393 research outputs found
Derivation of Bearing Capacity Equation for a Two Layered System of Weak Clay Layer Overlaid by Dense Sand Layer
Calculation of the ultimate bearing capacity of shallow footing on a two layered
system of soil depends on the pattern of the failure surface that develops below
the footing. For a weak clay layer overlaid by a top dense sand layer, previous
studies assumed that the failure surface is a punching shear failure through the
upper sand layer and Prandtl's failure mode in the bottom weak clay layer. By
adapting this assumption in this study, the ultimate bearing capacity equation
was derived as a function of the properties of soils, the footing width, and the
topsoil thickness. The paper presents a detailed parametric study of the design
parameters including the effect of angle of friction, the ratio of the thickness
of sand layer to the footing width, the ratio of the depth of embedment to the
footing width, and the ratio of the clay soil cohesion to the product of the clay
unit weight by the footing width. Design charts were developed in dimensionless
form for very wide ranges of design parameters. The available method based on
the limit equalibrium analysis was developed in dimensionlised form and for a
limited range of design parametrs. The new charts give another option for
those who believe that the design charts developed based on the upper limit
analysis overestimate the bearing capacity due to the very nature of the upper
bound solution. The new design charts are limited to shallow footings
Sequence Specific Motor Performance Gains after Memory Consolidation in Children and Adolescents
Memory consolidation for a trained sequence of finger opposition movements, in 9- and 12-year-old children, was recently found to be significantly less susceptible to interference by a subsequent training experience, compared to that of 17-year-olds. It was suggested that, in children, the experience of training on any sequence of finger movements may affect the performance of the sequence elements, component movements, rather than the sequence as a unit; the latter has been implicated in the learning of the task by adults. This hypothesis implied a possible childhood advantage in the ability to transfer the gains from a trained to the reversed, untrained, sequence of movements. Here we report the results of transfer tests undertaken to test this proposal in 9-, 12-, and 17-year-olds after training in the finger-to-thumb opposition sequence (FOS) learning task. Our results show that the performance gains in the trained sequence partially transferred from the left, trained hand, to the untrained hand at 48-hours after a single training session in the three age-groups tested. However, there was very little transfer of the gains from the trained to the untrained, reversed, sequence performed by either hand. The results indicate sequence specific post-training gains in FOS performance, as opposed to a general improvement in performance of the individual, component, movements that comprised both the trained and untrained sequences. These results do not support the proposal that the reduced susceptibility to interference, in children before adolescence, reflects a difference in movement syntax representation after training
Iron oxide nanoparticles for neuronal cell applications: uptake study and magnetic manipulations.
BACKGROUND: The ability to direct and manipulate neuronal cells has important potential in therapeutics and neural network studies. An emerging approach for remotely guiding cells is by incorporating magnetic nanoparticles (MNPs) into cells and transferring the cells into magnetic sensitive units. Recent developments offer exciting possibilities of magnetic manipulations of MNPs-loaded cells by external magnetic fields. In the present study, we evaluated and characterized uptake properties for optimal loading of cells by MNPs. We examined the interactions between MNPs of different cores and coatings, with primary neurons and neuron-like cells. RESULTS: We found that uncoated-maghemite iron oxide nanoparticles maximally interact and penetrate into cells with no cytotoxic effect. We observed that the cellular uptake of the MNPs depends on the time of incubation and the concentration of nanoparticles in the medium. The morphology patterns of the neuronal cells were not affected by MNPs uptake and neurons remained electrically active. We theoretically modeled magnetic fluxes and demonstrated experimentally the response of MNP-loaded cells to the magnetic fields affecting cell motility. Furthermore, we successfully directed neurite growth orientation along regeneration. CONCLUSIONS: Applying mechanical forces via magnetic mediators is a useful approach for biomedical applications. We have examined several types of MNPs and studied the uptake behavior optimized for magnetic neuronal manipulations.MM gratefully acknowledges the BINA Scholarship for Outstanding Graduate Students. The authors thank Michelle Katz for her help with the morphology measurements
User-centered design of the C3-cloud platform for elderly with multiple diseases - functional requirements and application testing
The number of patients with multimorbidity has been steadily increasing in the modern aging societies. The European C3-Cloud project provides a multidisciplinary and patient-centered “Collaborative Care and Cure-system” for the management of elderly with multimorbidity, enabling continuous coordination of care activities between multidisciplinary care teams (MDTs), patients and informal caregivers (ICG). In this study various components of the infrastructure were tested to fulfill the functional requirements and the entire system was subjected to an early application testing involving different groups of end-users. MDTs from participating European regions were involved in requirement elicitation and test formulation, resulting in 57 questions, distributed via an internet platform to 48 test participants (22 MDTs, 26 patients) from three pilot sites. The results indicate a high level of satisfaction with all components. Early testing also provided feedback for technical improvement of the entire system, and the paper points out useful evaluation methods
