The current state of the problem of soft tissue defects replacement in the lower extremities in conditions of chronic osteomyelitis

Based on the data of scientific literature, the current state of the problem of soft tissue defects replacement in the lower extremities in conditions of chronic osteomyelitis has been analyzed. Taking into account certain requirements for the replacement of defects of this nature, the following reconstructive tasks have been indicated. First of all, for adequate restoration of the skin with simultaneous relief of the purulent-necrotic process, it is necessary to use blood-supplied tissue complexes, which, if possible, should be formed from tissues similar to those surrounding the defect. The predominant use of technically and economically beneficial interventions is of great importance. The most suitable are loco-regional flaps with axial blood supply, among which the most promising are perforant flaps. It is necessary to develop an algorithm for defects reconstruction using loco-regional perforant flaps, depending on the defect area. It is also necessary to conduct a comparative analysis of the anatomical advantages and effectiveness of using various perforant and insular flaps on the vascular pedicle. A one-stage orthoplastic approach will allow the most effective use of the achievements of reconstructive plastic surgery in orthopedics to restore limb function, which will improve the results of treatment of patients

Near-Infrared Fluorescent Proteins and Their Applications

High transparency, low light-scattering, and low autofluorescence of mammalian tissues in the near-infrared (NIR) spectral range (650-900 nm) open a possibility for in vivo imaging of biological processes at the micro-and macroscales to address basic and applied problems in biology and biomedicine. Recently, probes that absorb and fluoresce in the NIR optical range have been engineered using bacterial phytochromes-natural NIR light-absorbing photoreceptors that regulate metabolism in bacteria. Since the chromophore in all these proteins is biliverdin, a natural product of heme catabolism in mammalian cells, they can be used as genetically encoded fluorescent probes, similarly to GFP-like fluorescent proteins. In this review, we discuss photophysical and biochemical properties of NIR fluorescent proteins, reporters, and biosensors and analyze their characteristics required for expression of these molecules in mammalian cells. Structural features and molecular engineering of NIR fluorescent probes are discussed. Applications of NIR fluorescent proteins and biosensors for studies of molecular processes in cells, as well as for tissue and organ visualization in whole-body imaging in vivo, are described. We specifically focus on the use of NIR fluorescent probes in advanced imaging technologies that combine fluorescence and bioluminescence methods with photoacoustic tomography.Peer reviewe

Results of a search for 2β\beta-decay of 136^{136}Xe with high-pressure copper proportional counters in Baksan Neutrino Observatory

The experiment for the 2$\beta$-decay of $^{136}$Xe search with two high-pressure copper proportional counters has been held in Baksan neutrino observatory. The search for the process is based on comparison of spectra measured with natural and enriched xenon. No evidence has been found for 2$\beta$(2$\nu$)- and 2$\beta$(0$\nu$)-decay. The decay half lifetime limit based on data measured during 8000 h is T$_{1/2}$$\geq8.5\cdot10^{21}$yr for 2$\nu$-mode and T$_{1/2}$$\geq3.1\cdot10^{23}$yr for 0$\nu$-mode (90%C.L.).Comment: 9 pages, 8 figures; talk at the NANP'05 Conference; submitted to Phys. At. Nuc

The central engine of GRB 130831A and the energy breakdown of a relativistic explosion

Gamma-ray bursts (GRBs) are the most luminous explosions in the universe, yet the nature and physical properties of their energy sources are far from understood. Very important clues, however, can be inferred by studying the afterglows of these events. We present optical and X-ray observations of GRB 130831A obtained by Swift, Chandra, Skynet, RATIR, Maidanak, ISON, NOT, LT and GTC. This burst shows a steep drop in the X-ray light-curve at $\simeq 10^5$ s after the trigger, with a power-law decay index of $\alpha \sim 6$. Such a rare behaviour cannot be explained by the standard forward shock (FS) model and indicates that the emission, up to the fast decay at $10^5$ s, must be of "internal origin", produced by a dissipation process within an ultrarelativistic outflow. We propose that the source of such an outflow, which must produce the X-ray flux for $\simeq 1$ day in the cosmological rest frame, is a newly born magnetar or black hole. After the drop, the faint X-ray afterglow continues with a much shallower decay. The optical emission, on the other hand, shows no break across the X-ray steep decrease, and the late-time decays of both the X-ray and optical are consistent. Using both the X-ray and optical data, we show that the emission after $\simeq 10^5$ s can be explained well by the FS model. We model our data to derive the kinetic energy of the ejecta and thus measure the efficiency of the central engine of a GRB with emission of internal origin visible for a long time. Furthermore, we break down the energy budget of this GRB into the prompt emission, the late internal dissipation, the kinetic energy of the relativistic ejecta, and compare it with the energy of the associated supernova, SN 2013fu.Comment: Accepted for publication by MNRAS. 21 pages, 3 figures, 8 tables. Extra table with magnitudes in the sourc

La institucionalización de los principios de creación de leyes como una forma de transformar las categorías científicas en las soluciones de creación de leyes

La institucionalización de los principios de creación de la ley es la tarea más importante del estado que se otorga a la formación orientada hacia la creación de la ley, para hacer cumplir el propósito social y las formas de desarrollo del estado legalment

A choice of legal act during law-making process

There is a problem of legal act choice during the law-making process considered in the paper. A method for step-by-step choice algorithm is advanced. The algorithm is associated with public relationship assessment. The public relationships to be regulated must have a certain social valu

Estimated Inpatient Hospital Stay in Individual Wards: Guidelines on Radiation Safety after Radioiodine Therapy

Radionuclide therapy safety requirements are regulated by the Russian Radiation Safety Standards (RRSS), which state the maximum allowed radionuclide activity in the body and the equivalent dose rate (EDR) of gamma radiation. Therefore, it is necessary to estimate the time of an inpatient hospital stay in specially designed radionuclide therapy wards. The article presents the findings of individual 131I biokinetics studies in 64 patients admitted to radioiodine therapy of thyrotoxicosis and differentiated thyroid cancer. We developed a method to calculate the time interval to reach the EDR of 20μSv/h and the recommended EDR of 3 and 0.3μSv/h for adults and children, respectively. It is based on the measurement of the 131I excretion constant. Keywords: Radioiodine therapy, Radiation safety, Guidelin