Rearrangement of the Keratin Cytoskeleton after Combined Treatment with Microtubule and Microfilament Inhibitors

In addition to containing microtubule and microfilament systems, vertebrate epithelial cells contain an elaborate keratin intermediate-filament cytoskeleton. Little is known about its structural organization or function. Using indirect immunofluorescence microscopy with an antikeratin antiserum probe, we found that destabilization of microtubules and microfilaments with cytostatic drugs induces significant alterations in the cytoskeletal organizationof keratin filaments in HeLa and fetal mouse epidermal cells. Keratin filament organizationwas observed to undergo a rapid (1-2 h) transition from a uniform distribution to an open lattice of keratin fibers stabilized by membrane-associated focal centers . Since addition of any one drug alone did not elicit significant organizational change in the keratin cytoskeleton,we suggest that microfilaments and microtubules have a combined role in maintaining the arrangement of keratin in these cells. Vimentin filaments, the only other intermediate sized filaments found in HeLa cells, did not co-distribute with keratin in untreated or drug treated cells. These findings offer a new way to approach the study of the dynamics and functional roles of the keratin cytoskeleton in epithelial cells

Adversarially Learned Anomaly Detection on CMS open data: re-discovering the top quark

We apply an Adversarially Learned Anomaly Detection (ALAD) algorithm to the problem of detecting new physics processes in proton–proton collisions at the Large Hadron Collider. Anomaly detection based on ALAD matches performances reached by Variational Autoencoders, with a substantial improvement in some cases. Training the ALAD algorithm on 4.4 fb⁻¹ of 8 TeV CMS Open Data, we show how a data-driven anomaly detection and characterization would work in real life, re-discovering the top quark by identifying the main features of the tt̄ experimental signature at the LHC

An optimized intermolecular force field for hydrogen bonded organic molecular crystals using atomic multipole electrostatics

This is the author accepted manuscript. It is currently under an indefinite embargo pending publication by the International Union of Crystallography.We present a re-parameterization of the a popular intermolecular force field for describing intermolecular interactions in the organic solid state. Specifically, we optimize the performance of the exp-6 force field when used in conjunction with atomic multipole electrostatics. We also parameterize force fields that are optimized for use with multipoles derived from polarized molecular electron densities, to account for induction effects in molecular crystals. Parameterization is performed against a set of 186 experimentally determined, low temperature crystal structures and 53 measured sublimation enthalpies of hydrogen bonding organic molecules. The resulting force fields are tested on a validation set of 129 crystal structures and show improved reproduction of the structures and lattice energies of a range of organic molecular crystals compared to the original force field with atomic partial charge electrostatics. Unit cell dimensions of the validation set are typically reproduced to within 3% with the re-parameterized force fields. Lattice energies, which were all included during parameterisation, are systematically underestimated when compared to measured sublimation enthalpies, with mean absolute errors of between 7.4 and 9.0%.Engineering and Physical Sciences Research Council (Doctoral Training Account studentship

A Macroscopic Description of Coherent Geo-Magnetic Radiation from Cosmic Ray Air Showers

We have developed a macroscopic description of coherent electro-magnetic radiation from air showers initiated by ultra-high energy cosmic rays due to the presence of the geo-magnetic field. This description offers a simple and direct insight in the relation between the properties of the air shower and the time-structure of the radio pulse.Comment: 10 pages, 8 figures; submitted to Astroparticle Physic