Association of recurrent pregnancy loss with chromosomal abnormalities and hereditary thrombophilias

Background: Recurrent pregnancy loss (RPL) which is generally known as >3 consecutive pregnancy losses before 20 weeks’ gestation is seen in 0.5-2% of women Objective: To evaluate the association of parental and fetal chromosomal abnormalities with recurrent pregnancy loss in our area and to analyze the frequency of three types of hereditary thrombophilia’s; (MTHFR C677T polymorphisms, FV Leiden G1691A mutation and Prothrombin (factor II) G20210A mutation) in these female patients. Methods: The present case-control retrospective study was performed between February 2007 and December 2011 on 495 couples, who had two or more consecutive pregnancy losses before 20 weeks’ gestation. We used conventional cytogenetic analysis and polymerase chain reaction–restriction fragment length polymorphism. Results: Parental chromosomal abnormality was detected in 28 cases (2.8% of all cases, 5.7% of the couples) most of which (92.9%) were structural abnormalities. All of the structural abnormalities were balanced chromosomal translocations. Chromosomal analysis performed from the abortion materials detected a major chromosomal abnormality in 31.9% of the cases. The most frequently observed alteration in the hereditary thrombophilia genes was heterozygote mutation for the MTHFR C677T polymorphisms (n=55). Conclusion: Balanced translocations are the most commonly detected chromosomal abnormalities in couples being evaluated for recurrent pregnancy loss and these patients are the best candidates for offering prenatal genetic diagnosis by the help of which there is a possibility of obtaining a better reproductive outcome

Effect of Hatching Time on Yolk Sac Percentage and Broiler Live Performance

ABSTRACT This study investigated the effects of broiler chick hatching time on the percentage of the yolk sac and subsequent broiler live performance. Broiler hatching eggs were obtained from a commercial flock at 55 wk of age and were stored for 2 d at 18°C and 75% relative humidity (RH) prior to incubation. Chicks were identified as hatching Early (471-477 h), Middle (480-486 h), and Late (494-510 h). All chicks were removed from the trays at 510 h of incubation. Body weight (BW) and yolk weight (YW) were determined at emergence from the shell (initial hatch time) and at placement on feed. Chicks were permanently identified by hatch time with neck tags, feather sexed, weighed, and introduced to feed and water in litter floor pens. Broiler BW and feed consumption (FC) were then determined 7, 21, and 35 d of age. Mortality was recorded daily. Although the percentage of the yolk was similar at hatch time, it was less in Early compared to Middle and Late chicks at placement (p≤0.05). Broiler chick BW was greater at placement in Late chicks compared to Early and Middle chicks (p≤0.05) but this advantage disappeared by 7 d because Late chicks consumed less feed to 7 d (p≤0.05). Early hatched broilers exhibited greater BW than Late with Middle broilers intermediate at 35 d (p≤0.05). There were no significant differences in feed consumption and feed conversion ratio (FCR) at 35 d of age. Additionally, late hatch chicks exhibited greater mortality. Overall, live performance of Late hatching chicks, judged by mortality and BW at 35 d, was reduced compared to Early hatching chicks

Bilateral superficial cervical plexus block combined with bilateral greater occipital nerve block reduces the impact of different sources of pain after thyroid surgery with less consumption of morphine-PCA: a randomized trial

