup relations, organizing children’s education as a strategic national priority. The resource analysis of dialogue area in education led the authors to the definition of ways developing the future teacher’s axiosphere, the formation of their socio-cultural, professionalpedagogical and personal values. Among the modern technologies there is dedicated interactive educational technology: a dialogue, educational discussion, a communication technology, technology of critical thinking development, technology of pedagogical workshops, moral dilemmas technology (L. Colberg). Key words: dialogue environment education, dialogue, axiosphere of a future teacher, sociocultural, professional-pedagogical and personal values of the teachers, the subjects of education, value-semantic interaction, modern teache

Cultivar features of polyphenolic compounds and ascorbic acid accumulation in the cherry fruits (Prunus cerasus L.) in the Southern Steppe of Ukraine

Received: June 1st, 2022 ; Accepted: September 26th, 2022 ; Published: October 14th, 2022 ; Correspondence: [email protected] is a popular and widespread fruit crop in many European countries. Significant areas of its plantation are located in the Southern Steppe subzone of Ukraine. Modern biochemical research aims to determine the amplitude of cherries varietal difference within the studied species and determine selection possibilities for the most important chemical components. In this regard, the study of fruits biochemical composition of different cherries cultivars is relevant. The aim of the research was to build a mathematical model based on Multiple linear regression method, which reveals the degree of weather factors influence on the dynamics of polyphenolic compounds and ascorbic acid accumulation in cherries fruits in the Southern Steppe subzone of Ukraine and in regions with similar hydrothermal parameters. The cultivar ‘Ihrushka’ was characterized by the lowest variability in the concentration of polyphenolic compounds with the value of the variation coefficient of 9.9%. The optimal average concentration of polyphenolic compounds at the level of 224.6 mg 100 g-1 had fruits of the cherry cultivar ‘Siianets Turovtsevoi’ (Vp – 12.8%). Fruits of the ‘Vstrecha’ cultivar were characterized by the optimal average concentration of ascorbic acid at the level of 9.6 mg 100 g-1 and variability of the indicator 14.0%. The dominant influence of varietal characteristics on the polyphenolic compounds accumulation in cherry fruits has been established. The share of the factor impact B was 41.3%. It was determined that weather conditions with a share of influence of 69.2% are crucial for the formation of the ascorbic acid. The correlation analysis showed the presence of a linear correlation between seven weather factors (Хі, i = 1..7) and the concentration of polyphenolic compounds (Y1) and ascorbic acid (Y2) in cherry fruits. The values of the pairwise correlation coefficients rY1Xi , rY2Xi i = 1. .7 were within the interval [-0.55; 0.55], which showed the presence of an impact between these weather factors and the studied indicators. The average monthly precipitation in June became decisive for the accumulation of polypolyphenolic compounds (∆X2 = 35.2%). The average monthly amount of precipitation in May was determined to be the most important for the formation of the ascorbic acid level (∆X1 = 37.1%)

Structure of the Nucleotide Radical Formed during Reaction of CDP/TTP with the E441Q-α2β2 of E. coli Ribonucleotide Reductase

The Escherichia coli ribonucleotide reductase (RNR) catalyzes the conversion of nucleoside diphosphates to deoxynucleotides and requires a diferric-tyrosyl radical cofactor for catalysis. RNR is composed of a 1:1 complex of two homodimeric subunits: α and β. Incubation of the E441Q-α mutant RNR with substrate CDP and allosteric effector TTP results in loss of the tyrosyl radical and formation of two new radicals on the 200 ms to min time scale. The first radical was previously established by stopped flow UV/vis spectroscopy and pulsed high field EPR spectroscopy to be a disulfide radical anion. The second radical was proposed to be a 4′-radical of a 3′-keto-2′-deoxycytidine 5′-diphosphate. To identify the structure of the nucleotide radical [1′-[superscript 2]H], [2′-[superscript 2]H], [4′-[superscript 2]H], [5′-[superscript 2]H], [U−[superscript 13]C, [superscript 15]N], [U−[superscript 15]N], and [5,6 -[superscript 2]H] CDP and [β-[superscript 2]H] cysteine-α were synthesized and incubated with E441Q-α2β2 and TTP. The nucleotide radical was examined by 9 GHz and 140 GHz pulsed EPR spectroscopy and 35 GHz ENDOR spectroscopy. Substitution of [superscript 2]H at C4′ and C1′ altered the observed hyperfine interactions of the nucleotide radical and established that the observed structure was not that predicted. DFT calculations (B3LYP/IGLO-III/B3LYP/TZVP) were carried out in an effort to recapitulate the spectroscopic observations and lead to a new structure consistent with all of the experimental data. The results indicate, unexpectedly, that the radical is a semidione nucleotide radical of cytidine 5′-diphosphate. The relationship of this radical to the disulfide radical anion is discussed.National Institutes of Health (U.S.) (GM29595)(EB002804)(EB002026

Analysis of gas exchange in seedlings of Acer saccharum : integration of field and laboratory studies

In the field, photosynthesis of Acer saccharum seedlings was rarely light saturated, even though light saturation occurs at about 100 μmol quanta m -2 s -1 photosynthetic photon flux density (PPFD). PPFD during more than 75% of the daylight period was 50 μmol m -2 s -1 or less. At these low PPFD's there is a marked interaction of PPFD with the initial slope (CE) of the CO 2 response. At PPFD-saturation CE was 0.018 μmol m -2 s -1 /(μl/l). The apparent quantum efficiency (incident PPFD) at saturating CO 2 was 0.05–0.08 mol/mol. and PPFD-saturated CO 2 exchange was 6–8 μmol m -2 s -1 . The ratio of internal CO 2 concentration to external ( C i / C a ) was 0.7 to 0.8 except during sunflecks when it decreased to 0.5. The decrease in C i / C a during sunflecks was the result of the slow response of stomates to increased PPFD compared to the response of net photosynthesis. An empirical model, which included the above parameters was used to simulate the measured CO 2 exchange rate for portions of two days. Parameter values for the model were determined in experiments separate from the daily time courses being sumulated. Analysis of the field data, partly through the use of simulations, indicate that the elimination of sunflecks would reduce net carbon gain by 5–10%.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47755/1/442_2004_Article_BF00378907.pd