Conditions for collective action: understanding factors supporting and constraining community-based fish culture in Bangladesh, Cambodia and Vietnam

In 2005, the WorldFish Center embarked on a project to pilot test approaches to community-based fish culture (CBFC) in five countries. A previous study conducted between 1997-2000 demonstrated the potential of the approach in Bangladesh and Vietnam, although a greater understanding was needed regarding the social and institutional factors that would permit the development of CBFC in larger waterbodies to reach a greater number of beneficiaries. The five countries selected for dissemination of CBFC included Cambodia, Vietnam, China, Bangladesh and Mali, each very different in terms of history, politics, social-cultural context, aquaculture experience and development status. They appeared to share environmental characteristics, all having seasonally flooding areas and experience of rice-fish culture. This report presents the findings of this study, based on a detailed evaluation undertaken in 2008-2009 in Cambodia, Vietnam and Bangladesh. Mali and China were not included in the study, both for reasons of time and cost, and due to the different path that project development had taken in each country. Although the research was conducted as consistently as possible across the three countries, using the same methodology in each location, the results are nonetheless also indicative of the differences encountered at each location. The diversity of reasons why CBFC worked and didnÆt work led to difficulties in drawing conclusions across countries, or in quantifying results, with the exception of Vietnam where the number of communities involved in the study made quantification possible. The findings of the study are therefore primarily qualitative in nature, with figures provided relating to number of responses where available. The issues raised by respondents participating in the study are grouped according to environmental conditions, socio-cultural conditions, livelihood context, institutional context, markets and economic viability, technical issues and implementation and incentives and disincentives for uptake and continuance. The report concludes with a summary of lessons learned.Inland fisheries, Flood plains, Rice field aquaculture, Participatory approach, Cambodia, Vietnam, Bangladesh,

Contextual analysis in two villages of the Niger River Inner Delta

The Community-based Fish Culture in Seasonal Floodplains and Irrigation Systems (CBFC) project is a five year research project supported by the Challenge Program on Water and Food (CPWF), with the aim of increasing productivity of seasonally occurring water bodies through aquaculture. The project has been implemented in Bangladesh, Cambodia, China, Mali and Vietnam, where technical and institutional options for community based aquaculture have been tested. The project began in 2005 and was completed in March 2010. The objective of the study was to improve our knowledge of mare management and the livelihoods of two villages of the Niger Inner Delta, Severi and Komio. We wanted to understand mare access and fishery management and to highlight the different stakeholders involved in mare management as well as the different rules regulating access to the various mare resources. We implemented a series of Focus Group Discussion with the different socio-economic groups of the villages (fisherfolk, farmer-fishers, and herders) and a series of semi-structured interviews with key informants and stakeholders involved in mare management took place in July 2008.Fishing rights, River fisheries, Livelihoods

Aquaculture for the poor in Cambodia

This lesson learned reviewed the current status of aquaculture in Cambodia. It primarily covers inland fish farming development and coastal aquaculture projects targeted at poverty alleviation and food security. It focuses on approaches aimed at developing low cost systems, and less on high input aquaculture systems that are usually inaccessible to poor families.

Institutional histories, seasonal floodplains (mares), and livelihood impacts of fish stocking in the Inner Niger River Delta of Mali

The Community-based Fish Culture in Seasonal Floodplains and Irrigation Systems (CBFC) project is a five year research project supported by the Challenge Program on Water and Food (CPWF), with the aim of increasing productivity of seasonally occurring water bodies through aquaculture. The project has been implemented in Bangladesh, Cambodia, China, Mali and Vietnam, where technical and institutional options for community based aquaculture have been tested. The project began in 2005 and was completed in March 2010. The seasonally flooded depressions in the Inner Niger Delta (known as mares) represent a critical fishery resource for the inhabitants of the village of Komio, and at present, access is open to all residents. A proposal to build stocked fish enclosures in the main village mare presents potential benefits and risks. On one hand, overall productivity in the mare could be significantly increased, providing important sources of protein and cash during the annual drought period, when few livelihood activities can be performed and when village livelihoods are at their most vulnerable. Enhanced productivity in mares may also decrease local household pressures for seasonal labor migration. On the other hand, a resulting increase in the value of these mares may encourage elite capture of project benefits or rentseeking by certain village leaders of the landowning Marka ethnic group. Using qualitative interviews and focus group discussions, the study provides evidence of how local institutional and leadership capacity for equitable common property resource management have evolved since the introduction of irrigated farming systems (known as PΘrimΦtres IrriguΘs Villageois or PIVs) in the 1990s.Fishing rights, River fisheries, Livelihoods