Bayir, Omer/0000-0001-9445-6129WOS: 000358736300006Background Our aim was to demonstrate the analgesic efficacy of the bilateral superficial cervical plexus block (BSCPB) combined with bilateral greater occipital nerve block (BGONB) on different sources of pain after thyroid surgery; in terms of pain scores and morphine-PCA consumption in the first 24 h. Materials and method Sixty patients were randomized into three groups; Group C (n = 20) to receive management without blocks; Group GS (n = 20), the combination of BSCPB and BGONB; and Group S (n = 20), BSCPB alone. Levobupivacaine of 0.25 % (15 ml (each side) for BSCPB; 5 ml (each side) BGONB) was used for nerve blocks. All patients received morphine-PCA for the first 24 h postoperatively. In all, 0 h, 1 h, 2 h, 4 h, 6 h, 12 h, and 24 h were the measurement times for incision pain at rest, pain on swallowing, headache, and posterior neck pain scores (VAS) and PONV. Requirement for rescue analgesics and 24 h morphine consumption were recorded. Results Incision pain scores at rest and on swallowing was lower in Groups GS and S (p 3 was lower in Group GS (p < 0.001). In Groups GS and S 24 h morphine consumption was lower, compared to Group C (p < 0.001). PONV and rescue analgesic use were similar. Conclusion The combination of three-injection technique BSCPB with BGONB performed before surgical incision by using 0.25 % levobupivacaine, significantly reduced incision pain at rest, incision pain on swallowing, headache and posterior neck pain after thyroid surgery, while reducing morphine consumption in 24 h postoperatively

Evaluation of 21 infants with arrythmia in the neonatal period [Yenidogan döneminde aritmi gelişen 21 vakanin degerlendirilmesi]

Perinatal arrythmia is described as abnormal heart rates or existence of extrasystoles in the fetal or neonatal period. In this study, neonates admitted to the Çukurova University Neonatal Intensive Care Unit between 1 January 1998-30 June 2003 for arrythmia or who were diagnosed as arrythmia after hospitalization were evaluated retrospectively. In this period, 21 of 4465 patients (0.4% of all patients) was diagnosed as arrythmia. Supraventricular tachycardia were determined in 12 (57.1%), ventricular tachycardia in two (9.5%), and ventricular fibrillation in two (9.5%), atrioventricular block in five (23.8%) patients. When infants in the neonatal intensive care units are classfied according to diagnosis, frequency of arrythmia is low. Although arrythmia in the neonatal period is rare, treatment must be urgent

Broken umbilical artery catheter: A case report [Umbilikal kateterizasyonu iliskili seyrek görülen bir komplikasyon]

Umbilical venous catheterization is frequently used for treatment, monitorization, blood sampling and total parenteral nutrition in neonatal intensive care units. During umbilical catheterization, complications such as occlusion, bleeding, breaking, leaking, thrombosis, infection, distal embolization, perforation and cardiac tamponade may be observed. For this reason great attendence should be given to catheter complications especially during catheterization and catheter removal. Accidentally cutting or breaking of catheter may be observed. The catheter piece inside can be removed by transumbilical catheterization or surgical attempt. Here we report a preterm infant with 28 gestational weeks of age whose umbilical catheter was broken or accidentally cut during removal of catheter. The piece of catheter inside was removed surgically

Renal Biopsy Prognostic Findings in Children With Atypical Hemolytic Uremic Syndrome.

BACKGROUND: The aim of this study was to investigate the histopathological findings in kidney biopsies in children with atypical hemolytic uremic syndrome (aHUS) and to determine whether specific pathological findings in aHUS have a prognostic value. METHODS: Renal biopsy specimens of 29 patients who were recorded in the national Turkish aHUS registry database were available for review. Histopathological findings were compared with the clinical and laboratory features at the presentation and the final outcome. RESULTS: The mean age at presentation and follow-up period was 4.9 ± 3.9 and 3.9 ± 3.0 years, respectively. The median time interval from the first symptom to biopsy was 10 days. Vascular thrombosis and interstitial fibrosis were significantly related to chronic kidney disease (CKD) requiring dialysis or kidney transplantation during follow-up (5.6-fold, for both). Glomerular necrosis, cortical necrosis, and glomerular sclerosis were markedly associated with CKD without dialysis (6.2-fold, 13.3-fold, and 8.8-fold, respectively). However, presence of endothelial swelling, subendothelial widening, and fragmented erythrocytes was found to be correlated with a favorable final outcome. CONCLUSIONS: Presence of vascular thrombosis, cortical necrosis, and glomerular sclerosis in histopathological evaluation correlated with developing CKD. Chronic changes in the interstitial compartment were also related to poor prognosis, a finding that has been shown for the first time in pediatric aHUS cases