Interlayer Registry Determines the Sliding Potential of Layered Metal Dichalcogenides: The case of 2H-MoS2
We provide a simple and intuitive explanation for the interlayer sliding
energy landscape of metal dichalcogenides. Based on the recently introduced
registry index (RI) concept, we define a purely geometrical parameter which
quantifies the degree of interlayer commensurability in the layered phase of
molybdenum disulphide (2HMoS2). A direct relation between the sliding energy
landscape and the corresponding interlayer registry surface of 2H-MoS2 is
discovered thus marking the registry index as a computationally efficient means
for studying the tribology of complex nanoscale material interfaces in the
wearless friction regime.Comment: 13 pages, 7 figure
Graphene transistors are insensitive to pH changes in solution
We observe very small gate-voltage shifts in the transfer characteristic of
as-prepared graphene field-effect transistors (GFETs) when the pH of the buffer
is changed. This observation is in strong contrast to Si-based ion-sensitive
FETs. The low gate-shift of a GFET can be further reduced if the graphene
surface is covered with a hydrophobic fluorobenzene layer. If a thin Al-oxide
layer is applied instead, the opposite happens. This suggests that clean
graphene does not sense the chemical potential of protons. A GFET can therefore
be used as a reference electrode in an aqueous electrolyte. Our finding sheds
light on the large variety of pH-induced gate shifts that have been published
for GFETs in the recent literature
Insulin-Producing Cells Generated from Dedifferentiated Human Pancreatic Beta Cells Expanded In Vitro
Expansion of beta cells from the limited number of adult human islet donors is an attractive prospect for increasing cell availability for cell therapy of diabetes. However, attempts at expanding human islet cells in tissue culture result in loss of beta-cell phenotype. Using a lineage-tracing approach we provided evidence for massive proliferation of beta-cell-derived (BCD) cells within these cultures. Expansion involves dedifferentiation resembling epithelial-mesenchymal transition (EMT). Epigenetic analyses indicate that key beta-cell genes maintain open chromatin structure in expanded BCD cells, although they are not transcribed. Here we investigated whether BCD cells can be redifferentiated into beta-like cells.Redifferentiation conditions were screened by following activation of an insulin-DsRed2 reporter gene. Redifferentiated cells were characterized for gene expression, insulin content and secretion assays, and presence of secretory vesicles by electron microscopy. BCD cells were induced to redifferentiate by a combination of soluble factors. The redifferentiated cells expressed beta-cell genes, stored insulin in typical secretory vesicles, and released it in response to glucose. The redifferentiation process involved mesenchymal-epithelial transition, as judged by changes in gene expression. Moreover, inhibition of the EMT effector SLUG (SNAI2) using shRNA resulted in stimulation of redifferentiation. Lineage-traced cells also gave rise at a low rate to cells expressing other islet hormones, suggesting transition of BCD cells through an islet progenitor-like stage during redifferentiation.These findings demonstrate for the first time that expanded dedifferentiated beta cells can be induced to redifferentiate in culture. The findings suggest that ex-vivo expansion of adult human islet cells is a promising approach for generation of insulin-producing cells for transplantation, as well as basic research, toxicology studies, and drug screening
Localisation, personalisation and delivery of best practice guidelines on an integrated care and cure cloud architecture : the C3-cloud approach to managing multimorbidity
Background: C3-Cloud is an integrated care ICT infrastructure offering seamless patient-centered approach to managing multimorbidity, deployed in three European pilot sites. Challenge: The digital delivery of best practice guidelines unified for multimorbidity, customized to local practice, offering the capability to improve patient personalization and benefit. Method: C3-Cloud has adopted a co-production approach to developing unified multimorbidity guidelines, by collating and reconciling best practice guidelines for each condition. Clinical and technical teams at pilot sites and the C3-Cloud consortium worked in tandem to create the specification and technical implementation. Results: C3-Cloud offers CDSS for diabetes, renal failure, depression and congenital heart failure, with over 300 rules and checks that deliver four best practice guidelines in parallel, customized for each pilot site. Conclusions: The process provided a traceable, maintainable and audited digitally delivered collated and reconciled guidelines
Electromechanical properties of suspended Graphene Nanoribbons
Graphene nanoribbons present diverse electronic properties ranging from
semiconducting to half-metallic, depending on their geometry, dimensions and
chemical composition. Here we present a route to control these properties via
externally applied mechanical deformations. Using state-of-the-art density
functional theory calculations combined with classical elasticity theory
considerations, we find a remarkable Young's modulus value of ~7 TPa for
ultra-narrow graphene strips and a pronounced electromechanical response
towards bending and torsional deformations. Given the current advances in the
synthesis of nanoscale graphene derivatives, our predictions can be
experimentally verified opening the way to the design and fabrication of
miniature electromechanical sensors and devices based on ultra-narrow graphene
nanoribbons.Comment: 12 pages, 6 figure
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