Molecular diversification and preliminary evaluations of some satsuma selections’ performance under mediterranean conditions

Satsuma (Citrus unshiu Marc.) has been cultivated for a long time in Turkey, and therefore variations for agronomical traits are likely among cultivated satsumas due to bud mutations. The objectives of this study were to determine variations for some selected agronomical traits and genetic markers among 21 new satsumas derived from selections. Fruit yield, fruit quality and molecular diversification of these clones were determined. The clones of 62 Adana, 4/2 Izmir and 11/1 Izmir indicated the highest yield. The fruits obtained from all clones were heavier than the control (Owari Satsuma). Molecular analysis, as assessed with 9 random amplification of polymorphic DNA (RAPD) and 14 simple sequence repeats (SSR) primers, indicated that satsuma clones showed a narrow genetic base suggesting that the observed morphological polymorphism within the group must be associated with  somatic mutations which were not detected by these molecular markers.Key words: Mandarins, clonal selection, citrus

The general and volatile properties and the quality of two newly selected satsuma clones (11/1 İzmir and 30/ İzmir) grown under mediterranean ecological conditions

The general and volatile properties, and the quality of two new Satsuma clones – 11/1 İzmir and 30/İzmir – selected under a Citrus Bud Wood Selection Program, were compared with Owari Satsuma samples grown under Adana ecological conditions. Gas chromatography, mass spectrometry, flame ionization detection (GC/MS/FID) analysis was used in the identification and quantification of the aromatic compounds, and a sensory profile analysis was performed to complete the general understanding using chemical analysis. The general analysis showed that the clone samples have higher yields, are more intense in skin color and more acidic; however, the total soluble solids and total sugar amount are lower than the Owari Satsuma samples. The terpenes are the major aromatic compound class. Along with other terpenes, there were significant amounts of dl-limonene followed by ?-terpinene, ß-elemene, linalool and ?-terpineol. The sensory analysis showed that the Owari Satsuma samples are sweeter and riper, with a better floral, spicy-citrus flavor than both clones. Both clones had a lower “Overall liking” rating than the Owari Satsuma samples. © 2019, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved

Molecular characterization of sour orange (citrus aurantium) accessions and their relatives using SSR and SRAP markers

Citrus production with its many varieties is of importance since it provides economically important products for Turkish exports. Sour orange is a rootstock commonly used for propagating the different scion varieties. Knowledge of the genetic diversity of the rootstock accessions would be useful in order to improve citrus breeding programs. We studied genetic relationships and diversity of 51 accessions of sour orange (Citrus aurantium) and their relatives using SSR (simple sequence repeat) and SRAP (sequence-related amplifed polymorphism) molecular markers. Twenty-one SRAP primer combinations were tested on these accessions and relatives, producing 167 polymorphic fragments, with a mean of 8.0 and a mean polymorphism information content value of 0.47. Seventeen SSR primers also produced 30 polymorphic fragments, with a mean of 1.4 per primer and a mean polymorphism information content value of 0.39. The unweighted pair-group method with arithmetic average analysis using combined SSR and SRAP data showed a similarity range from 0.71 to 1.00 among the accessions. In the cluster analysis, sour orange relatives were indicated as a separate group from sour orange. 'Macrophylla' and 'Mexican lime' were the accessions most distinct (0.71) from the others. We conclude that genetic diversity in these sour orange accessions is lower and some of them were identical. © FUNPEC-RP www.funpecrp.com.br

Fruit yield and quality of santa teresa lemon on seven rootstocks in Adana (Turkey)

The effects of various rootstocks on yield, yield efficiency, vegetative growth and fruit quality of Santa Teresa lemon variety were evaluated under Adana ecological conditions during 2004 and 2005. Canopy volume was the lowest for trees on Carrizo citrange, followed by Troyer citrange and Citrus ampullacea. Total cumulative yield over two years of production was highest on C. obovoidea, followed by that on Citrus sulcata. On the other hand, the trees on C. citrange were the most efficient in yield per cubic meter of canopy volume (CV) and trunk cross-sectional area (TCSA) due to its small TCSA and CV. The largest fruit size was obtained from the trees on C. sulcata, Taiwanica, C. ampullacea, followed by sour orange and Citrus obovoidea. The highest total acid content of fruits was found with sour orange and T. citrange, followed by C. obovoidea. When all the results are considered, it can be concluded that C. obovoidea was more promising rootstock for Santa Teresa lemon in Çukurova Region. © 2010 Academic Journals