Accounting Problems Under the Excess Profits Tax

DNA vaccines based on subunits from pathogens have several advantages over other vaccine strategies. DNA vaccines can easily be modified, they show good safety profiles, are stable and inexpensive to produce, and the immune response can be focused to the antigen of interest. However, the immunogenicity of DNA vaccines which is generally quite low needs to be improved. Electroporation and co-delivery of genetically encoded immune adjuvants are two strategies aiming at increasing the efficacy of DNA vaccines. Here, we have examined whether targeting to antigen-presenting cells (APC) could increase the immune response to surface envelope glycoprotein (Env) gp120 from Human Immunodeficiency Virus type 1 (HIV- 1). To target APC, we utilized a homodimeric vaccine format denoted vaccibody, which enables covalent fusion of gp120 to molecules that can target APC. Two molecules were tested for their efficiency as targeting units: the antibody-derived single chain Fragment variable (scFv) specific for the major histocompatilibility complex (MHC) class II I-E molecules, and the CC chemokine ligand 3 (CCL3). The vaccines were delivered as DNA into muscle of mice with or without electroporation. Targeting of gp120 to MHC class II molecules induced antibodies that neutralized HIV-1 and that persisted for more than a year after one single immunization with electroporation. Targeting by CCL3 significantly increased the number of HIV-1 gp120-reactive CD8(+) T cells compared to non-targeted vaccines and gp120 delivered alone in the absence of electroporation. The data suggest that chemokines are promising molecular adjuvants because small amounts can attract immune cells and promote immune responses without advanced equipment such as electroporation.Funding Agencies|Research Council of Norway; Odd Fellow</p

Tear fluid biomarkers in ocular and systemic disease: potential use for predictive, preventive and personalised medicine

In the field of predictive, preventive and personalised medicine, researchers are keen to identify novel and reliable ways to predict and diagnose disease, as well as to monitor patient response to therapeutic agents. In the last decade alone, the sensitivity of profiling technologies has undergone huge improvements in detection sensitivity, thus allowing quantification of minute samples, for example body fluids that were previously difficult to assay. As a consequence, there has been a huge increase in tear fluid investigation, predominantly in the field of ocular surface disease. As tears are a more accessible and less complex body fluid (than serum or plasma) and sampling is much less invasive, research is starting to focus on how disease processes affect the proteomic, lipidomic and metabolomic composition of the tear film. By determining compositional changes to tear profiles, crucial pathways in disease progression may be identified, allowing for more predictive and personalised therapy of the individual. This article will provide an overview of the various putative tear fluid biomarkers that have been identified to date, ranging from ocular surface disease and retinopathies to cancer and multiple sclerosis. Putative tear fluid biomarkers of ocular disorders, as well as the more recent field of systemic disease biomarkers, will be shown

Cytolytic DNA vaccine encoding lytic perforin augments the maturation of- and antigen presentation by- dendritic cells in a time-dependent manner

The use of cost-effective vaccines capable of inducing robust CD8+ T cell immunity will contribute significantly towards the elimination of persistent viral infections and cancers worldwide. We have previously reported that a cytolytic DNA vaccine encoding an immunogen and a truncated mouse perforin (PRF) protein significantly augments anti-viral T cell (including CD8+ T cell) immunity. Thus, the current study investigated whether this vaccine enhances activation of dendritic cells (DCs) resulting in greater priming of CD8+ T cell immunity. In vitro data showed that transfection of HEK293T cells with the cytolytic DNA resulted in the release of lactate dehydrogenase, indicative of necrotic/lytic cell death. In vitro exposure of this lytic cell debris to purified DCs from naïve C57BL/6 mice resulted in maturation of DCs as determined by up-regulation of CD80/CD86. Using activation/proliferation of adoptively transferred OT-I CD8+ T cells to measure antigen presentation by DCs in vivo, it was determined that cytolytic DNA immunisation resulted in a time-dependent increase in the proliferation of OT-I CD8+ T cells compared to canonical DNA immunisation. Overall, the data suggest that the cytolytic DNA vaccine increases the activity of DCs which has important implications for the design of DNA vaccines to improve their translational prospects.Danushka K. Wijesundara, Wenbo Yu, Ben J. C. Quah, Preethi Eldi, John D. Hayball, Kerrilyn R. Diener, Ilia Voskoboinik, Eric J. Gowans, and Branka Grubor-Bau